Amino acid ester synthesis by immobilized α-chymotrypsin
(1987) In Biotechnology Letters 9. p.455-460- Abstract
- A screening of immobilized α-chymotrypsin preparations suitable for the synthesis of N-acetyl-L-tyrosine ethyl ester from N-acetyl-L-tyrosine and up to 99% ethanol was carried out. α-Chymotrypsin adsorbed to Sepharose LH-20 or covalently bound to Sepharose 4B (tresyl chloride activation) was found to be an efficient catalyst. A column packed with immobilized enzyme retained 60% of its initial activity after 6 days of operation in a cyclohexane-ethanol medium.
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https://lup.lub.lu.se/record/ec59f7f2-1433-4d7d-9db8-44a283915e4b
- author
- Mori, Takao ; Nilsson, Kurt ; Larsson, Per-Olof LU and Mosbach, Klaus LU
- organization
- publishing date
- 1987
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Immobilized chymotrypsin, Esterification in 99 % ethanol, Stable enzyme
- in
- Biotechnology Letters
- volume
- 9
- pages
- 6 pages
- publisher
- Springer
- external identifiers
-
- scopus:0343405289
- ISSN
- 0951-208X
- DOI
- 10.1007/BF01027451
- language
- English
- LU publication?
- yes
- id
- ec59f7f2-1433-4d7d-9db8-44a283915e4b
- date added to LUP
- 2024-06-18 14:17:14
- date last changed
- 2024-09-04 09:52:02
@article{ec59f7f2-1433-4d7d-9db8-44a283915e4b, abstract = {{A screening of immobilized α-chymotrypsin preparations suitable for the synthesis of N-acetyl-L-tyrosine ethyl ester from N-acetyl-L-tyrosine and up to 99% ethanol was carried out. α-Chymotrypsin adsorbed to Sepharose LH-20 or covalently bound to Sepharose 4B (tresyl chloride activation) was found to be an efficient catalyst. A column packed with immobilized enzyme retained 60% of its initial activity after 6 days of operation in a cyclohexane-ethanol medium.}}, author = {{Mori, Takao and Nilsson, Kurt and Larsson, Per-Olof and Mosbach, Klaus}}, issn = {{0951-208X}}, keywords = {{Immobilized chymotrypsin; Esterification in 99 % ethanol; Stable enzyme}}, language = {{eng}}, pages = {{455--460}}, publisher = {{Springer}}, series = {{Biotechnology Letters}}, title = {{Amino acid ester synthesis by immobilized α-chymotrypsin}}, url = {{http://dx.doi.org/10.1007/BF01027451}}, doi = {{10.1007/BF01027451}}, volume = {{9}}, year = {{1987}}, }