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Amino acid ester synthesis by immobilized α-chymotrypsin

Mori, Takao ; Nilsson, Kurt ; Larsson, Per-Olof LU and Mosbach, Klaus LU (1987) In Biotechnology Letters 9. p.455-460
Abstract
A screening of immobilized α-chymotrypsin preparations suitable for the synthesis of N-acetyl-L-tyrosine ethyl ester from N-acetyl-L-tyrosine and up to 99% ethanol was carried out. α-Chymotrypsin adsorbed to Sepharose LH-20 or covalently bound to Sepharose 4B (tresyl chloride activation) was found to be an efficient catalyst. A column packed with immobilized enzyme retained 60% of its initial activity after 6 days of operation in a cyclohexane-ethanol medium.
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Immobilized chymotrypsin, Esterification in 99 % ethanol, Stable enzyme
in
Biotechnology Letters
volume
9
pages
6 pages
publisher
Springer Science and Business Media B.V.
external identifiers
  • scopus:0343405289
ISSN
0951-208X
DOI
10.1007/BF01027451
language
English
LU publication?
yes
id
ec59f7f2-1433-4d7d-9db8-44a283915e4b
date added to LUP
2024-06-18 14:17:14
date last changed
2025-10-14 10:30:09
@article{ec59f7f2-1433-4d7d-9db8-44a283915e4b,
  abstract     = {{A screening of immobilized α-chymotrypsin preparations suitable for the synthesis of N-acetyl-L-tyrosine ethyl ester from N-acetyl-L-tyrosine and up to 99% ethanol was carried out. α-Chymotrypsin adsorbed to Sepharose LH-20 or covalently bound to Sepharose 4B (tresyl chloride activation) was found to be an efficient catalyst. A column packed with immobilized enzyme retained 60% of its initial activity after 6 days of operation in a cyclohexane-ethanol medium.}},
  author       = {{Mori, Takao and Nilsson, Kurt and Larsson, Per-Olof and Mosbach, Klaus}},
  issn         = {{0951-208X}},
  keywords     = {{Immobilized chymotrypsin; Esterification in 99 % ethanol; Stable enzyme}},
  language     = {{eng}},
  pages        = {{455--460}},
  publisher    = {{Springer Science and Business Media B.V.}},
  series       = {{Biotechnology Letters}},
  title        = {{Amino acid ester synthesis by immobilized α-chymotrypsin}},
  url          = {{http://dx.doi.org/10.1007/BF01027451}},
  doi          = {{10.1007/BF01027451}},
  volume       = {{9}},
  year         = {{1987}},
}