Schisandrin B Suppresses Colon Cancer Growth by Inducing Cell Cycle Arrest and Apoptosis : Molecular Mechanism and Therapeutic Potential
(2024) In ACS Pharmacology and Translational Science 7(3). p.863-877- Abstract
Colon cancer is among the most lethal and prevalent malignant tumors in the world, and the lack of effective therapies highlights the need for novel therapeutic approaches. Schisandrin B (Sch B), a lignan extracted from the fruit ofSchisandra chinensis, has been reported for its anticancer properties. However, to date, no studies have been done to characterize the exact molecular mechanisms underlying the antitumorigenic effects of Sch B in colon cancer. This study aimed to explore the antitumorigenic effects of Sch B in colon cancer and to understand the underlying therapeutic mechanism. A comprehensive analysis of the molecular mechanism underlying the antitumorigenic effects of Sch B on human colon cancer cells was performed using a... (More)
Colon cancer is among the most lethal and prevalent malignant tumors in the world, and the lack of effective therapies highlights the need for novel therapeutic approaches. Schisandrin B (Sch B), a lignan extracted from the fruit ofSchisandra chinensis, has been reported for its anticancer properties. However, to date, no studies have been done to characterize the exact molecular mechanisms underlying the antitumorigenic effects of Sch B in colon cancer. This study aimed to explore the antitumorigenic effects of Sch B in colon cancer and to understand the underlying therapeutic mechanism. A comprehensive analysis of the molecular mechanism underlying the antitumorigenic effects of Sch B on human colon cancer cells was performed using a combination of Raman spectroscopy, RNA-seq, computational docking, and molecular biological experiments. The in vivo efficacy was evaluated by a mouse xenograft model. Sch B reduced cell proliferation and triggered apoptosis in human colon cancer cell lines. Raman spectroscopy, computational, RNA-seq, and molecular and cellular studies revealed that Sch B activated unfolded protein responses by interacting with CHOP and upregulating CHOP, which thereby induced apoptosis. CHOP knockdown alleviated the Sch B-induced reduction in cell viability and apoptosis. Sch B reduced colon tumor growth in vivo. Our findings demonstrated that Sch B induced apoptosis and inhibited cell proliferation and tumor growth in vitro and in vivo. These results provided an essential background for clinical trials examining the effects of Sch B in patients with colon cancer.
(Less)
- author
- organization
- publishing date
- 2024-03
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- apoptosis, cell cycle arrest, molecular docking, novel colon cancer treatment, Raman spectral change, schisandrin B
- in
- ACS Pharmacology and Translational Science
- volume
- 7
- issue
- 3
- pages
- 15 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:38481680
- scopus:85186203217
- DOI
- 10.1021/acsptsci.4c00009
- language
- English
- LU publication?
- yes
- id
- ec74689e-6433-4059-877a-69d3d07df36d
- date added to LUP
- 2024-03-25 12:56:10
- date last changed
- 2024-04-22 15:11:05
@article{ec74689e-6433-4059-877a-69d3d07df36d,
abstract = {{<p>Colon cancer is among the most lethal and prevalent malignant tumors in the world, and the lack of effective therapies highlights the need for novel therapeutic approaches. Schisandrin B (Sch B), a lignan extracted from the fruit ofSchisandra chinensis, has been reported for its anticancer properties. However, to date, no studies have been done to characterize the exact molecular mechanisms underlying the antitumorigenic effects of Sch B in colon cancer. This study aimed to explore the antitumorigenic effects of Sch B in colon cancer and to understand the underlying therapeutic mechanism. A comprehensive analysis of the molecular mechanism underlying the antitumorigenic effects of Sch B on human colon cancer cells was performed using a combination of Raman spectroscopy, RNA-seq, computational docking, and molecular biological experiments. The in vivo efficacy was evaluated by a mouse xenograft model. Sch B reduced cell proliferation and triggered apoptosis in human colon cancer cell lines. Raman spectroscopy, computational, RNA-seq, and molecular and cellular studies revealed that Sch B activated unfolded protein responses by interacting with CHOP and upregulating CHOP, which thereby induced apoptosis. CHOP knockdown alleviated the Sch B-induced reduction in cell viability and apoptosis. Sch B reduced colon tumor growth in vivo. Our findings demonstrated that Sch B induced apoptosis and inhibited cell proliferation and tumor growth in vitro and in vivo. These results provided an essential background for clinical trials examining the effects of Sch B in patients with colon cancer.</p>}},
author = {{Co, Vanessa Anna and El-Nezami, Hani and Liu, Yawen and Twum, Bonsra and Dey, Priyanka and Cox, Paul A. and Joseph, Shalu and Agbodjan-Dossou, Roland and Sabzichi, Mehdi and Draheim, Roger and Wan, Murphy Lam Yim}},
keywords = {{apoptosis; cell cycle arrest; molecular docking; novel colon cancer treatment; Raman spectral change; schisandrin B}},
language = {{eng}},
number = {{3}},
pages = {{863--877}},
publisher = {{The American Chemical Society (ACS)}},
series = {{ACS Pharmacology and Translational Science}},
title = {{Schisandrin B Suppresses Colon Cancer Growth by Inducing Cell Cycle Arrest and Apoptosis : Molecular Mechanism and Therapeutic Potential}},
url = {{http://dx.doi.org/10.1021/acsptsci.4c00009}},
doi = {{10.1021/acsptsci.4c00009}},
volume = {{7}},
year = {{2024}},
}