In Vitro Studies of Persister Cells
(2020) In Microbiology and Molecular Biology Reviews 84(4).- Abstract
SUMMARY Many bacterial pathogens can permanently colonize their host and establish either chronic or recurrent infections that the immune system and antimicrobial therapies fail to eradicate. Antibiotic persisters (persister cells) are believed to be among the factors that make these infections challenging. Persisters are subpopulations of bacteria which survive treatment with bactericidal antibiotics in otherwise antibiotic-sensitive cultures and were extensively studied in a hope to discover the mechanisms that cause treatment failures in chronically infected patients; however, most of these studies were conducted in the test tube. Research into antibiotic persistence has uncovered large intrapopulation heterogeneity of bacterial... (More)
SUMMARY Many bacterial pathogens can permanently colonize their host and establish either chronic or recurrent infections that the immune system and antimicrobial therapies fail to eradicate. Antibiotic persisters (persister cells) are believed to be among the factors that make these infections challenging. Persisters are subpopulations of bacteria which survive treatment with bactericidal antibiotics in otherwise antibiotic-sensitive cultures and were extensively studied in a hope to discover the mechanisms that cause treatment failures in chronically infected patients; however, most of these studies were conducted in the test tube. Research into antibiotic persistence has uncovered large intrapopulation heterogeneity of bacterial growth and regrowth but has not identified essential, dedicated molecular mechanisms of antibiotic persistence. Diverse factors and stresses that inhibit bacterial growth reduce killing of the bulk population and may also increase the persister subpopulation, implying that an array of mechanisms are present. Hopefully, further studies under conditions that simulate the key aspects of persistent infections will lead to identifying target mechanisms for effective therapeutic solutions.
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- author
- Kaldalu, Niilo ; Hauryliuk, Vasili LU ; Turnbull, Kathryn Jane ; Mensa, Agnese La ; Putrinš, Marta and Tensona, Tanel
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- keywords
- Antibiotic resistance, Antimicrobial agents, Persistence
- in
- Microbiology and Molecular Biology Reviews
- volume
- 84
- issue
- 4
- article number
- 00070-20
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:33177189
- scopus:85096082977
- ISSN
- 1092-2172
- DOI
- 10.1128/MMBR.00070-20
- language
- English
- LU publication?
- no
- additional info
- Funding Information: The work was supported by the European Union from the European Regional Development Fund through the Centre of Excellence in Molecular Cell Engineering (2014-2020.4.01.15-0013), by the European Commission (EC) via project MIBEst H2020-WIDESPREAD-2018-2020/GA number 857518, and by a grant from the Estonian Research Council (PRG335) to T.T. N.K was supported by the Institute of Technology basic funding grant. V.H. was supported by the Swedish Research council via grants 2017-03783 (project grant) and 2018-00956 (within the framework of JPIAMR consortium grant RIBOTARGET). Publisher Copyright: Copyright © 2020 American Society for Microbiology. All Rights Reserved. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
- id
- ecbd6990-6611-4a18-aaff-1d39a4bedf17
- date added to LUP
- 2021-09-24 20:31:39
- date last changed
- 2024-04-20 12:53:58
@article{ecbd6990-6611-4a18-aaff-1d39a4bedf17, abstract = {{<p>SUMMARY Many bacterial pathogens can permanently colonize their host and establish either chronic or recurrent infections that the immune system and antimicrobial therapies fail to eradicate. Antibiotic persisters (persister cells) are believed to be among the factors that make these infections challenging. Persisters are subpopulations of bacteria which survive treatment with bactericidal antibiotics in otherwise antibiotic-sensitive cultures and were extensively studied in a hope to discover the mechanisms that cause treatment failures in chronically infected patients; however, most of these studies were conducted in the test tube. Research into antibiotic persistence has uncovered large intrapopulation heterogeneity of bacterial growth and regrowth but has not identified essential, dedicated molecular mechanisms of antibiotic persistence. Diverse factors and stresses that inhibit bacterial growth reduce killing of the bulk population and may also increase the persister subpopulation, implying that an array of mechanisms are present. Hopefully, further studies under conditions that simulate the key aspects of persistent infections will lead to identifying target mechanisms for effective therapeutic solutions.</p>}}, author = {{Kaldalu, Niilo and Hauryliuk, Vasili and Turnbull, Kathryn Jane and Mensa, Agnese La and Putrinš, Marta and Tensona, Tanel}}, issn = {{1092-2172}}, keywords = {{Antibiotic resistance; Antimicrobial agents; Persistence}}, language = {{eng}}, number = {{4}}, publisher = {{American Society for Microbiology}}, series = {{Microbiology and Molecular Biology Reviews}}, title = {{In Vitro Studies of Persister Cells}}, url = {{http://dx.doi.org/10.1128/MMBR.00070-20}}, doi = {{10.1128/MMBR.00070-20}}, volume = {{84}}, year = {{2020}}, }