Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II
(2014) In Journal of Cell Biology 206(7). p.843-853- Abstract
In mitosis, the Greatwall kinase (called microtubuleassociated serine/threonine kinase like [Mastl] in mammals)is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely... (More)
In mitosis, the Greatwall kinase (called microtubuleassociated serine/threonine kinase like [Mastl] in mammals)is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.
(Less)
- author
- publishing date
- 2014-09-29
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Cell Biology
- volume
- 206
- issue
- 7
- pages
- 843 - 853
- publisher
- Rockefeller University Press
- external identifiers
-
- pmid:25246615
- scopus:84907764457
- ISSN
- 0021-9525
- DOI
- 10.1083/jcb.201406033
- language
- English
- LU publication?
- no
- id
- ecd585bc-0508-4626-87ed-26012a6ad213
- date added to LUP
- 2019-09-18 13:53:03
- date last changed
- 2024-10-16 15:26:21
@article{ecd585bc-0508-4626-87ed-26012a6ad213, abstract = {{<p>In mitosis, the Greatwall kinase (called microtubuleassociated serine/threonine kinase like [Mastl] in mammals)is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.</p>}}, author = {{Adhikari, Deepak and Diril, M. Kasim and Busayavalasa, Kiran and Risal, Sanjiv and Nakagawa, Shoma and Lindkvist, Rebecca and Shen, Yan and Coppola, Vincenzo and Tessarollo, Lino and Kudo, Nobuaki R. and Kaldis, Philipp and Liu, Kui}}, issn = {{0021-9525}}, language = {{eng}}, month = {{09}}, number = {{7}}, pages = {{843--853}}, publisher = {{Rockefeller University Press}}, series = {{Journal of Cell Biology}}, title = {{Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II}}, url = {{http://dx.doi.org/10.1083/jcb.201406033}}, doi = {{10.1083/jcb.201406033}}, volume = {{206}}, year = {{2014}}, }