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Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II

Adhikari, Deepak ; Diril, M. Kasim ; Busayavalasa, Kiran ; Risal, Sanjiv ; Nakagawa, Shoma ; Lindkvist, Rebecca ; Shen, Yan ; Coppola, Vincenzo ; Tessarollo, Lino and Kudo, Nobuaki R. , et al. (2014) In Journal of Cell Biology 206(7). p.843-853
Abstract

In mitosis, the Greatwall kinase (called microtubuleassociated serine/threonine kinase like [Mastl] in mammals)is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely... (More)

In mitosis, the Greatwall kinase (called microtubuleassociated serine/threonine kinase like [Mastl] in mammals)is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.

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publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Cell Biology
volume
206
issue
7
pages
843 - 853
publisher
Rockefeller University Press
external identifiers
  • pmid:25246615
  • scopus:84907764457
ISSN
0021-9525
DOI
10.1083/jcb.201406033
language
English
LU publication?
no
id
ecd585bc-0508-4626-87ed-26012a6ad213
date added to LUP
2019-09-18 13:53:03
date last changed
2024-01-01 20:34:28
@article{ecd585bc-0508-4626-87ed-26012a6ad213,
  abstract     = {{<p>In mitosis, the Greatwall kinase (called microtubuleassociated serine/threonine kinase like [Mastl] in mammals)is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.</p>}},
  author       = {{Adhikari, Deepak and Diril, M. Kasim and Busayavalasa, Kiran and Risal, Sanjiv and Nakagawa, Shoma and Lindkvist, Rebecca and Shen, Yan and Coppola, Vincenzo and Tessarollo, Lino and Kudo, Nobuaki R. and Kaldis, Philipp and Liu, Kui}},
  issn         = {{0021-9525}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{7}},
  pages        = {{843--853}},
  publisher    = {{Rockefeller University Press}},
  series       = {{Journal of Cell Biology}},
  title        = {{Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II}},
  url          = {{http://dx.doi.org/10.1083/jcb.201406033}},
  doi          = {{10.1083/jcb.201406033}},
  volume       = {{206}},
  year         = {{2014}},
}