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Storage lipid synthesis is non-essential in yeast

Sandager, L ; Gustavsson, Maria LU ; Stahl, U ; Dahlqvist, A ; Wiberg, E ; Banas, A ; Lenman, Marit LU ; Ronne, H and Stymne, S (2002) In Journal of Biological Chemistry 277(8). p.6478-6482
Abstract
Steryl esters and triacylglycerol (TAG) are the main storage lipids in eukaryotic cells. In the yeast Saccharomyces cerevisiae, these storage lipids accumulate during stationary growth phase within organelles known as lipid bodies. We have used single and multiple gene disruptions to study storage lipid synthesis in yeast. Four genes, ARE1, ARE2, DGA1, and LRO1, were found to contribute to TAG synthesis. The most significant contribution is made by DGA1, which encodes a novel acyl-CoA;diacylglycerol acyltransferase. Two of the genes, ARE1 and ARE2, are also involved in steryl ester synthesis. A yeast strain that lacks all four genes is viable and has no apparent growth defects under standard conditions. The strain is devoid of both TAG and... (More)
Steryl esters and triacylglycerol (TAG) are the main storage lipids in eukaryotic cells. In the yeast Saccharomyces cerevisiae, these storage lipids accumulate during stationary growth phase within organelles known as lipid bodies. We have used single and multiple gene disruptions to study storage lipid synthesis in yeast. Four genes, ARE1, ARE2, DGA1, and LRO1, were found to contribute to TAG synthesis. The most significant contribution is made by DGA1, which encodes a novel acyl-CoA;diacylglycerol acyltransferase. Two of the genes, ARE1 and ARE2, are also involved in steryl ester synthesis. A yeast strain that lacks all four genes is viable and has no apparent growth defects under standard conditions. The strain is devoid of both TAG and steryl esters, and fluorescence microscopy revealed that it also lacks lipid bodies. We conclude that neither storage lipids nor lipid bodies are essential for growth in yeast. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
277
issue
8
pages
6478 - 6482
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • wos:000173989200103
  • pmid:11741946
  • scopus:0037155261
ISSN
1083-351X
DOI
10.1074/jbc.M109109200
language
English
LU publication?
yes
id
ed404362-3e52-4f95-b66c-d3881e25b1b8 (old id 342865)
date added to LUP
2016-04-01 11:46:54
date last changed
2022-04-05 05:01:00
@article{ed404362-3e52-4f95-b66c-d3881e25b1b8,
  abstract     = {{Steryl esters and triacylglycerol (TAG) are the main storage lipids in eukaryotic cells. In the yeast Saccharomyces cerevisiae, these storage lipids accumulate during stationary growth phase within organelles known as lipid bodies. We have used single and multiple gene disruptions to study storage lipid synthesis in yeast. Four genes, ARE1, ARE2, DGA1, and LRO1, were found to contribute to TAG synthesis. The most significant contribution is made by DGA1, which encodes a novel acyl-CoA;diacylglycerol acyltransferase. Two of the genes, ARE1 and ARE2, are also involved in steryl ester synthesis. A yeast strain that lacks all four genes is viable and has no apparent growth defects under standard conditions. The strain is devoid of both TAG and steryl esters, and fluorescence microscopy revealed that it also lacks lipid bodies. We conclude that neither storage lipids nor lipid bodies are essential for growth in yeast.}},
  author       = {{Sandager, L and Gustavsson, Maria and Stahl, U and Dahlqvist, A and Wiberg, E and Banas, A and Lenman, Marit and Ronne, H and Stymne, S}},
  issn         = {{1083-351X}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{6478--6482}},
  publisher    = {{American Society for Biochemistry and Molecular Biology}},
  series       = {{Journal of Biological Chemistry}},
  title        = {{Storage lipid synthesis is non-essential in yeast}},
  url          = {{http://dx.doi.org/10.1074/jbc.M109109200}},
  doi          = {{10.1074/jbc.M109109200}},
  volume       = {{277}},
  year         = {{2002}},
}