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Inducible nitric oxide synthase expression is increased in the alveolar compartment of asthmatic patients

Tufvesson, E. LU ; Andersson, C.; Weidner, J. LU ; Erjefält, J. S. LU and Bjermer, L. LU (2017) In Allergy: European Journal of Allergy and Clinical Immunology 72(4). p.627-635
Abstract

Introduction: Increased exhaled nitric oxide (NO) levels in asthma are suggested to be through inducible NO synthase (iNOS). The aim of this study was to investigate the expression of iNOS in bronchoalveolar lavage (BAL) cells and tissue from central and peripheral airways and compare it with the exhaled bronchial and alveolar NO levels in patients with asthma vs a control group. Methods: Thirty-two patients with asthma (defined as controlled or uncontrolled according to Asthma Control Test score cut-off: 20) and eight healthy controls were included. Exhaled NO was measured, and alveolar concentration and bronchial flux were calculated. iNOS was measured in central and peripheral lung biopsies, as well as BAL cells. Bronchoalveolar... (More)

Introduction: Increased exhaled nitric oxide (NO) levels in asthma are suggested to be through inducible NO synthase (iNOS). The aim of this study was to investigate the expression of iNOS in bronchoalveolar lavage (BAL) cells and tissue from central and peripheral airways and compare it with the exhaled bronchial and alveolar NO levels in patients with asthma vs a control group. Methods: Thirty-two patients with asthma (defined as controlled or uncontrolled according to Asthma Control Test score cut-off: 20) and eight healthy controls were included. Exhaled NO was measured, and alveolar concentration and bronchial flux were calculated. iNOS was measured in central and peripheral lung biopsies, as well as BAL cells. Bronchoalveolar lavage macrophages were stimulated in vitro, and iNOS expression and NO production were investigated. Results: Expression of iNOS was increased in central airway tissue and the alveolar compartment in uncontrolled as compared to controlled asthmatics and healthy controls. There were no differences, however, in iNOS mRNA levels in total BAL cells in uncontrolled as compared to controlled asthma. Bronchoalveolar lavage cell mRNA levels of iNOS or iNOS expression in central and alveolar tissue did not relate to alveolar NO, nor to bronchial flux of NO. In vitro stimulation with leukotriene D4 increased iNOS mRNA levels and NO production in cultured BAL macrophages. Conclusion: The levels of both bronchial and alveolar iNOS are increased in uncontrolled as compared to controlled asthma. However, levels of iNOS in BAL macrophages were not reflected by alveolar NO. Both central and distal iNOS levels may reflect responsiveness to steroid treatment.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Asthma, Exhaled nitric oxide, Inducible nitric oxide synthase
in
Allergy: European Journal of Allergy and Clinical Immunology
volume
72
issue
4
pages
627 - 635
publisher
Wiley-Blackwell
external identifiers
  • scopus:84992398682
  • wos:000397489400013
ISSN
0105-4538
DOI
10.1111/all.13052
language
English
LU publication?
yes
id
ed44f435-e102-498c-843d-04fdfcae768f
date added to LUP
2016-11-16 16:23:10
date last changed
2018-01-07 11:35:36
@article{ed44f435-e102-498c-843d-04fdfcae768f,
  abstract     = {<p>Introduction: Increased exhaled nitric oxide (NO) levels in asthma are suggested to be through inducible NO synthase (iNOS). The aim of this study was to investigate the expression of iNOS in bronchoalveolar lavage (BAL) cells and tissue from central and peripheral airways and compare it with the exhaled bronchial and alveolar NO levels in patients with asthma vs a control group. Methods: Thirty-two patients with asthma (defined as controlled or uncontrolled according to Asthma Control Test score cut-off: 20) and eight healthy controls were included. Exhaled NO was measured, and alveolar concentration and bronchial flux were calculated. iNOS was measured in central and peripheral lung biopsies, as well as BAL cells. Bronchoalveolar lavage macrophages were stimulated in vitro, and iNOS expression and NO production were investigated. Results: Expression of iNOS was increased in central airway tissue and the alveolar compartment in uncontrolled as compared to controlled asthmatics and healthy controls. There were no differences, however, in iNOS mRNA levels in total BAL cells in uncontrolled as compared to controlled asthma. Bronchoalveolar lavage cell mRNA levels of iNOS or iNOS expression in central and alveolar tissue did not relate to alveolar NO, nor to bronchial flux of NO. In vitro stimulation with leukotriene D<sub>4</sub> increased iNOS mRNA levels and NO production in cultured BAL macrophages. Conclusion: The levels of both bronchial and alveolar iNOS are increased in uncontrolled as compared to controlled asthma. However, levels of iNOS in BAL macrophages were not reflected by alveolar NO. Both central and distal iNOS levels may reflect responsiveness to steroid treatment.</p>},
  author       = {Tufvesson, E. and Andersson, C. and Weidner, J. and Erjefält, J. S. and Bjermer, L.},
  issn         = {0105-4538},
  keyword      = {Asthma,Exhaled nitric oxide,Inducible nitric oxide synthase},
  language     = {eng},
  number       = {4},
  pages        = {627--635},
  publisher    = {Wiley-Blackwell},
  series       = {Allergy: European Journal of Allergy and Clinical Immunology},
  title        = {Inducible nitric oxide synthase expression is increased in the alveolar compartment of asthmatic patients},
  url          = {http://dx.doi.org/10.1111/all.13052},
  volume       = {72},
  year         = {2017},
}