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Fine-tuning the structure of glycosaminoglycans in living cells using xylosides

Persson, Andrea LU ; Ellervik, Ulf LU and Mani, Katrin LU (2018) In Glycobiology 28(7). p.499-511
Abstract

Xylosides can induce the formation and secretion of xyloside-primed glycosaminoglycans when administered to living cells; however, their impact on the detailed glycosaminoglycan structure remains unknown. Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition. We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate. The disaccharide... (More)

Xylosides can induce the formation and secretion of xyloside-primed glycosaminoglycans when administered to living cells; however, their impact on the detailed glycosaminoglycan structure remains unknown. Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition. We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate. The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside. Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Chondroitin sulfate, dermatan sulfate, glycosaminoglycan, heparan sulfate, xyloside
in
Glycobiology
volume
28
issue
7
pages
13 pages
publisher
Oxford University Press
external identifiers
  • scopus:85049060228
ISSN
0959-6658
DOI
10.1093/glycob/cwy049
language
English
LU publication?
yes
id
ed473689-7c4d-4c6b-8dbb-b4d9d9c47cbf
date added to LUP
2018-07-09 11:42:32
date last changed
2018-09-09 05:00:49
@article{ed473689-7c4d-4c6b-8dbb-b4d9d9c47cbf,
  abstract     = {<p>Xylosides can induce the formation and secretion of xyloside-primed glycosaminoglycans when administered to living cells; however, their impact on the detailed glycosaminoglycan structure remains unknown. Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition. We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate. The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside. Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.</p>},
  author       = {Persson, Andrea and Ellervik, Ulf and Mani, Katrin},
  issn         = {0959-6658},
  keyword      = {Chondroitin sulfate,dermatan sulfate,glycosaminoglycan,heparan sulfate,xyloside},
  language     = {eng},
  month        = {07},
  number       = {7},
  pages        = {499--511},
  publisher    = {Oxford University Press},
  series       = {Glycobiology},
  title        = {Fine-tuning the structure of glycosaminoglycans in living cells using xylosides},
  url          = {http://dx.doi.org/10.1093/glycob/cwy049},
  volume       = {28},
  year         = {2018},
}