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Development of the thermotolerant methylotrophic yeast hansenula polymorpha as efficient ethanol producer

Sibirny, Andriy; Dmytruk, Kostyantyn; Kurylenko, Olena; Ruchala, Justyna and Ishchuk, Olena LU (2017) In Yeast Diversity in Human Welfare p.257-282
Abstract

Until recently, the methylotrophic yeasts, including Hansenula polymorpha, have not been considered as a potential producer of biofuels, particularly, ethanol from lignocellulosics. However it is already known that the thermotolerant methylotrophic yeast H. polymorpha is capable to ferment xylose, glucose and cellobiose, the main sugars of lignocellulosic hydrolysates, under elevated temperature. These observations allow considering H. polymorpha as a promising organism for high temperature alcoholic fermentation in industrial applications. Although the amount of ethanol produced from xylose by the wild-type strains of H. polymorpha is extremely low, the successful approaches of metabolic engineering and classical selection had been... (More)

Until recently, the methylotrophic yeasts, including Hansenula polymorpha, have not been considered as a potential producer of biofuels, particularly, ethanol from lignocellulosics. However it is already known that the thermotolerant methylotrophic yeast H. polymorpha is capable to ferment xylose, glucose and cellobiose, the main sugars of lignocellulosic hydrolysates, under elevated temperature. These observations allow considering H. polymorpha as a promising organism for high temperature alcoholic fermentation in industrial applications. Although the amount of ethanol produced from xylose by the wild-type strains of H. polymorpha is extremely low, the successful approaches of metabolic engineering and classical selection had been developed during last decade, which permitted to increase ethanol accumulation from xylose 30-fold. The available strains accumulate 12.5 g of ethanol per liter from xylose at 45 °C. In this article, we present published and new approaches and main achievements on metabolic engineering and selection of H. polymorpha for improved producers of ethanol from xylose, starch, xylan, and glycerol, as well as that of strains with increased tolerance to high temperatures and ethanol.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Ethanol, H. polymorpha, Metabolic engineering, Methylotrophic yeasts, Yeasts
in
Yeast Diversity in Human Welfare
pages
26 pages
publisher
Springer Singapore
external identifiers
  • scopus:85034006596
ISBN
9789811026201
9789811026218
DOI
10.1007/978-981-10-2621-8_11
language
English
LU publication?
yes
id
ed51a2d9-43ec-44ad-b6a6-8c9f164d6754
date added to LUP
2017-12-19 13:39:48
date last changed
2018-01-07 12:29:22
@inbook{ed51a2d9-43ec-44ad-b6a6-8c9f164d6754,
  abstract     = {<p>Until recently, the methylotrophic yeasts, including Hansenula polymorpha, have not been considered as a potential producer of biofuels, particularly, ethanol from lignocellulosics. However it is already known that the thermotolerant methylotrophic yeast H. polymorpha is capable to ferment xylose, glucose and cellobiose, the main sugars of lignocellulosic hydrolysates, under elevated temperature. These observations allow considering H. polymorpha as a promising organism for high temperature alcoholic fermentation in industrial applications. Although the amount of ethanol produced from xylose by the wild-type strains of H. polymorpha is extremely low, the successful approaches of metabolic engineering and classical selection had been developed during last decade, which permitted to increase ethanol accumulation from xylose 30-fold. The available strains accumulate 12.5 g of ethanol per liter from xylose at 45 °C. In this article, we present published and new approaches and main achievements on metabolic engineering and selection of H. polymorpha for improved producers of ethanol from xylose, starch, xylan, and glycerol, as well as that of strains with increased tolerance to high temperatures and ethanol.</p>},
  author       = {Sibirny, Andriy and Dmytruk, Kostyantyn and Kurylenko, Olena and Ruchala, Justyna and Ishchuk, Olena},
  isbn         = {9789811026201},
  keyword      = {Ethanol,H. polymorpha,Metabolic engineering,Methylotrophic yeasts,Yeasts},
  language     = {eng},
  month        = {05},
  pages        = {257--282},
  publisher    = {Springer Singapore},
  series       = {Yeast Diversity in Human Welfare},
  title        = {Development of the thermotolerant methylotrophic yeast hansenula polymorpha as efficient ethanol producer},
  url          = {http://dx.doi.org/10.1007/978-981-10-2621-8_11},
  year         = {2017},
}