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AMPKβ isoform expression patterns in various adipocyte models and in relation to body mass index

Kopietz, Franziska LU orcid ; Degerman, Eva LU orcid and Göransson, Olga LU orcid (2022) In Frontiers in Physiology 13.
Abstract

AMP-activated protein kinase (AMPK) activation is considered a useful strategy for the treatment of type 2 diabetes (T2D). It is unclear whether the expression and/or activity of AMPK in adipocytes is dysregulated in obesity. Also, the expression/activity pattern of AMPKβ isoforms, which are targets for AMPK activators, in adipocytes remains elusive. In this study we show that the two AMPKβ isoforms make roughly equal contributions to AMPK activity in primary human and mouse adipocytes, whereas in cultured 3T3-L1 adipocytes of mouse origin and in primary rat adipocytes, β1-associated activity clearly dominates. Additionally, we found that obesity is not associated with changes in AMPK subunit expression or kinase activity in adipocytes... (More)

AMP-activated protein kinase (AMPK) activation is considered a useful strategy for the treatment of type 2 diabetes (T2D). It is unclear whether the expression and/or activity of AMPK in adipocytes is dysregulated in obesity. Also, the expression/activity pattern of AMPKβ isoforms, which are targets for AMPK activators, in adipocytes remains elusive. In this study we show that the two AMPKβ isoforms make roughly equal contributions to AMPK activity in primary human and mouse adipocytes, whereas in cultured 3T3-L1 adipocytes of mouse origin and in primary rat adipocytes, β1-associated activity clearly dominates. Additionally, we found that obesity is not associated with changes in AMPK subunit expression or kinase activity in adipocytes isolated from subcutaneous adipose tissue from individuals with various BMI.

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Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adipocytes, AMPKβ, expression, human, kinase activity, obesity
in
Frontiers in Physiology
volume
13
article number
928964
publisher
Frontiers Media S. A.
external identifiers
  • scopus:85136460616
  • pmid:35991175
ISSN
1664-042X
DOI
10.3389/fphys.2022.928964
language
English
LU publication?
yes
id
edbc410c-a121-4ee2-a45b-6c5ce8a7ca74
date added to LUP
2023-01-03 16:26:59
date last changed
2024-04-18 11:30:45
@article{edbc410c-a121-4ee2-a45b-6c5ce8a7ca74,
  abstract     = {{<p>AMP-activated protein kinase (AMPK) activation is considered a useful strategy for the treatment of type 2 diabetes (T2D). It is unclear whether the expression and/or activity of AMPK in adipocytes is dysregulated in obesity. Also, the expression/activity pattern of AMPKβ isoforms, which are targets for AMPK activators, in adipocytes remains elusive. In this study we show that the two AMPKβ isoforms make roughly equal contributions to AMPK activity in primary human and mouse adipocytes, whereas in cultured 3T3-L1 adipocytes of mouse origin and in primary rat adipocytes, β1-associated activity clearly dominates. Additionally, we found that obesity is not associated with changes in AMPK subunit expression or kinase activity in adipocytes isolated from subcutaneous adipose tissue from individuals with various BMI.</p>}},
  author       = {{Kopietz, Franziska and Degerman, Eva and Göransson, Olga}},
  issn         = {{1664-042X}},
  keywords     = {{adipocytes; AMPKβ; expression; human; kinase activity; obesity}},
  language     = {{eng}},
  month        = {{08}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Physiology}},
  title        = {{AMPKβ isoform expression patterns in various adipocyte models and in relation to body mass index}},
  url          = {{http://dx.doi.org/10.3389/fphys.2022.928964}},
  doi          = {{10.3389/fphys.2022.928964}},
  volume       = {{13}},
  year         = {{2022}},
}