Chromatin interaction-based annotation of regulatory elements reveals dynamic promoter-enhancer interactions in lymphocyte development
Tingvall-Gustafsson, Johanna LU
; Jensen, Christina T.
LU
; Ungerbäck, Jonas
LU
and Sigvardsson, Mikael
LU
(2025)
In iScience
28(7).
- Abstract
Stage- and lineage-specific gene expression patterns are controlled by a complex interplay between transcription factors, the epigenetic landscape, and the three-dimensional (3D) structure of the DNA. The 3D structure allows for the formation of DNA loops that juxtaposition distal regulatory elements to the promoters, allowing for tight control of gene expression. Developing a tool to facilitate the exploration of complex gene regulatory networks based on chromosome configuration data in early lymphocytes, we show that lineage-specific transcription factors target regulatory elements annotated to both lineage-specific and broadly expressed genes. Several regulatory elements annotated to lineage-specific genes were also annotated to... (More)
Stage- and lineage-specific gene expression patterns are controlled by a complex interplay between transcription factors, the epigenetic landscape, and the three-dimensional (3D) structure of the DNA. The 3D structure allows for the formation of DNA loops that juxtaposition distal regulatory elements to the promoters, allowing for tight control of gene expression. Developing a tool to facilitate the exploration of complex gene regulatory networks based on chromosome configuration data in early lymphocytes, we show that lineage-specific transcription factors target regulatory elements annotated to both lineage-specific and broadly expressed genes. Several regulatory elements annotated to lineage-specific genes were also annotated to alternative promoters in a context-dependent manner, revealing a highly complex interplay between promoters and DREs in early lymphocyte development. These data highlight how efficient annotation procedures for linking distal regulatory elements to target genes provide valuable insights into gene regulatory networks.
(Less)
- author
- Tingvall-Gustafsson, Johanna
LU
; Jensen, Christina T.
LU
; Ungerbäck, Jonas
LU
and Sigvardsson, Mikael
LU
- organization
- publishing date
- 2025-07-18
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Chromosome organization, Components of the immune system, Epigenetics, Molecular genetics
- in
- iScience
- volume
- 28
- issue
- 7
- article number
- 112855
- publisher
- Elsevier
- external identifiers
-
- scopus:105010094429
- pmid:40687796
- ISSN
- 2589-0042
- DOI
- 10.1016/j.isci.2025.112855
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2025 The Author(s)
- id
- edf8fba1-63b0-490f-8ecf-9a940468e712
- date added to LUP
- 2025-12-05 15:34:23
- date last changed
- 2025-12-06 03:00:02
@article{edf8fba1-63b0-490f-8ecf-9a940468e712,
abstract = {{<p>Stage- and lineage-specific gene expression patterns are controlled by a complex interplay between transcription factors, the epigenetic landscape, and the three-dimensional (3D) structure of the DNA. The 3D structure allows for the formation of DNA loops that juxtaposition distal regulatory elements to the promoters, allowing for tight control of gene expression. Developing a tool to facilitate the exploration of complex gene regulatory networks based on chromosome configuration data in early lymphocytes, we show that lineage-specific transcription factors target regulatory elements annotated to both lineage-specific and broadly expressed genes. Several regulatory elements annotated to lineage-specific genes were also annotated to alternative promoters in a context-dependent manner, revealing a highly complex interplay between promoters and DREs in early lymphocyte development. These data highlight how efficient annotation procedures for linking distal regulatory elements to target genes provide valuable insights into gene regulatory networks.</p>}},
author = {{Tingvall-Gustafsson, Johanna and Jensen, Christina T. and Ungerbäck, Jonas and Sigvardsson, Mikael}},
issn = {{2589-0042}},
keywords = {{Chromosome organization; Components of the immune system; Epigenetics; Molecular genetics}},
language = {{eng}},
month = {{07}},
number = {{7}},
publisher = {{Elsevier}},
series = {{iScience}},
title = {{Chromatin interaction-based annotation of regulatory elements reveals dynamic promoter-enhancer interactions in lymphocyte development}},
url = {{http://dx.doi.org/10.1016/j.isci.2025.112855}},
doi = {{10.1016/j.isci.2025.112855}},
volume = {{28}},
year = {{2025}},
}