Polarization Portraits of Single Multichromophoric Systems: Visualizing Conformation and Energy Transfer.
Mirzov, Oleg LU ; Bloem, Robbert LU ; Hania, Ralph LU ; Thomsson, Daniel LU ; Lin, Hongzhen LU and Scheblykin, Ivan LU
(2009)
In Small
5.
p.1877-1888
- Abstract
- A novel technique, two-dimensional (2D) polarization single-molecule imaging, is presented. It is based on measurements and analysis of fluorescence intensity as a function of excitation and emission polarization angles. The technique allows recording of full information on the steady-state polarization properties of fluorescent objects. It is particularly suitable for application to single multichromophoric systems (molecules or nanoparticles) with energy transfer (ET) between different chromophores (e.g., single fluorescent pi-conjugated polymer chains). The 2D polarization data simultaneously provide information on the conformation of the system and the efficiency of its internal excitation ET. The technique is used to characterize... (More)
- A novel technique, two-dimensional (2D) polarization single-molecule imaging, is presented. It is based on measurements and analysis of fluorescence intensity as a function of excitation and emission polarization angles. The technique allows recording of full information on the steady-state polarization properties of fluorescent objects. It is particularly suitable for application to single multichromophoric systems (molecules or nanoparticles) with energy transfer (ET) between different chromophores (e.g., single fluorescent pi-conjugated polymer chains). The 2D polarization data simultaneously provide information on the conformation of the system and the efficiency of its internal excitation ET. The technique is used to characterize single chains and different kinds of chain aggregates of different conjugated polymers at different temperatures. The 2D polarization measurements reveal a dramatic difference in ET taking place in these systems. Clear temperature dependence of ET is observed for individual aggregates as well as for their statistical ensembles. Also, a dependence on solvent and aggregate size is shown. Additionally, extensive "traditional one-dimensional" polarization results on the polarization anisotropy of fluorescence excitation and emission are presented. These results and findings are discussed in relation to internal organization of the nano-objects under study. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1391919
- author
- Mirzov, Oleg
LU
; Bloem, Robbert
LU
; Hania, Ralph
LU
; Thomsson, Daniel
LU
; Lin, Hongzhen
LU
and Scheblykin, Ivan
LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Small
- volume
- 5
- pages
- 1877 - 1888
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000269227800012
- pmid:19384881
- scopus:69049095136
- ISSN
- 1613-6829
- DOI
- 10.1002/smll.200801168
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Chemical Physics (S) (011001060)
- id
- ee14dcb4-a995-4cc4-8cf4-81a37c2abc98 (old id 1391919)
- date added to LUP
- 2016-04-01 12:03:14
- date last changed
- 2022-03-28 19:34:14
@article{ee14dcb4-a995-4cc4-8cf4-81a37c2abc98,
abstract = {{A novel technique, two-dimensional (2D) polarization single-molecule imaging, is presented. It is based on measurements and analysis of fluorescence intensity as a function of excitation and emission polarization angles. The technique allows recording of full information on the steady-state polarization properties of fluorescent objects. It is particularly suitable for application to single multichromophoric systems (molecules or nanoparticles) with energy transfer (ET) between different chromophores (e.g., single fluorescent pi-conjugated polymer chains). The 2D polarization data simultaneously provide information on the conformation of the system and the efficiency of its internal excitation ET. The technique is used to characterize single chains and different kinds of chain aggregates of different conjugated polymers at different temperatures. The 2D polarization measurements reveal a dramatic difference in ET taking place in these systems. Clear temperature dependence of ET is observed for individual aggregates as well as for their statistical ensembles. Also, a dependence on solvent and aggregate size is shown. Additionally, extensive "traditional one-dimensional" polarization results on the polarization anisotropy of fluorescence excitation and emission are presented. These results and findings are discussed in relation to internal organization of the nano-objects under study.}},
author = {{Mirzov, Oleg and Bloem, Robbert and Hania, Ralph and Thomsson, Daniel and Lin, Hongzhen and Scheblykin, Ivan}},
issn = {{1613-6829}},
language = {{eng}},
pages = {{1877--1888}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Small}},
title = {{Polarization Portraits of Single Multichromophoric Systems: Visualizing Conformation and Energy Transfer.}},
url = {{http://dx.doi.org/10.1002/smll.200801168}},
doi = {{10.1002/smll.200801168}},
volume = {{5}},
year = {{2009}},
}