Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Bottom-up cubosome synthesis without organic solvents

Bryant, Saffron J. ; Bathke, Elly K. LU and Edler, Karen J. LU orcid (2021) In Journal of Colloid and Interface Science 601. p.98-105
Abstract

Hypothesis: Bottom-up synthesis of cubosomes is more energetically favourable than top-down approaches. However, bottom-up methods often rely on organic solvents such as ethanol as diluents, and lead to concurrent formation of liposomes. We propose using non-toxic diluents such as honey, glycerol and lactic acid for bottom-up cubosome synthesis.

Experiments: Cubosomes were prepared using solutions of phytantriol in a range of diluents including choline chloride-glycerol, honey, lactic acid, glycerol, and ethanol. These solutions were added dropwise to water containing the stabiliser, poloxamer 407, following an established method of cubosome synthesis.

The resulting structures were characterised using small-angle... (More)

Hypothesis: Bottom-up synthesis of cubosomes is more energetically favourable than top-down approaches. However, bottom-up methods often rely on organic solvents such as ethanol as diluents, and lead to concurrent formation of liposomes. We propose using non-toxic diluents such as honey, glycerol and lactic acid for bottom-up cubosome synthesis.

Experiments: Cubosomes were prepared using solutions of phytantriol in a range of diluents including choline chloride-glycerol, honey, lactic acid, glycerol, and ethanol. These solutions were added dropwise to water containing the stabiliser, poloxamer 407, following an established method of cubosome synthesis.

The resulting structures were characterised using small-angle X-ray scattering, DLS and cryo-TEM.

Findings: Cubosomes were successfully formed using a range of non-toxic diluents. This demonstrates that harmful organic solvents like ethanol are not required, and that the diluents need not be hydrotropes. Furthermore, unlike ethanol, these other diluents allowed formation of cubosomes without concurrent formation of liposomes. Given the huge potential for cubosomes in drug delivery, this new method offers a potentially useful low-cost, low-toxicity synthesis option.

(Less)
Please use this url to cite or link to this publication:
author
; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cubic phase, Cubosomes, Deep eutectic solvent, Drug delivery, Liquid crystal phase, Phytantriol
in
Journal of Colloid and Interface Science
volume
601
pages
8 pages
publisher
Elsevier
external identifiers
  • pmid:34058556
  • scopus:85107021549
ISSN
0021-9797
DOI
10.1016/j.jcis.2021.05.072
language
English
LU publication?
no
additional info
Publisher Copyright: © 2021 Elsevier Inc.
id
ee54d6be-84b4-46e4-b95f-246e00aa33a4
date added to LUP
2022-07-12 15:38:32
date last changed
2024-07-09 14:16:36
@article{ee54d6be-84b4-46e4-b95f-246e00aa33a4,
  abstract     = {{<p>Hypothesis: Bottom-up synthesis of cubosomes is more energetically favourable than top-down approaches. However, bottom-up methods often rely on organic solvents such as ethanol as diluents, and lead to concurrent formation of liposomes. We propose using non-toxic diluents such as honey, glycerol and lactic acid for bottom-up cubosome synthesis. <br/></p><p>Experiments: Cubosomes were prepared using solutions of phytantriol in a range of diluents including choline chloride-glycerol, honey, lactic acid, glycerol, and ethanol. These solutions were added dropwise to water containing the stabiliser, poloxamer 407, following an established method of cubosome synthesis. <br/></p><p>The resulting structures were characterised using small-angle X-ray scattering, DLS and cryo-TEM. <br/></p><p>Findings: Cubosomes were successfully formed using a range of non-toxic diluents. This demonstrates that harmful organic solvents like ethanol are not required, and that the diluents need not be hydrotropes. Furthermore, unlike ethanol, these other diluents allowed formation of cubosomes without concurrent formation of liposomes. Given the huge potential for cubosomes in drug delivery, this new method offers a potentially useful low-cost, low-toxicity synthesis option.</p>}},
  author       = {{Bryant, Saffron J. and Bathke, Elly K. and Edler, Karen J.}},
  issn         = {{0021-9797}},
  keywords     = {{Cubic phase; Cubosomes; Deep eutectic solvent; Drug delivery; Liquid crystal phase; Phytantriol}},
  language     = {{eng}},
  pages        = {{98--105}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Colloid and Interface Science}},
  title        = {{Bottom-up cubosome synthesis without organic solvents}},
  url          = {{http://dx.doi.org/10.1016/j.jcis.2021.05.072}},
  doi          = {{10.1016/j.jcis.2021.05.072}},
  volume       = {{601}},
  year         = {{2021}},
}