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Immune phenotypes of nasopharyngeal cancer

Nilsson, Johan S. LU ; Sobti, Aastha LU ; Swoboda, Sabine LU orcid ; Erjefält, Jonas S. LU ; Forslund, Ola LU ; Lindstedt, Malin LU and Greiff, Lennart LU (2020) In Cancers 12(11). p.1-17
Abstract

Nasopharyngeal cancer (NPC) features intralesional immune cells, but data are lacking on presence/distribution of T-cells and dendritic cells (DCs). Based on intralesional distribution of lymphocytes, a series of NPC biopsies (n = 48) were classified into “inflamed”, “excluded”, and “deserted” phenotypes. In addition, CD8+ T-cells and CD207+ DCs were quantified. The data were analyzed in relation to Epstein–Barr virus-encoded small RNA (EBER), Epstein-Barr virus (EBV) DNA, and survival. Separately, data on gene expression from a public database were analyzed. 61.7% of NPC lesions were “inflamed”, 29.8% were “excluded”, and 8.5% were “deserted”. While CD8+ cells were present in cancer cell areas and in surrounding stroma, CD207+ cells... (More)

Nasopharyngeal cancer (NPC) features intralesional immune cells, but data are lacking on presence/distribution of T-cells and dendritic cells (DCs). Based on intralesional distribution of lymphocytes, a series of NPC biopsies (n = 48) were classified into “inflamed”, “excluded”, and “deserted” phenotypes. In addition, CD8+ T-cells and CD207+ DCs were quantified. The data were analyzed in relation to Epstein–Barr virus-encoded small RNA (EBER), Epstein-Barr virus (EBV) DNA, and survival. Separately, data on gene expression from a public database were analyzed. 61.7% of NPC lesions were “inflamed”, 29.8% were “excluded”, and 8.5% were “deserted”. While CD8+ cells were present in cancer cell areas and in surrounding stroma, CD207+ cells were observed largely in cancer cell areas. High CD8+ T-cell presence was associated with EBV+ disease, but no such pattern was observed for CD207+ DCs. There was a difference in disease-free survival in favor of “inflamed” over “excluded” NPC. Gene expression analysis revealed differences between NPC and control tissue (e.g., with regard to interferon activity) as well as between subgroups of NPC based on CD8 expression (high vs. low). In conclusion, NPC lesions are heterogeneous with regard to distribution of CD8+ T-cells and CD207+ DCs. NPC can be classified into immune phenotypes that carry prognostic information. CD207+ DCs may represent a target for immunotherapy with potential to facilitate the antigen cross-presentation necessary to execute cytotoxic T-lymphocyte responses.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cancer immune phenotype, CD207, CD8, Quantification, Survival
in
Cancers
volume
12
issue
11
article number
3428
pages
17 pages
publisher
MDPI AG
external identifiers
  • scopus:85096232482
  • pmid:33218184
ISSN
2072-6694
DOI
10.3390/cancers12113428
language
English
LU publication?
yes
id
ee6fa486-cf5e-4a0f-8099-16b38b441d36
date added to LUP
2021-01-14 16:10:15
date last changed
2024-06-28 09:27:10
@article{ee6fa486-cf5e-4a0f-8099-16b38b441d36,
  abstract     = {{<p>Nasopharyngeal cancer (NPC) features intralesional immune cells, but data are lacking on presence/distribution of T-cells and dendritic cells (DCs). Based on intralesional distribution of lymphocytes, a series of NPC biopsies (n = 48) were classified into “inflamed”, “excluded”, and “deserted” phenotypes. In addition, CD8+ T-cells and CD207+ DCs were quantified. The data were analyzed in relation to Epstein–Barr virus-encoded small RNA (EBER), Epstein-Barr virus (EBV) DNA, and survival. Separately, data on gene expression from a public database were analyzed. 61.7% of NPC lesions were “inflamed”, 29.8% were “excluded”, and 8.5% were “deserted”. While CD8+ cells were present in cancer cell areas and in surrounding stroma, CD207+ cells were observed largely in cancer cell areas. High CD8+ T-cell presence was associated with EBV+ disease, but no such pattern was observed for CD207+ DCs. There was a difference in disease-free survival in favor of “inflamed” over “excluded” NPC. Gene expression analysis revealed differences between NPC and control tissue (e.g., with regard to interferon activity) as well as between subgroups of NPC based on CD8 expression (high vs. low). In conclusion, NPC lesions are heterogeneous with regard to distribution of CD8+ T-cells and CD207+ DCs. NPC can be classified into immune phenotypes that carry prognostic information. CD207+ DCs may represent a target for immunotherapy with potential to facilitate the antigen cross-presentation necessary to execute cytotoxic T-lymphocyte responses.</p>}},
  author       = {{Nilsson, Johan S. and Sobti, Aastha and Swoboda, Sabine and Erjefält, Jonas S. and Forslund, Ola and Lindstedt, Malin and Greiff, Lennart}},
  issn         = {{2072-6694}},
  keywords     = {{Cancer immune phenotype; CD207; CD8; Quantification; Survival}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{1--17}},
  publisher    = {{MDPI AG}},
  series       = {{Cancers}},
  title        = {{Immune phenotypes of nasopharyngeal cancer}},
  url          = {{http://dx.doi.org/10.3390/cancers12113428}},
  doi          = {{10.3390/cancers12113428}},
  volume       = {{12}},
  year         = {{2020}},
}