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Transcription factor AP-4 is a ligand for immunoglobulin-κ promoter E-box elements

Aranburu, A. ; Carlsson, R. LU ; Persson, C. and Leanderson, T. LU (2001) In Biochemical Journal 354(2). p.431-438
Abstract

Immunoglobulin (Ig)-κ promoters from humans and mice share conserved sequences. The octamer element is common to all Ig promoters and pivotal for their function. However, other conserved sequence motifs, that differ between lg variable gene families, are required for normal promoter function. These conserved motifs do not stimulate transcription in the absence of an octamer. One example is an E-box of the E47/E12 type (5′-CAGCTG-3′), which is found in all promoters of the human and murine Ig-κ gene subgroups/families, with the exception of subgroups II and VI and their related murine families. In the present study we show that the ubiquitously expressed transcription factor AP-4, and not E47, interacts specifically with the κ promoter... (More)

Immunoglobulin (Ig)-κ promoters from humans and mice share conserved sequences. The octamer element is common to all Ig promoters and pivotal for their function. However, other conserved sequence motifs, that differ between lg variable gene families, are required for normal promoter function. These conserved motifs do not stimulate transcription in the absence of an octamer. One example is an E-box of the E47/E12 type (5′-CAGCTG-3′), which is found in all promoters of the human and murine Ig-κ gene subgroups/families, with the exception of subgroups II and VI and their related murine families. In the present study we show that the ubiquitously expressed transcription factor AP-4, and not E47, interacts specifically with the κ promoter E-boxes when tested in electrophoretic mobility-shift assays using nuclear extracts derived from human and murine B-cell lines. Furthermore, AP-4, unlike E47, did not act as a transactivator, which is in agreement with previous studies on intact κ promoters, showing that transcription is absent when the octamer element has been mutated. Based on these data, and the conservation of the 5′-CAGCTG-3′ motif among human and murine κ promoters, we propose that AP-4 is the major ligand for Ig-κ promoter E-boxes.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bHLH-Zip protein, Conserved sequences, Immunoglobulin genes
in
Biochemical Journal
volume
354
issue
2
pages
8 pages
publisher
Portland Press Limited
external identifiers
  • pmid:11171123
  • scopus:0035281602
ISSN
0264-6021
DOI
10.1042/0264-6021:3540431
language
English
LU publication?
yes
id
ee728800-782e-496a-aa08-4c208d662a8b
date added to LUP
2019-07-02 10:08:14
date last changed
2020-01-13 02:11:08
@article{ee728800-782e-496a-aa08-4c208d662a8b,
  abstract     = {<p>Immunoglobulin (Ig)-κ promoters from humans and mice share conserved sequences. The octamer element is common to all Ig promoters and pivotal for their function. However, other conserved sequence motifs, that differ between lg variable gene families, are required for normal promoter function. These conserved motifs do not stimulate transcription in the absence of an octamer. One example is an E-box of the E47/E12 type (5′-CAGCTG-3′), which is found in all promoters of the human and murine Ig-κ gene subgroups/families, with the exception of subgroups II and VI and their related murine families. In the present study we show that the ubiquitously expressed transcription factor AP-4, and not E47, interacts specifically with the κ promoter E-boxes when tested in electrophoretic mobility-shift assays using nuclear extracts derived from human and murine B-cell lines. Furthermore, AP-4, unlike E47, did not act as a transactivator, which is in agreement with previous studies on intact κ promoters, showing that transcription is absent when the octamer element has been mutated. Based on these data, and the conservation of the 5′-CAGCTG-3′ motif among human and murine κ promoters, we propose that AP-4 is the major ligand for Ig-κ promoter E-boxes.</p>},
  author       = {Aranburu, A. and Carlsson, R. and Persson, C. and Leanderson, T.},
  issn         = {0264-6021},
  language     = {eng},
  month        = {03},
  number       = {2},
  pages        = {431--438},
  publisher    = {Portland Press Limited},
  series       = {Biochemical Journal},
  title        = {Transcription factor AP-4 is a ligand for immunoglobulin-κ promoter E-box elements},
  url          = {http://dx.doi.org/10.1042/0264-6021:3540431},
  doi          = {10.1042/0264-6021:3540431},
  volume       = {354},
  year         = {2001},
}