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A Novel Capacitive Sensor Based on Molecularly Imprinted Nanoparticles as Recognition Elements

Canfarotta, Francesco; Czulak, Joanna ; Guerreiro, Antonio; Cruz, Alvaro Garcia; Piletsky, Sergey; Ertürk Bergdahl, Gizem LU ; Hedström, Martin LU and Mattiasson, Bo LU (2018) In Biosensors and Bioelectronics p.108-114
Abstract
Molecularly Imprinted Polymers (MIPs) are synthetic receptors capable of selective binding to their target (template) molecules and, hence, are used as recognition elements in assays and sensors as a replacement for relatively unstable enzymes and antibodies. Herein, we describe a manufacturing-friendly protocol for integration of MIP nanoparticles (nanoMIPs) with a (label-free) capacitive sensor. The nanoMIPs were produced by solid-phase synthesis for two templates with different sizes and properties, including a small molecule tetrahydrocannabinol (THC) and a protein (trypsin). NanoMIPs were deposited on the surface of the sensor and the change in capacitance (ΔC) upon binding of the target was measured. The significant improvement in... (More)
Molecularly Imprinted Polymers (MIPs) are synthetic receptors capable of selective binding to their target (template) molecules and, hence, are used as recognition elements in assays and sensors as a replacement for relatively unstable enzymes and antibodies. Herein, we describe a manufacturing-friendly protocol for integration of MIP nanoparticles (nanoMIPs) with a (label-free) capacitive sensor. The nanoMIPs were produced by solid-phase synthesis for two templates with different sizes and properties, including a small molecule tetrahydrocannabinol (THC) and a protein (trypsin). NanoMIPs were deposited on the surface of the sensor and the change in capacitance (ΔC) upon binding of the target was measured. The significant improvement in the selectivity and limit of detection (one order of magnitude compared to previously used MIP microparticles) can be attributed to their increased surface-to-volume ratio and higher specificity of the nanoMIPs produced by the solid-phase method. The methodology described is also compatible with common sensor fabrication approaches, as opposed to methods involving in situ MIP polymerisation. The proposed sensor shows high selectivity, fast sensor response (45 min including injection, regeneration and re-equilibration with running buffer), and straightforward data analysis, which makes it viable for label-free monitoring in real-time. The set of targets assessed in this manuscript shows the general applicability of the biosensor platform. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biosensors and Bioelectronics
pages
108 - 114
publisher
Elsevier
external identifiers
  • scopus:85052529606
ISSN
1873-4235
DOI
10.1016/j.bios.2018.07.070
language
English
LU publication?
yes
id
eeb8b68f-1c51-4546-a892-2093c2c54927
date added to LUP
2018-08-13 10:55:49
date last changed
2019-04-10 04:11:53
@article{eeb8b68f-1c51-4546-a892-2093c2c54927,
  abstract     = {Molecularly Imprinted Polymers (MIPs) are synthetic receptors capable of selective binding to their target (template) molecules and, hence, are used as recognition elements in assays and sensors as a replacement for relatively unstable enzymes and antibodies. Herein, we describe a manufacturing-friendly protocol for integration of MIP nanoparticles (nanoMIPs) with a (label-free) capacitive sensor. The nanoMIPs were produced by solid-phase synthesis for two templates with different sizes and properties, including a small molecule tetrahydrocannabinol (THC) and a protein (trypsin). NanoMIPs were deposited on the surface of the sensor and the change in capacitance (ΔC) upon binding of the target was measured. The significant improvement in the selectivity and limit of detection (one order of magnitude compared to previously used MIP microparticles) can be attributed to their increased surface-to-volume ratio and higher specificity of the nanoMIPs produced by the solid-phase method. The methodology described is also compatible with common sensor fabrication approaches, as opposed to methods involving in situ MIP polymerisation. The proposed sensor shows high selectivity, fast sensor response (45 min including injection, regeneration and re-equilibration with running buffer), and straightforward data analysis, which makes it viable for label-free monitoring in real-time. The set of targets assessed in this manuscript shows the general applicability of the biosensor platform.},
  author       = {Canfarotta, Francesco and Czulak, Joanna  and Guerreiro, Antonio and Cruz, Alvaro Garcia and Piletsky, Sergey and Ertürk Bergdahl, Gizem and Hedström, Martin and Mattiasson, Bo},
  issn         = {1873-4235},
  language     = {eng},
  pages        = {108--114},
  publisher    = {Elsevier},
  series       = {Biosensors and Bioelectronics},
  title        = {A Novel Capacitive Sensor Based on Molecularly Imprinted Nanoparticles as Recognition Elements},
  url          = {http://dx.doi.org/10.1016/j.bios.2018.07.070},
  year         = {2018},
}