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Chemokines modulate glycan binding and the immunoregulatory activity of galectins

Sanjurjo, Lucía ; Schulkens, Iris A. ; Touarin, Pauline ; Heusschen, Roy ; Aanhane, Ed ; Castricum, Kitty C.M. ; De Gruijl, Tanja D. ; Nilsson, Ulf J. LU ; Leffler, Hakon LU and Griffioen, Arjan W. , et al. (2021) In Communications Biology 4(1).
Abstract

Galectins are versatile glycan-binding proteins involved in immunomodulation. Evidence suggests that galectins can control the immunoregulatory function of cytokines and chemokines through direct binding. Here, we report on an inverse mechanism in which chemokines control the immunomodulatory functions of galectins. We show the existence of several specific galectin-chemokine binding pairs, including galectin-1/CXCL4. NMR analyses show that CXCL4 binding induces changes in the galectin-1 carbohydrate binding site. Consequently, CXCL4 alters the glycan-binding affinity and specificity of galectin-1. Regarding immunomodulation, CXCL4 significantly increases the apoptotic activity of galectin-1 on activated CD8+ T cells, while... (More)

Galectins are versatile glycan-binding proteins involved in immunomodulation. Evidence suggests that galectins can control the immunoregulatory function of cytokines and chemokines through direct binding. Here, we report on an inverse mechanism in which chemokines control the immunomodulatory functions of galectins. We show the existence of several specific galectin-chemokine binding pairs, including galectin-1/CXCL4. NMR analyses show that CXCL4 binding induces changes in the galectin-1 carbohydrate binding site. Consequently, CXCL4 alters the glycan-binding affinity and specificity of galectin-1. Regarding immunomodulation, CXCL4 significantly increases the apoptotic activity of galectin-1 on activated CD8+ T cells, while no effect is observed in CD4+ T cells. The opposite is found for another galectin-chemokine pair, i.e., galectin-9/CCL5. This heterodimer significantly reduces the galectin-9 induced apoptosis of CD4+ T cells and not of CD8+ T cells. Collectively, the current study describes an immunomodulatory mechanism in which specific galectin-chemokine interactions control the glycan-binding activity and immunoregulatory function of galectins.

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publishing date
type
Contribution to journal
publication status
published
subject
in
Communications Biology
volume
4
issue
1
article number
1415
publisher
Nature Publishing Group
external identifiers
  • pmid:34931005
  • scopus:85121478643
ISSN
2399-3642
DOI
10.1038/s42003-021-02922-4
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2021, The Author(s).
id
eedf6984-f540-4312-9761-25f532f6e353
date added to LUP
2022-02-21 09:24:52
date last changed
2024-04-18 05:44:08
@article{eedf6984-f540-4312-9761-25f532f6e353,
  abstract     = {{<p>Galectins are versatile glycan-binding proteins involved in immunomodulation. Evidence suggests that galectins can control the immunoregulatory function of cytokines and chemokines through direct binding. Here, we report on an inverse mechanism in which chemokines control the immunomodulatory functions of galectins. We show the existence of several specific galectin-chemokine binding pairs, including galectin-1/CXCL4. NMR analyses show that CXCL4 binding induces changes in the galectin-1 carbohydrate binding site. Consequently, CXCL4 alters the glycan-binding affinity and specificity of galectin-1. Regarding immunomodulation, CXCL4 significantly increases the apoptotic activity of galectin-1 on activated CD8<sup>+</sup> T cells, while no effect is observed in CD4<sup>+</sup> T cells. The opposite is found for another galectin-chemokine pair, i.e., galectin-9/CCL5. This heterodimer significantly reduces the galectin-9 induced apoptosis of CD4<sup>+</sup> T cells and not of CD8<sup>+</sup> T cells. Collectively, the current study describes an immunomodulatory mechanism in which specific galectin-chemokine interactions control the glycan-binding activity and immunoregulatory function of galectins.</p>}},
  author       = {{Sanjurjo, Lucía and Schulkens, Iris A. and Touarin, Pauline and Heusschen, Roy and Aanhane, Ed and Castricum, Kitty C.M. and De Gruijl, Tanja D. and Nilsson, Ulf J. and Leffler, Hakon and Griffioen, Arjan W. and Elantak, Latifa and Koenen, Rory R. and Thijssen, Victor L.J.L.}},
  issn         = {{2399-3642}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Communications Biology}},
  title        = {{Chemokines modulate glycan binding and the immunoregulatory activity of galectins}},
  url          = {{http://dx.doi.org/10.1038/s42003-021-02922-4}},
  doi          = {{10.1038/s42003-021-02922-4}},
  volume       = {{4}},
  year         = {{2021}},
}