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Bacillus subtilis CcdA defective mutants are blocked in a late step of cytochrome c biogenesis

Schiött, Torbjörn LU ; Throne-Holst, Mimmi LU and Hederstedt, Lars LU (1997) In Journal of Bacteriology 179(14).
Abstract
Cytochromes of the c type contain covalently bound heme. In bacteria, they are located on the outside of the cytoplasmic membrane. Cytochrome c synthesis involves export of heme and apocytochrome across the cytoplasmic membrane followed by ligation of heme to the polypeptide. Using radioactive protoheme IX produced in Escherichia coli, we show that Bacillus subtilis can use heme from the growth medium for cytochrome c synthesis. The B. subtilis ccdA gene encodes a 26-kDa integral membrane protein which is required for cytochrome c synthesis (T. Schiott et al., J. Bacteriol. 179:1962-1973, 1997). In this work, we analyzed the stage at which cytochrome c synthesis is blocked in a ccdA deletion mutant. The following steps were found to be... (More)
Cytochromes of the c type contain covalently bound heme. In bacteria, they are located on the outside of the cytoplasmic membrane. Cytochrome c synthesis involves export of heme and apocytochrome across the cytoplasmic membrane followed by ligation of heme to the polypeptide. Using radioactive protoheme IX produced in Escherichia coli, we show that Bacillus subtilis can use heme from the growth medium for cytochrome c synthesis. The B. subtilis ccdA gene encodes a 26-kDa integral membrane protein which is required for cytochrome c synthesis (T. Schiott et al., J. Bacteriol. 179:1962-1973, 1997). In this work, we analyzed the stage at which cytochrome c synthesis is blocked in a ccdA deletion mutant. The following steps were found to be normal in the mutant: (i) transcription and translation of cytochrome c structural genes, (ii) translocation of apocytochrome across the cytoplasmic membrane, and (iii) heme transport from the cytoplasm to cytochrome polypeptide on the outer side of the cytoplasmic membrane. It is concluded that CcdA is required for a late step in the cytochrome c synthesis pathway (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
179
issue
14
publisher
American Society for Microbiology
external identifiers
  • scopus:0038228616
ISSN
0021-9193
DOI
10.1128/jb.179.14.4523-4529.1997
language
English
LU publication?
yes
id
eef3c5a8-5a44-45d2-8938-5bdfd9708fbf
date added to LUP
2017-07-18 10:01:14
date last changed
2022-01-30 21:32:22
@article{eef3c5a8-5a44-45d2-8938-5bdfd9708fbf,
  abstract     = {{Cytochromes of the c type contain covalently bound heme. In bacteria, they are located on the outside of the cytoplasmic membrane. Cytochrome c synthesis involves export of heme and apocytochrome across the cytoplasmic membrane followed by ligation of heme to the polypeptide. Using radioactive protoheme IX produced in Escherichia coli, we show that Bacillus subtilis can use heme from the growth medium for cytochrome c synthesis. The B. subtilis ccdA gene encodes a 26-kDa integral membrane protein which is required for cytochrome c synthesis (T. Schiott et al., J. Bacteriol. 179:1962-1973, 1997). In this work, we analyzed the stage at which cytochrome c synthesis is blocked in a ccdA deletion mutant. The following steps were found to be normal in the mutant: (i) transcription and translation of cytochrome c structural genes, (ii) translocation of apocytochrome across the cytoplasmic membrane, and (iii) heme transport from the cytoplasm to cytochrome polypeptide on the outer side of the cytoplasmic membrane. It is concluded that CcdA is required for a late step in the cytochrome c synthesis pathway}},
  author       = {{Schiött, Torbjörn and Throne-Holst, Mimmi and Hederstedt, Lars}},
  issn         = {{0021-9193}},
  language     = {{eng}},
  number       = {{14}},
  publisher    = {{American Society for Microbiology}},
  series       = {{Journal of Bacteriology}},
  title        = {{<em>Bacillus subtilis</em> CcdA defective mutants are blocked in a late step of cytochrome c biogenesis}},
  url          = {{http://dx.doi.org/10.1128/jb.179.14.4523-4529.1997}},
  doi          = {{10.1128/jb.179.14.4523-4529.1997}},
  volume       = {{179}},
  year         = {{1997}},
}