Disruption of β2-integrin-cytoskeleton coupling abolishes the signaling capacity of these integrins on granulocytes
(1999) In Biochemical and Biophysical Research Communications 265(1). p.164-169- Abstract
Integrin dependent adhesion and dynamic modulations of the actin network are prerequisites for normal cell locomotion. To investigate whether the actin microfilamentous system does play a role in regulation of β2-integrin-induced signalling, we pretreated granulocytes with staurosporine, a well-known protein kinase inhibitor that has also been shown to disrupt the cytoskeleton of intact cells. Pretreatment with staurosporine completely inhibited the β2-integrin-induced Ca2+ signal and also its ability to trigger actin polymerisation. This inhibition was not related to phosphorylation of the CD18-chain of the β2-integrin, nor to inhibition of protein kinases. Instead, association of... (More)
Integrin dependent adhesion and dynamic modulations of the actin network are prerequisites for normal cell locomotion. To investigate whether the actin microfilamentous system does play a role in regulation of β2-integrin-induced signalling, we pretreated granulocytes with staurosporine, a well-known protein kinase inhibitor that has also been shown to disrupt the cytoskeleton of intact cells. Pretreatment with staurosporine completely inhibited the β2-integrin-induced Ca2+ signal and also its ability to trigger actin polymerisation. This inhibition was not related to phosphorylation of the CD18-chain of the β2-integrin, nor to inhibition of protein kinases. Instead, association of β2-integrins with the cortical cytoskeleton, which was observed in untreated cells, was abolished after exposure to staurosporine, indicating that β2-integrin signalling depends on integrin-cytoskeleton interaction. These results suggest not only that the actin network provides an adhesive link to the extracellular matrix and a driving force for the locomotory response, but also that it participates in regulation of β2-integrin signalling during granulocyte locomotion.
(Less)
- author
- Hellberg, Carina ; Ydrenius, Liselotte ; Axelsson, Lena LU and Andersson, Tommy LU
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemical and Biophysical Research Communications
- volume
- 265
- issue
- 1
- pages
- 6 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0033547461
- pmid:10548508
- ISSN
- 0006-291X
- DOI
- 10.1006/bbrc.1999.1645
- language
- English
- LU publication?
- yes
- id
- ef4d0691-8ebc-4663-885a-7fac1172727c
- date added to LUP
- 2017-03-10 13:02:18
- date last changed
- 2025-01-07 09:25:42
@article{ef4d0691-8ebc-4663-885a-7fac1172727c, abstract = {{<p>Integrin dependent adhesion and dynamic modulations of the actin network are prerequisites for normal cell locomotion. To investigate whether the actin microfilamentous system does play a role in regulation of β<sub>2</sub>-integrin-induced signalling, we pretreated granulocytes with staurosporine, a well-known protein kinase inhibitor that has also been shown to disrupt the cytoskeleton of intact cells. Pretreatment with staurosporine completely inhibited the β<sub>2</sub>-integrin-induced Ca<sup>2+</sup> signal and also its ability to trigger actin polymerisation. This inhibition was not related to phosphorylation of the CD18-chain of the β<sub>2</sub>-integrin, nor to inhibition of protein kinases. Instead, association of β<sub>2</sub>-integrins with the cortical cytoskeleton, which was observed in untreated cells, was abolished after exposure to staurosporine, indicating that β<sub>2</sub>-integrin signalling depends on integrin-cytoskeleton interaction. These results suggest not only that the actin network provides an adhesive link to the extracellular matrix and a driving force for the locomotory response, but also that it participates in regulation of β<sub>2</sub>-integrin signalling during granulocyte locomotion.</p>}}, author = {{Hellberg, Carina and Ydrenius, Liselotte and Axelsson, Lena and Andersson, Tommy}}, issn = {{0006-291X}}, language = {{eng}}, number = {{1}}, pages = {{164--169}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Disruption of β<sub>2</sub>-integrin-cytoskeleton coupling abolishes the signaling capacity of these integrins on granulocytes}}, url = {{http://dx.doi.org/10.1006/bbrc.1999.1645}}, doi = {{10.1006/bbrc.1999.1645}}, volume = {{265}}, year = {{1999}}, }