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Proton MR spectroscopy of wild-type and creatine kinase deficient mouse skeletal muscle : dipole-dipole coupling effects and post-mortem changes

in 't Zandt, H J LU ; Klomp, D W; Oerlemans, F; Wieringa, B; Hilbers, C W and Heerschap, Arend (2000) In Magnetic Resonance in Medicine 43(4). p.24-517
Abstract

Localized proton MR spectra of mouse skeletal muscle obtained at 7 T show dipole-dipole coupling effects for creatine and putative taurine resonances and for the lactate methine signal. These effects are independent of the presence of creatine kinase. The intensity of the methylene (1)H resonance of creatine is not different between wild-type and creatine kinase deficient mice, which have a lower phosphocreatine content. (1)H-MR spectra acquired post-mortem from wild-type mouse skeletal muscle parallel to B(0) show a linewidth decrease for the methyl resonance of creatine and a 20% signal intensity loss for its methylene peak concurrent with the total breakdown of phosphocreatine as observed by (31)P-MR spectroscopy. However, with the... (More)

Localized proton MR spectra of mouse skeletal muscle obtained at 7 T show dipole-dipole coupling effects for creatine and putative taurine resonances and for the lactate methine signal. These effects are independent of the presence of creatine kinase. The intensity of the methylene (1)H resonance of creatine is not different between wild-type and creatine kinase deficient mice, which have a lower phosphocreatine content. (1)H-MR spectra acquired post-mortem from wild-type mouse skeletal muscle parallel to B(0) show a linewidth decrease for the methyl resonance of creatine and a 20% signal intensity loss for its methylene peak concurrent with the total breakdown of phosphocreatine as observed by (31)P-MR spectroscopy. However, with the muscle at the magic angle no changes in the appearance and intensity of creatine (and taurine) resonances are observed. These results indicate that the changes observed for creatine resonances are related to altered dipolar couplings and that the intensity of the methylene peak does not necessarily reflect muscular phosphocreatine content.

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publishing date
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Contribution to journal
publication status
published
keywords
Animals, Automatic Data Processing, Creatine Kinase, Culture Techniques, Data Display, Hindlimb, Image Enhancement, Magnetic Resonance Spectroscopy, Mice, Mice, Inbred C57BL, Mice, Inbred Strains, Muscle, Skeletal, Postmortem Changes, Sensitivity and Specificity, Comparative Study, Journal Article
in
Magnetic Resonance in Medicine
volume
43
issue
4
pages
8 pages
publisher
Wiley Online Library
external identifiers
  • scopus:0342313712
ISSN
0740-3194
DOI
10.1002/(SICI)1522-2594(200004)43:4<517::AID-MRM5>3.0.CO;2-I
language
English
LU publication?
no
id
ef5fd01b-9fea-43af-9c68-13be757ec7b0
date added to LUP
2017-03-06 16:47:34
date last changed
2017-07-09 04:59:03
@article{ef5fd01b-9fea-43af-9c68-13be757ec7b0,
  abstract     = {<p>Localized proton MR spectra of mouse skeletal muscle obtained at 7 T show dipole-dipole coupling effects for creatine and putative taurine resonances and for the lactate methine signal. These effects are independent of the presence of creatine kinase. The intensity of the methylene (1)H resonance of creatine is not different between wild-type and creatine kinase deficient mice, which have a lower phosphocreatine content. (1)H-MR spectra acquired post-mortem from wild-type mouse skeletal muscle parallel to B(0) show a linewidth decrease for the methyl resonance of creatine and a 20% signal intensity loss for its methylene peak concurrent with the total breakdown of phosphocreatine as observed by (31)P-MR spectroscopy. However, with the muscle at the magic angle no changes in the appearance and intensity of creatine (and taurine) resonances are observed. These results indicate that the changes observed for creatine resonances are related to altered dipolar couplings and that the intensity of the methylene peak does not necessarily reflect muscular phosphocreatine content.</p>},
  author       = {in 't Zandt, H J and Klomp, D W and Oerlemans, F and Wieringa, B and Hilbers, C W and Heerschap, Arend},
  issn         = {0740-3194},
  keyword      = {Animals,Automatic Data Processing,Creatine Kinase,Culture Techniques,Data Display,Hindlimb,Image Enhancement,Magnetic Resonance Spectroscopy,Mice,Mice, Inbred C57BL,Mice, Inbred Strains,Muscle, Skeletal,Postmortem Changes,Sensitivity and Specificity,Comparative Study,Journal Article},
  language     = {eng},
  number       = {4},
  pages        = {24--517},
  publisher    = {Wiley Online Library},
  series       = {Magnetic Resonance in Medicine},
  title        = {Proton MR spectroscopy of wild-type and creatine kinase deficient mouse skeletal muscle : dipole-dipole coupling effects and post-mortem changes},
  url          = {http://dx.doi.org/10.1002/(SICI)1522-2594(200004)43:4<517::AID-MRM5>3.0.CO;2-I},
  volume       = {43},
  year         = {2000},
}