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Differential expression of miR-139, miR-486 and miR-21 in breast cancer patients sub-classified according to lymph node status

Rask, Lene; Balslev, Eva; Søkilde, Rolf LU ; Høgdall, Estrid; Flyger, Henrik; Eriksen, Jens and Litman, Thomas (2014) In Cellular oncology (Dordrecht) 37(3). p.27-215
Abstract

PURPOSE: Therapeutic decisions in breast cancer are increasingly guided by prognostic and predictive biomarkers. Non-protein-coding microRNAs (miRNAs) have recently been found to be deregulated in breast cancers and, in addition, to be correlated with several clinico-pathological features. One of the most consistently up-regulated miRNAs is miR-21. Here, we specifically searched for differentially expressed miRNAs in high-risk breast cancer patients as compared to low-risk breast cancer patients. In the same patients, we also compared miR-21 expression with the expression of its presumed target PTEN.

METHODS: Both microarray and RT-qPCR techniques were used to assess miRNA expression levels in lymph node-positive and -negative... (More)

PURPOSE: Therapeutic decisions in breast cancer are increasingly guided by prognostic and predictive biomarkers. Non-protein-coding microRNAs (miRNAs) have recently been found to be deregulated in breast cancers and, in addition, to be correlated with several clinico-pathological features. One of the most consistently up-regulated miRNAs is miR-21. Here, we specifically searched for differentially expressed miRNAs in high-risk breast cancer patients as compared to low-risk breast cancer patients. In the same patients, we also compared miR-21 expression with the expression of its presumed target PTEN.

METHODS: Both microarray and RT-qPCR techniques were used to assess miRNA expression levels in lymph node-positive and -negative human invasive ductal carcinoma tissues. Simultaneously, PTEN protein expression levels were assessed using immunohistochemistry.

RESULTS: miR-486-5p and miR-139-5p were found to be down-regulated in patients with lymph node metastases, whereas miR-21 was found to be up-regulated in patients with a positive lymph node status. miR-21 expression levels were found to significantly correlate with tumour size (r = 0.403, p = 0.009; Spearman's rank), whereas no relation was found between miR-21 and PTEN expression levels (Kruskal-Wallis test).

CONCLUSION: Down-regulation of miR-486-5p and miR-139-5p, in conjunction with up-regulation of miR-21, may represent a useful signature for the identification of high-risk breast cancer patients.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Aged, Blotting, Western, Breast Neoplasms, Down-Regulation, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Immunohistochemistry, Lymphatic Metastasis, MicroRNAs, Middle Aged, Oligonucleotide Array Sequence Analysis, PTEN Phosphohydrolase, Reverse Transcriptase Polymerase Chain Reaction, Risk Factors, Up-Regulation, Journal Article, Research Support, Non-U.S. Gov't
in
Cellular oncology (Dordrecht)
volume
37
issue
3
pages
13 pages
publisher
Springer Netherlands
external identifiers
  • scopus:84904756218
ISSN
2211-3428
DOI
10.1007/s13402-014-0176-6
language
English
LU publication?
no
id
effd1273-6f21-4605-ae6f-8500fd91c938
date added to LUP
2017-09-01 14:29:01
date last changed
2017-09-11 12:42:15
@article{effd1273-6f21-4605-ae6f-8500fd91c938,
  abstract     = {<p>PURPOSE: Therapeutic decisions in breast cancer are increasingly guided by prognostic and predictive biomarkers. Non-protein-coding microRNAs (miRNAs) have recently been found to be deregulated in breast cancers and, in addition, to be correlated with several clinico-pathological features. One of the most consistently up-regulated miRNAs is miR-21. Here, we specifically searched for differentially expressed miRNAs in high-risk breast cancer patients as compared to low-risk breast cancer patients. In the same patients, we also compared miR-21 expression with the expression of its presumed target PTEN.</p><p>METHODS: Both microarray and RT-qPCR techniques were used to assess miRNA expression levels in lymph node-positive and -negative human invasive ductal carcinoma tissues. Simultaneously, PTEN protein expression levels were assessed using immunohistochemistry.</p><p>RESULTS: miR-486-5p and miR-139-5p were found to be down-regulated in patients with lymph node metastases, whereas miR-21 was found to be up-regulated in patients with a positive lymph node status. miR-21 expression levels were found to significantly correlate with tumour size (r = 0.403, p = 0.009; Spearman's rank), whereas no relation was found between miR-21 and PTEN expression levels (Kruskal-Wallis test).</p><p>CONCLUSION: Down-regulation of miR-486-5p and miR-139-5p, in conjunction with up-regulation of miR-21, may represent a useful signature for the identification of high-risk breast cancer patients.</p>},
  author       = {Rask, Lene and Balslev, Eva and Søkilde, Rolf and Høgdall, Estrid and Flyger, Henrik and Eriksen, Jens and Litman, Thomas},
  issn         = {2211-3428},
  keyword      = {Aged,Blotting, Western,Breast Neoplasms,Down-Regulation,Female,Gene Expression Profiling,Gene Expression Regulation, Neoplastic,Humans,Immunohistochemistry,Lymphatic Metastasis,MicroRNAs,Middle Aged,Oligonucleotide Array Sequence Analysis,PTEN Phosphohydrolase,Reverse Transcriptase Polymerase Chain Reaction,Risk Factors,Up-Regulation,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {27--215},
  publisher    = {Springer Netherlands},
  series       = {Cellular oncology (Dordrecht)},
  title        = {Differential expression of miR-139, miR-486 and miR-21 in breast cancer patients sub-classified according to lymph node status},
  url          = {http://dx.doi.org/10.1007/s13402-014-0176-6},
  volume       = {37},
  year         = {2014},
}