CADEE : Computer-Aided Directed Evolution of Enzymes
Amrein, Beat Anton ; Steffen-Munsberg, Fabian ; Szeler, Ireneusz ; Purg, Miha ; Kulkarni, Yashraj and Kamerlin, Shina Caroline Lynn LU
(2017)
In IUCrJ
4(Pt 1).
p.50-64
- Abstract
The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to prepare and analyze in silico semi-automated directed evolution of enzymes. A pedagogical example of the application of CADEE to a real biological system is also presented in order to illustrate the CADEE workflow.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/f0578c64-25db-41da-9bd8-121f2f460528
- author
- Amrein, Beat Anton
; Steffen-Munsberg, Fabian
; Szeler, Ireneusz
; Purg, Miha
; Kulkarni, Yashraj
and Kamerlin, Shina Caroline Lynn
LU
- publishing date
- 2017-01-01
- type
- Contribution to journal
- publication status
- published
- in
- IUCrJ
- volume
- 4
- issue
- Pt 1
- pages
- 15 pages
- publisher
- International Union of Crystallography
- external identifiers
-
- pmid:28250941
- scopus:85009074702
- ISSN
- 2052-2525
- DOI
- 10.1107/S2052252516018017
- language
- English
- LU publication?
- no
- id
- f0578c64-25db-41da-9bd8-121f2f460528
- date added to LUP
- 2025-01-11 21:26:46
- date last changed
- 2025-06-15 16:05:31
@article{f0578c64-25db-41da-9bd8-121f2f460528,
abstract = {{<p>The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to prepare and analyze in silico semi-automated directed evolution of enzymes. A pedagogical example of the application of CADEE to a real biological system is also presented in order to illustrate the CADEE workflow.</p>}},
author = {{Amrein, Beat Anton and Steffen-Munsberg, Fabian and Szeler, Ireneusz and Purg, Miha and Kulkarni, Yashraj and Kamerlin, Shina Caroline Lynn}},
issn = {{2052-2525}},
language = {{eng}},
month = {{01}},
number = {{Pt 1}},
pages = {{50--64}},
publisher = {{International Union of Crystallography}},
series = {{IUCrJ}},
title = {{<i>CADEE</i> : Computer-Aided Directed Evolution of Enzymes}},
url = {{http://dx.doi.org/10.1107/S2052252516018017}},
doi = {{10.1107/S2052252516018017}},
volume = {{4}},
year = {{2017}},
}