Standardizing the freeze-thaw preparation of growth factors from platelet lysate
(2017) In Transfusion 57(4). p.1058-1065- Abstract
BACKGROUND: Over the past decades, the focus on the regenerative properties of platelets (PLTs) has intensified and many PLT-derived growth factors are readily used in medical settings. A general lack of standardization in the preparation of these growth factors remains, however, and this study therefore examines the dynamics of growth factors throughout the freeze-thaw procedure.
STUDY DESIGN AND METHODS: Plateletpheresis (PA) and PLT-poor plasma (PPP) samples were collected from 10 healthy donors. PA was lysed to produce PLT lysate (PL) for 1, 3, 5, 10, and 30 freeze-thaw cycles. The resulting growth factor and cytokine concentrations from PPP, PA, and PL of different cycles were analyzed and compared using enzyme-linked... (More)
BACKGROUND: Over the past decades, the focus on the regenerative properties of platelets (PLTs) has intensified and many PLT-derived growth factors are readily used in medical settings. A general lack of standardization in the preparation of these growth factors remains, however, and this study therefore examines the dynamics of growth factors throughout the freeze-thaw procedure.
STUDY DESIGN AND METHODS: Plateletpheresis (PA) and PLT-poor plasma (PPP) samples were collected from 10 healthy donors. PA was lysed to produce PLT lysate (PL) for 1, 3, 5, 10, and 30 freeze-thaw cycles. The resulting growth factor and cytokine concentrations from PPP, PA, and PL of different cycles were analyzed and compared using enzyme-linked immunosorbent assay and multiplex bead assays.
RESULTS: PL produced by the freeze-thaw procedure resulted in approximately four- to 10-fold enrichment of transforming growth factor-β1, epidermal growth factor, PLT-derived growth factor (PDGF)-AB/BB, PLT factor-4, and fibroblast growth factor-2. The increase in concentrations plateaued at Cycles 3 and 5 and in some cases declined with further cycles. The concentrations of insulin-like growth factor-1, hepatocyte growth factor, vascular endothelial growth factor, and bone morphogenetic protein-2 in PL were essentially comparable to those in PPP.
CONCLUSION: Using the freeze-thaw method, optimal preparation of PL with regard to the concentration of growth factors was achieved at Cycles 3 to 5. Based on our findings, the clinical significance of using a greater number of cycles is likely limited.
(Less)
- author
- Strandberg, Gabriel LU ; Sellberg, Felix ; Sommar, Pehr ; Ronaghi, Martin ; Lubenow, Norbert ; Knutson, Folke and Berglund, David
- publishing date
- 2017
- type
- Contribution to journal
- publication status
- published
- keywords
- Blood Platelets/metabolism, Blood Preservation, Cryopreservation, Humans, Intercellular Signaling Peptides and Proteins/metabolism
- in
- Transfusion
- volume
- 57
- issue
- 4
- pages
- 1058 - 1065
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:28182293
- scopus:85013498538
- ISSN
- 1537-2995
- DOI
- 10.1111/trf.13998
- language
- English
- LU publication?
- no
- additional info
- © 2016 AABB.
- id
- f0a88f6b-6918-4415-bc68-02038ab37070
- date added to LUP
- 2022-04-28 10:04:50
- date last changed
- 2024-06-04 16:38:03
@article{f0a88f6b-6918-4415-bc68-02038ab37070,
abstract = {{<p>BACKGROUND: Over the past decades, the focus on the regenerative properties of platelets (PLTs) has intensified and many PLT-derived growth factors are readily used in medical settings. A general lack of standardization in the preparation of these growth factors remains, however, and this study therefore examines the dynamics of growth factors throughout the freeze-thaw procedure.</p><p>STUDY DESIGN AND METHODS: Plateletpheresis (PA) and PLT-poor plasma (PPP) samples were collected from 10 healthy donors. PA was lysed to produce PLT lysate (PL) for 1, 3, 5, 10, and 30 freeze-thaw cycles. The resulting growth factor and cytokine concentrations from PPP, PA, and PL of different cycles were analyzed and compared using enzyme-linked immunosorbent assay and multiplex bead assays.</p><p>RESULTS: PL produced by the freeze-thaw procedure resulted in approximately four- to 10-fold enrichment of transforming growth factor-β1, epidermal growth factor, PLT-derived growth factor (PDGF)-AB/BB, PLT factor-4, and fibroblast growth factor-2. The increase in concentrations plateaued at Cycles 3 and 5 and in some cases declined with further cycles. The concentrations of insulin-like growth factor-1, hepatocyte growth factor, vascular endothelial growth factor, and bone morphogenetic protein-2 in PL were essentially comparable to those in PPP.</p><p>CONCLUSION: Using the freeze-thaw method, optimal preparation of PL with regard to the concentration of growth factors was achieved at Cycles 3 to 5. Based on our findings, the clinical significance of using a greater number of cycles is likely limited.</p>}},
author = {{Strandberg, Gabriel and Sellberg, Felix and Sommar, Pehr and Ronaghi, Martin and Lubenow, Norbert and Knutson, Folke and Berglund, David}},
issn = {{1537-2995}},
keywords = {{Blood Platelets/metabolism; Blood Preservation; Cryopreservation; Humans; Intercellular Signaling Peptides and Proteins/metabolism}},
language = {{eng}},
number = {{4}},
pages = {{1058--1065}},
publisher = {{Wiley-Blackwell}},
series = {{Transfusion}},
title = {{Standardizing the freeze-thaw preparation of growth factors from platelet lysate}},
url = {{http://dx.doi.org/10.1111/trf.13998}},
doi = {{10.1111/trf.13998}},
volume = {{57}},
year = {{2017}},
}