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Determining if an mRNA is a Substrate of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae

Johansson, Marcus J O LU (2017) In Methods in molecular biology (Clifton, N.J.) p.169-177
Abstract

Nonsense-mediated mRNA decay (NMD) is a conserved eukaryotic quality control mechanism which triggers decay of mRNAs harboring premature translation termination codons. In this chapter, I describe methods for monitoring the influence of NMD on mRNA abundance and decay rates in Saccharomyces cerevisiae. The descriptions include detailed methods for growing yeast cells, total RNA isolation, and Northern blotting. Although the chapter focuses on NMD, the methods can be easily adapted to assess the effect of other mRNA decay pathways.

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author
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
keywords
Blotting, Northern, Gene Expression Profiling/methods, Gene Expression Regulation, Fungal, Half-Life, Nonsense Mediated mRNA Decay, RNA, Fungal/genetics, RNA, Messenger/genetics, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins
host publication
Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
series title
Methods in molecular biology (Clifton, N.J.)
pages
169 - 177
external identifiers
  • scopus:84994908518
  • pmid:27832540
ISSN
1940-6029
ISBN
1-4939-6516-8
978-1-4939-6518-2
DOI
10.1007/978-1-4939-6518-2_13
language
English
LU publication?
no
id
f0ca43b7-8e62-4742-8af9-c3dd7f4d451b
date added to LUP
2024-03-05 16:27:41
date last changed
2024-03-26 12:30:09
@inbook{f0ca43b7-8e62-4742-8af9-c3dd7f4d451b,
  abstract     = {{<p>Nonsense-mediated mRNA decay (NMD) is a conserved eukaryotic quality control mechanism which triggers decay of mRNAs harboring premature translation termination codons. In this chapter, I describe methods for monitoring the influence of NMD on mRNA abundance and decay rates in Saccharomyces cerevisiae. The descriptions include detailed methods for growing yeast cells, total RNA isolation, and Northern blotting. Although the chapter focuses on NMD, the methods can be easily adapted to assess the effect of other mRNA decay pathways.</p>}},
  author       = {{Johansson, Marcus J O}},
  booktitle    = {{Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation}},
  isbn         = {{1-4939-6516-8}},
  issn         = {{1940-6029}},
  keywords     = {{Blotting, Northern; Gene Expression Profiling/methods; Gene Expression Regulation, Fungal; Half-Life; Nonsense Mediated mRNA Decay; RNA, Fungal/genetics; RNA, Messenger/genetics; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins}},
  language     = {{eng}},
  pages        = {{169--177}},
  series       = {{Methods in molecular biology (Clifton, N.J.)}},
  title        = {{Determining if an mRNA is a Substrate of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae}},
  url          = {{http://dx.doi.org/10.1007/978-1-4939-6518-2_13}},
  doi          = {{10.1007/978-1-4939-6518-2_13}},
  year         = {{2017}},
}