Determining if an mRNA is a Substrate of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae
(2017) In Methods in molecular biology (Clifton, N.J.) p.169-177- Abstract
Nonsense-mediated mRNA decay (NMD) is a conserved eukaryotic quality control mechanism which triggers decay of mRNAs harboring premature translation termination codons. In this chapter, I describe methods for monitoring the influence of NMD on mRNA abundance and decay rates in Saccharomyces cerevisiae. The descriptions include detailed methods for growing yeast cells, total RNA isolation, and Northern blotting. Although the chapter focuses on NMD, the methods can be easily adapted to assess the effect of other mRNA decay pathways.
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https://lup.lub.lu.se/record/f0ca43b7-8e62-4742-8af9-c3dd7f4d451b
- author
- Johansson, Marcus J O LU
- publishing date
- 2017
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- keywords
- Blotting, Northern, Gene Expression Profiling/methods, Gene Expression Regulation, Fungal, Half-Life, Nonsense Mediated mRNA Decay, RNA, Fungal/genetics, RNA, Messenger/genetics, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins
- host publication
- Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
- series title
- Methods in molecular biology (Clifton, N.J.)
- pages
- 169 - 177
- external identifiers
-
- scopus:84994908518
- pmid:27832540
- ISSN
- 1940-6029
- ISBN
- 1-4939-6516-8
- 978-1-4939-6518-2
- DOI
- 10.1007/978-1-4939-6518-2_13
- language
- English
- LU publication?
- no
- id
- f0ca43b7-8e62-4742-8af9-c3dd7f4d451b
- date added to LUP
- 2024-03-05 16:27:41
- date last changed
- 2024-03-26 12:30:09
@inbook{f0ca43b7-8e62-4742-8af9-c3dd7f4d451b, abstract = {{<p>Nonsense-mediated mRNA decay (NMD) is a conserved eukaryotic quality control mechanism which triggers decay of mRNAs harboring premature translation termination codons. In this chapter, I describe methods for monitoring the influence of NMD on mRNA abundance and decay rates in Saccharomyces cerevisiae. The descriptions include detailed methods for growing yeast cells, total RNA isolation, and Northern blotting. Although the chapter focuses on NMD, the methods can be easily adapted to assess the effect of other mRNA decay pathways.</p>}}, author = {{Johansson, Marcus J O}}, booktitle = {{Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation}}, isbn = {{1-4939-6516-8}}, issn = {{1940-6029}}, keywords = {{Blotting, Northern; Gene Expression Profiling/methods; Gene Expression Regulation, Fungal; Half-Life; Nonsense Mediated mRNA Decay; RNA, Fungal/genetics; RNA, Messenger/genetics; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins}}, language = {{eng}}, pages = {{169--177}}, series = {{Methods in molecular biology (Clifton, N.J.)}}, title = {{Determining if an mRNA is a Substrate of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae}}, url = {{http://dx.doi.org/10.1007/978-1-4939-6518-2_13}}, doi = {{10.1007/978-1-4939-6518-2_13}}, year = {{2017}}, }