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The mitochondrial protein import component, TRANSLOCASE OF THE INNER MEMBRANE17-1, plays a role in defining the timing of germination in Arabidopsis

Wang, Yan; Law, Simon R; Ivanova, Aneta; van Aken, Olivier LU ; Kubiszewski-Jakubiak, Szymon; Uggalla, Vindya; van der Merwe, Margaretha; Duncan, Owen; Narsai, Reena and Whelan, James, et al. (2014) In Plant Physiology 166(3). p.35-1420
Abstract

In Arabidopsis (Arabidopsis thaliana), small gene families encode multiple isoforms for many of the components of the mitochondrial protein import apparatus. There are three isoforms of the TRANSLOCASE OF THE INNER MEMBRANE17 (Tim17). Transcriptome analysis indicates that AtTim17-1 is only detectable in dry seed. In this study, two independent transfer DNA insertional mutant lines of tim17-1 exhibited a germination-specific phenotype, showing a significant increase in the rate of germination. Microarray analyses revealed that Attim17-1 displayed alterations in the temporal sequence of transcriptomic events during germination, peaking earlier compared with the wild type. Promoter analysis of AtTim17-1 further identified an abscisic acid... (More)

In Arabidopsis (Arabidopsis thaliana), small gene families encode multiple isoforms for many of the components of the mitochondrial protein import apparatus. There are three isoforms of the TRANSLOCASE OF THE INNER MEMBRANE17 (Tim17). Transcriptome analysis indicates that AtTim17-1 is only detectable in dry seed. In this study, two independent transfer DNA insertional mutant lines of tim17-1 exhibited a germination-specific phenotype, showing a significant increase in the rate of germination. Microarray analyses revealed that Attim17-1 displayed alterations in the temporal sequence of transcriptomic events during germination, peaking earlier compared with the wild type. Promoter analysis of AtTim17-1 further identified an abscisic acid (ABA)-responsive element, which binds ABA-responsive transcription factors, acting to repress the expression of AtTim17-1. Attim17-1 dry seeds contained significantly increased levels of ABA and gibberellin, 2- and 5-fold, respectively. These results support the model that mitochondrial biogenesis is regulated in a tight temporal sequence of events during germination and that altering mitochondrial biogenesis feeds back to alter the germination rate, as evidenced by the altered levels of the master regulatory hormones that define germination.

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keywords
Abscisic Acid, Arabidopsis, Arabidopsis Proteins, Gene Expression Regulation, Plant, Gene Knockout Techniques, Germination, Gibberellins, Membrane Transport Proteins, Mitochondria, Mutation, Promoter Regions, Genetic, Protein Isoforms, Seeds, Time Factors, Journal Article, Research Support, Non-U.S. Gov't
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Plant Physiology
volume
166
issue
3
pages
16 pages
publisher
American Society of Plant Biologists
external identifiers
  • scopus:84908604667
ISSN
1532-2548
DOI
10.1104/pp.114.245928
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English
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f13e9e9c-b8fe-4664-8967-cca5e0352c52
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2017-05-08 10:48:01
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2017-09-10 05:18:38
@article{f13e9e9c-b8fe-4664-8967-cca5e0352c52,
  abstract     = {<p>In Arabidopsis (Arabidopsis thaliana), small gene families encode multiple isoforms for many of the components of the mitochondrial protein import apparatus. There are three isoforms of the TRANSLOCASE OF THE INNER MEMBRANE17 (Tim17). Transcriptome analysis indicates that AtTim17-1 is only detectable in dry seed. In this study, two independent transfer DNA insertional mutant lines of tim17-1 exhibited a germination-specific phenotype, showing a significant increase in the rate of germination. Microarray analyses revealed that Attim17-1 displayed alterations in the temporal sequence of transcriptomic events during germination, peaking earlier compared with the wild type. Promoter analysis of AtTim17-1 further identified an abscisic acid (ABA)-responsive element, which binds ABA-responsive transcription factors, acting to repress the expression of AtTim17-1. Attim17-1 dry seeds contained significantly increased levels of ABA and gibberellin, 2- and 5-fold, respectively. These results support the model that mitochondrial biogenesis is regulated in a tight temporal sequence of events during germination and that altering mitochondrial biogenesis feeds back to alter the germination rate, as evidenced by the altered levels of the master regulatory hormones that define germination.</p>},
  author       = {Wang, Yan and Law, Simon R and Ivanova, Aneta and van Aken, Olivier and Kubiszewski-Jakubiak, Szymon and Uggalla, Vindya and van der Merwe, Margaretha and Duncan, Owen and Narsai, Reena and Whelan, James and Murcha, Monika W},
  issn         = {1532-2548},
  keyword      = {Abscisic Acid,Arabidopsis,Arabidopsis Proteins,Gene Expression Regulation, Plant,Gene Knockout Techniques,Germination,Gibberellins,Membrane Transport Proteins,Mitochondria,Mutation,Promoter Regions, Genetic,Protein Isoforms,Seeds,Time Factors,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {35--1420},
  publisher    = {American Society of Plant Biologists},
  series       = {Plant Physiology},
  title        = {The mitochondrial protein import component, TRANSLOCASE OF THE INNER MEMBRANE17-1, plays a role in defining the timing of germination in Arabidopsis},
  url          = {http://dx.doi.org/10.1104/pp.114.245928},
  volume       = {166},
  year         = {2014},
}