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Levels of retinal IAPP are altered in Alzheimer's disease patients and correlate with vascular changes and hippocampal IAPP levels

Schultz, Nina LU ; Byman, Elin LU ; , and Wennström, Malin LU (2018) In Neurobiology of Aging 69. p.94-101
Abstract

Islet amyloid polypeptide (IAPP) forms toxic aggregates in the brain of patients with Alzheimer's disease (AD). Whether IAPP also affects the retina in these patients is still unknown. Levels of IAPP in soluble and insoluble homogenate fractions of retina and hippocampus from AD patients and nondemented controls were analyzed using ELISA. Number of pericytes and vessel length were determined by analysis of immunostained retina and hippocampus. Insoluble retinal fractions of AD patients contained lower levels of unmodified IAPP, whereas soluble retinal fractions contained increased levels of the same. Total IAPP levels and pericyte numbers in retina mirrored corresponding variables in the hippocampus. Moreover, levels of total unmodified... (More)

Islet amyloid polypeptide (IAPP) forms toxic aggregates in the brain of patients with Alzheimer's disease (AD). Whether IAPP also affects the retina in these patients is still unknown. Levels of IAPP in soluble and insoluble homogenate fractions of retina and hippocampus from AD patients and nondemented controls were analyzed using ELISA. Number of pericytes and vessel length were determined by analysis of immunostained retina and hippocampus. Insoluble retinal fractions of AD patients contained lower levels of unmodified IAPP, whereas soluble retinal fractions contained increased levels of the same. Total IAPP levels and pericyte numbers in retina mirrored corresponding variables in the hippocampus. Moreover, levels of total unmodified IAPP correlated negatively with the vessel length both in retina and hippocampus across the group and positively with pericyte numbers in retina in AD patients. Our studies indicate that changes in brain IAPP are reflected by corresponding levels in the retina. Our results also suggest modification of IAPP as an important event implicated in vascular changes associated with AD.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alzheimer's disease, Hippocampus, IAPP, Pericytes, Retina
in
Neurobiology of Aging
volume
69
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:85047876867
ISSN
0197-4580
DOI
10.1016/j.neurobiolaging.2018.05.003
language
English
LU publication?
yes
id
f180427b-8eeb-471c-a57c-c6bc3e9ce0ee
date added to LUP
2018-06-11 10:26:05
date last changed
2018-06-12 03:00:02
@article{f180427b-8eeb-471c-a57c-c6bc3e9ce0ee,
  abstract     = {<p>Islet amyloid polypeptide (IAPP) forms toxic aggregates in the brain of patients with Alzheimer's disease (AD). Whether IAPP also affects the retina in these patients is still unknown. Levels of IAPP in soluble and insoluble homogenate fractions of retina and hippocampus from AD patients and nondemented controls were analyzed using ELISA. Number of pericytes and vessel length were determined by analysis of immunostained retina and hippocampus. Insoluble retinal fractions of AD patients contained lower levels of unmodified IAPP, whereas soluble retinal fractions contained increased levels of the same. Total IAPP levels and pericyte numbers in retina mirrored corresponding variables in the hippocampus. Moreover, levels of total unmodified IAPP correlated negatively with the vessel length both in retina and hippocampus across the group and positively with pericyte numbers in retina in AD patients. Our studies indicate that changes in brain IAPP are reflected by corresponding levels in the retina. Our results also suggest modification of IAPP as an important event implicated in vascular changes associated with AD.</p>},
  author       = {Schultz, Nina and Byman, Elin and ,  and Wennström, Malin},
  issn         = {0197-4580},
  keyword      = {Alzheimer's disease,Hippocampus,IAPP,Pericytes,Retina},
  language     = {eng},
  month        = {09},
  pages        = {94--101},
  publisher    = {Elsevier},
  series       = {Neurobiology of Aging},
  title        = {Levels of retinal IAPP are altered in Alzheimer's disease patients and correlate with vascular changes and hippocampal IAPP levels},
  url          = {http://dx.doi.org/10.1016/j.neurobiolaging.2018.05.003},
  volume       = {69},
  year         = {2018},
}