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Lung Inflammatory Environments Differentially Alter Mesenchymal Stromal Cell Behavior

Abreu, Soraia Carvalho ; Enes, Sara Rolandsson LU ; Dearborn, Jacob ; Goodwin, Meagan ; Coffey, Amy ; Borg, Zachary D ; Dos Santos, Claudia C ; Wargo, Matthew J ; Cruz, Fernanda Ferreira and Loi, Roberto , et al. (2019) In American Journal of Physiology: Lung Cellular and Molecular Physiology 317(6). p.823-831
Abstract

Mesenchymal stromal (stem) cells (MSCs) are increasingly demonstrated to ameliorate experimentally-induced lung injuries through disease-specific anti-inflammatory actions, thus suggesting that different in vivo inflammatory environments can influence MSC actions. To determine the effects of different representative inflammatory lung conditions, human bone marrow-derived MSCs (hMSCs) were exposed to in vitro culture conditions from bronchoalveolar lavage fluid (BALF) samples obtained from patients with either the acute respiratory distress syndrome (ARDS) or with other lung diseases including acute respiratory exacerbations of cystic fibrosis (CF) (non-ARDS). hMSCs were subsequently assessed for time- and BALF concentration-dependent... (More)

Mesenchymal stromal (stem) cells (MSCs) are increasingly demonstrated to ameliorate experimentally-induced lung injuries through disease-specific anti-inflammatory actions, thus suggesting that different in vivo inflammatory environments can influence MSC actions. To determine the effects of different representative inflammatory lung conditions, human bone marrow-derived MSCs (hMSCs) were exposed to in vitro culture conditions from bronchoalveolar lavage fluid (BALF) samples obtained from patients with either the acute respiratory distress syndrome (ARDS) or with other lung diseases including acute respiratory exacerbations of cystic fibrosis (CF) (non-ARDS). hMSCs were subsequently assessed for time- and BALF concentration-dependent effects on mRNA expression of selected pro- and anti-inflammatory mediators, and for overall patterns of gene and mRNA expression. Both common and disease specific-patterns were observed in gene expression of different hMSC mediators, notably interleukin (IL)-6. Conditioned media obtained from non-ARDS BALF-exposed hMSCs was more effective in promoting an anti-inflammatory phenotype in monocytes than was conditioned media from ARDS BALF-exposed hMSCs. Neutralizing IL-6 in the conditioned media promoted generation of anti-inflammatory monocyte phenotype. These results demonstrated that different lung inflammatory environments differentially alter hMSC behavior. Further identification of these interactions and the driving mechanisms may influence clinical use of MSCs for treating lung diseases.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
American Journal of Physiology: Lung Cellular and Molecular Physiology
volume
317
issue
6
pages
9 pages
publisher
American Physiological Society
external identifiers
  • scopus:85076125829
  • pmid:31553626
ISSN
1522-1504
DOI
10.1152/ajplung.00263.2019
project
MSC polarisation towards a disease-specific anti-inflammatory phenotype in severe inflammatory lung disorders
language
English
LU publication?
yes
id
f181b6af-3b14-4451-b300-63f55ae0e252
date added to LUP
2019-12-02 12:46:54
date last changed
2021-01-26 01:59:58
@article{f181b6af-3b14-4451-b300-63f55ae0e252,
  abstract     = {<p>Mesenchymal stromal (stem) cells (MSCs) are increasingly demonstrated to ameliorate experimentally-induced lung injuries through disease-specific anti-inflammatory actions, thus suggesting that different in vivo inflammatory environments can influence MSC actions. To determine the effects of different representative inflammatory lung conditions, human bone marrow-derived MSCs (hMSCs) were exposed to in vitro culture conditions from bronchoalveolar lavage fluid (BALF) samples obtained from patients with either the acute respiratory distress syndrome (ARDS) or with other lung diseases including acute respiratory exacerbations of cystic fibrosis (CF) (non-ARDS). hMSCs were subsequently assessed for time- and BALF concentration-dependent effects on mRNA expression of selected pro- and anti-inflammatory mediators, and for overall patterns of gene and mRNA expression. Both common and disease specific-patterns were observed in gene expression of different hMSC mediators, notably interleukin (IL)-6. Conditioned media obtained from non-ARDS BALF-exposed hMSCs was more effective in promoting an anti-inflammatory phenotype in monocytes than was conditioned media from ARDS BALF-exposed hMSCs. Neutralizing IL-6 in the conditioned media promoted generation of anti-inflammatory monocyte phenotype. These results demonstrated that different lung inflammatory environments differentially alter hMSC behavior. Further identification of these interactions and the driving mechanisms may influence clinical use of MSCs for treating lung diseases.</p>},
  author       = {Abreu, Soraia Carvalho and Enes, Sara Rolandsson and Dearborn, Jacob and Goodwin, Meagan and Coffey, Amy and Borg, Zachary D and Dos Santos, Claudia C and Wargo, Matthew J and Cruz, Fernanda Ferreira and Loi, Roberto and DeSarno, Michael and Ashikaga, Takamaru and Antunes, Mariana Alves and Rocco, Patricia R M and Liu, Kathleen D and Lee, Jae-Woo and Matthay, Michael A and McKenna, D H and Weiss, Daniel J},
  issn         = {1522-1504},
  language     = {eng},
  month        = {12},
  number       = {6},
  pages        = {823--831},
  publisher    = {American Physiological Society},
  series       = {American Journal of Physiology: Lung Cellular and Molecular Physiology},
  title        = {Lung Inflammatory Environments Differentially Alter Mesenchymal Stromal Cell Behavior},
  url          = {http://dx.doi.org/10.1152/ajplung.00263.2019},
  doi          = {10.1152/ajplung.00263.2019},
  volume       = {317},
  year         = {2019},
}