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Novel tenascin variants with a distinctive pattern of expression in the avian embryo

Tucker, R P ; Spring, J ; Baumgartner, S LU orcid ; Martin, D ; Hagios, C ; Poss, P M and Chiquet-Ehrismann, R (1994) In Development (Cambridge) 120(3). p.47-637
Abstract

Previous studies have shown that several forms of the glycoprotein tenascin are present in the embryonic extracellular matrix. These forms are the result of alternative splicing, which generates tenascin variants with different numbers of fibronectin type III repeats. We have used degenerate primers and PCR to isolate a novel tenascin exon from an avian genomic library. Genomic clones contained a sequence encoding a fibronectin type III repeat that corresponds to repeat 'C' from the variable domain of human tenascin. To demonstrate that tenascin containing repeat 'C' is actually synthesized by avian cells, a monospecific antiserum was raised against a repeat 'C' fusion protein. This antiserum recognized a novel high-molecular-weight... (More)

Previous studies have shown that several forms of the glycoprotein tenascin are present in the embryonic extracellular matrix. These forms are the result of alternative splicing, which generates tenascin variants with different numbers of fibronectin type III repeats. We have used degenerate primers and PCR to isolate a novel tenascin exon from an avian genomic library. Genomic clones contained a sequence encoding a fibronectin type III repeat that corresponds to repeat 'C' from the variable domain of human tenascin. To demonstrate that tenascin containing repeat 'C' is actually synthesized by avian cells, a monospecific antiserum was raised against a repeat 'C' fusion protein. This antiserum recognized a novel high-molecular-weight variant on immunoblots of tenascin isolated from chicken embryo fibroblast-conditioned medium, and stained tendons on frozen sections of chicken embryos. A cDNA probe specific for mRNA encoding repeat 'C' was used for in situ hybridization. This probe hybridized in a subset of the embryonic tissues labelled with a universal tenascin probe, including tendons, ligaments and mesenchyme at sites of epithelial-mesenchymal interactions. Finally, we provide evidence that additional fibronectin type III repeats, one corresponding to a recently discovered human repeat as well as one entirely novel sequence, also exists in chicken tenascin mRNA. These data indicate that tenascin is present in the embryonic matrix in a multitude of forms and that these forms have distinctive distributions that may reflect more than one function for tenascin in development.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Amino Acid Sequence, Animals, Base Sequence, Cell Adhesion Molecules, Neuronal/genetics, Chick Embryo, Conserved Sequence, DNA/genetics, Extracellular Matrix/physiology, Extracellular Matrix Proteins/genetics, Humans, Immunohistochemistry, In Situ Hybridization, Models, Genetic, Molecular Sequence Data, Polymerase Chain Reaction, Repetitive Sequences, Nucleic Acid/genetics, Sequence Homology, Tenascin
in
Development (Cambridge)
volume
120
issue
3
pages
47 - 637
publisher
The Company of Biologists Ltd
external identifiers
  • pmid:7512896
  • scopus:0028206557
ISSN
0950-1991
language
English
LU publication?
no
id
f1aaecd7-8b4d-4fbd-b7df-0c2de8627acf
alternative location
https://dev.biologists.org/content/120/3/637.long
date added to LUP
2019-05-21 14:23:39
date last changed
2024-01-01 05:57:57
@article{f1aaecd7-8b4d-4fbd-b7df-0c2de8627acf,
  abstract     = {{<p>Previous studies have shown that several forms of the glycoprotein tenascin are present in the embryonic extracellular matrix. These forms are the result of alternative splicing, which generates tenascin variants with different numbers of fibronectin type III repeats. We have used degenerate primers and PCR to isolate a novel tenascin exon from an avian genomic library. Genomic clones contained a sequence encoding a fibronectin type III repeat that corresponds to repeat 'C' from the variable domain of human tenascin. To demonstrate that tenascin containing repeat 'C' is actually synthesized by avian cells, a monospecific antiserum was raised against a repeat 'C' fusion protein. This antiserum recognized a novel high-molecular-weight variant on immunoblots of tenascin isolated from chicken embryo fibroblast-conditioned medium, and stained tendons on frozen sections of chicken embryos. A cDNA probe specific for mRNA encoding repeat 'C' was used for in situ hybridization. This probe hybridized in a subset of the embryonic tissues labelled with a universal tenascin probe, including tendons, ligaments and mesenchyme at sites of epithelial-mesenchymal interactions. Finally, we provide evidence that additional fibronectin type III repeats, one corresponding to a recently discovered human repeat as well as one entirely novel sequence, also exists in chicken tenascin mRNA. These data indicate that tenascin is present in the embryonic matrix in a multitude of forms and that these forms have distinctive distributions that may reflect more than one function for tenascin in development.</p>}},
  author       = {{Tucker, R P and Spring, J and Baumgartner, S and Martin, D and Hagios, C and Poss, P M and Chiquet-Ehrismann, R}},
  issn         = {{0950-1991}},
  keywords     = {{Amino Acid Sequence; Animals; Base Sequence; Cell Adhesion Molecules, Neuronal/genetics; Chick Embryo; Conserved Sequence; DNA/genetics; Extracellular Matrix/physiology; Extracellular Matrix Proteins/genetics; Humans; Immunohistochemistry; In Situ Hybridization; Models, Genetic; Molecular Sequence Data; Polymerase Chain Reaction; Repetitive Sequences, Nucleic Acid/genetics; Sequence Homology; Tenascin}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{47--637}},
  publisher    = {{The Company of Biologists Ltd}},
  series       = {{Development (Cambridge)}},
  title        = {{Novel tenascin variants with a distinctive pattern of expression in the avian embryo}},
  url          = {{https://dev.biologists.org/content/120/3/637.long}},
  volume       = {{120}},
  year         = {{1994}},
}