Lactic acid fermentation in a recycle batch reactor using immobilized Lactobacillus casei.
(1997) In Biotechnology and Bioengineering 55(6). p.841-853- Abstract
- Lactic acid production by recycle batch fermentation using immobilized cells of Lactobacillus casei subsp. rhamnosus was studied. The culture medium was composed of whey treated with an endoprotease, and supplemented with 2.5 g/L of yeast extract and 0.18 mM Mn(2+) ions. The fermentation set-up comprised of a column packed with polyethyleneimine-coated foam glass particles, Pora-bact A, and connected with recirculation to a stirred tank reactor vessel for pH control. The immobilization of L. casei was performed simply by circulating the culture medium inoculated with the organism over the beads. At this stage, a long lag period preceded the cell growth and lactic acid production. Subsequently, for recycle batch fermentations using the... (More)
- Lactic acid production by recycle batch fermentation using immobilized cells of Lactobacillus casei subsp. rhamnosus was studied. The culture medium was composed of whey treated with an endoprotease, and supplemented with 2.5 g/L of yeast extract and 0.18 mM Mn(2+) ions. The fermentation set-up comprised of a column packed with polyethyleneimine-coated foam glass particles, Pora-bact A, and connected with recirculation to a stirred tank reactor vessel for pH control. The immobilization of L. casei was performed simply by circulating the culture medium inoculated with the organism over the beads. At this stage, a long lag period preceded the cell growth and lactic acid production. Subsequently, for recycle batch fermentations using the immobilized cells, the reducing sugar concentration of the medium was increased to 100 g/L by addition of glucose. The lactic acid production started immediately after onset of fermentation and the average reactor productivity during repeated cycles was about 4.3 to 4.6 g/L . h, with complete substrate utilization and more than 90% product yield. Sugar consumption and lactate yield were maintained at the same level with increase in medium volume up to at least 10 times that of the immobilized biocatalyst. The liberation of significant amounts of cells into the medium limited the number of fermentation cycles possible in a recycle batch mode. Use of lower yeast extract concentration reduced the amount of suspended biomass without significant change in productivity, thereby also increasing the number of fermentation cycles, and even maintained the D-lactate amount at low levels. The product was recovered from the clarified and decolorized broth by ion-exchange adsorption. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55:841-853, 1997. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1181039
- author
- Senthuran, A ; Senthuran, V ; Mattiasson, Bo LU and Kaul, Rajni LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- immobilized cells, lactic acid bacteria, adhesive fermentation, poly(ethyleneimine), recycle batch reactor
- in
- Biotechnology and Bioengineering
- volume
- 55
- issue
- 6
- pages
- 841 - 853
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:18636595
- scopus:0342378145
- ISSN
- 1097-0290
- DOI
- 10.1002/(SICI)1097-0290(19970920)55:6<841::AID-BIT3>3.0.CO;2-G
- language
- English
- LU publication?
- yes
- id
- f2179304-0395-469f-b26d-215da8b48952 (old id 1181039)
- date added to LUP
- 2016-04-01 12:20:57
- date last changed
- 2022-01-27 02:29:25
@article{f2179304-0395-469f-b26d-215da8b48952,
abstract = {{Lactic acid production by recycle batch fermentation using immobilized cells of Lactobacillus casei subsp. rhamnosus was studied. The culture medium was composed of whey treated with an endoprotease, and supplemented with 2.5 g/L of yeast extract and 0.18 mM Mn(2+) ions. The fermentation set-up comprised of a column packed with polyethyleneimine-coated foam glass particles, Pora-bact A, and connected with recirculation to a stirred tank reactor vessel for pH control. The immobilization of L. casei was performed simply by circulating the culture medium inoculated with the organism over the beads. At this stage, a long lag period preceded the cell growth and lactic acid production. Subsequently, for recycle batch fermentations using the immobilized cells, the reducing sugar concentration of the medium was increased to 100 g/L by addition of glucose. The lactic acid production started immediately after onset of fermentation and the average reactor productivity during repeated cycles was about 4.3 to 4.6 g/L . h, with complete substrate utilization and more than 90% product yield. Sugar consumption and lactate yield were maintained at the same level with increase in medium volume up to at least 10 times that of the immobilized biocatalyst. The liberation of significant amounts of cells into the medium limited the number of fermentation cycles possible in a recycle batch mode. Use of lower yeast extract concentration reduced the amount of suspended biomass without significant change in productivity, thereby also increasing the number of fermentation cycles, and even maintained the D-lactate amount at low levels. The product was recovered from the clarified and decolorized broth by ion-exchange adsorption. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55:841-853, 1997.}},
author = {{Senthuran, A and Senthuran, V and Mattiasson, Bo and Kaul, Rajni}},
issn = {{1097-0290}},
keywords = {{immobilized cells; lactic acid bacteria; adhesive fermentation; poly(ethyleneimine); recycle batch reactor}},
language = {{eng}},
number = {{6}},
pages = {{841--853}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{Lactic acid fermentation in a recycle batch reactor using immobilized Lactobacillus casei.}},
url = {{http://dx.doi.org/10.1002/(SICI)1097-0290(19970920)55:6<841::AID-BIT3>3.0.CO;2-G}},
doi = {{10.1002/(SICI)1097-0290(19970920)55:6<841::AID-BIT3>3.0.CO;2-G}},
volume = {{55}},
year = {{1997}},
}