Assessment of the TRX2p-yEGFP Biosensor to Monitor the Redox Response of an Industrial Xylose-Fermenting Saccharomyces cerevisiae Strain during Propagation and Fermentation
Perruca Foncillas, Raquel LU ; Sanchis Sebastiá, Miguel LU
; Wallberg, Ola
LU
; Carlquist, Magnus
LU
and Gorwa-Grauslund, Marie F.
LU
(2023)
In Journal of Fungi
9(6).
- Abstract
The commercial production of bioethanol from lignocellulosic biomass such as wheat straw requires utilizing a microorganism that can withstand all the stressors encountered in the process while fermenting all the sugars in the biomass. Therefore, it is essential to develop tools for monitoring and controlling the cellular fitness during both cell propagation and sugar fermentation to ethanol. In the present study, on-line flow cytometry was adopted to assess the response of the biosensor TRX2p-yEGFP for redox imbalance in an industrial xylose-fermenting strain of Saccharomyces cerevisiae during cell propagation and the following fermentation of wheat-straw hydrolysate. Rapid and transient induction of the sensor was recorded upon... (More)
The commercial production of bioethanol from lignocellulosic biomass such as wheat straw requires utilizing a microorganism that can withstand all the stressors encountered in the process while fermenting all the sugars in the biomass. Therefore, it is essential to develop tools for monitoring and controlling the cellular fitness during both cell propagation and sugar fermentation to ethanol. In the present study, on-line flow cytometry was adopted to assess the response of the biosensor TRX2p-yEGFP for redox imbalance in an industrial xylose-fermenting strain of Saccharomyces cerevisiae during cell propagation and the following fermentation of wheat-straw hydrolysate. Rapid and transient induction of the sensor was recorded upon exposure to furfural and wheat straw hydrolysate containing up to 3.8 g/L furfural. During the fermentation step, the induction rate of the sensor was also found to correlate to the initial ethanol production rate, highlighting the relevance of redox monitoring and the potential of the presented tool to assess the ethanol production rate in hydrolysates. Three different propagation strategies were also compared, and it was confirmed that pre-exposure to hydrolysate during propagation remains the most efficient method for high ethanol productivity in the following wheat-straw hydrolysate fermentations.
(Less)
- author
- Perruca Foncillas, Raquel
LU
; Sanchis Sebastiá, Miguel
LU
; Wallberg, Ola
LU
; Carlquist, Magnus
LU
and Gorwa-Grauslund, Marie F.
LU
- organization
- publishing date
- 2023-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- furfural, lignocellulosic bioethanol, on-line flow cytometry, redox imbalance, TRX2p-GFP, wheat-straw hydrolysate, yeast propagation
- in
- Journal of Fungi
- volume
- 9
- issue
- 6
- article number
- 630
- pages
- 17 pages
- publisher
- MDPI AG
- external identifiers
-
- pmid:37367566
- scopus:85163782880
- ISSN
- 2309-608X
- DOI
- 10.3390/jof9060630
- language
- English
- LU publication?
- yes
- id
- f22fafb6-404e-4e5d-892b-7741b70c5826
- date added to LUP
- 2023-08-20 11:48:42
- date last changed
- 2024-04-20 01:02:03
@article{f22fafb6-404e-4e5d-892b-7741b70c5826,
abstract = {{<p>The commercial production of bioethanol from lignocellulosic biomass such as wheat straw requires utilizing a microorganism that can withstand all the stressors encountered in the process while fermenting all the sugars in the biomass. Therefore, it is essential to develop tools for monitoring and controlling the cellular fitness during both cell propagation and sugar fermentation to ethanol. In the present study, on-line flow cytometry was adopted to assess the response of the biosensor TRX2p-yEGFP for redox imbalance in an industrial xylose-fermenting strain of Saccharomyces cerevisiae during cell propagation and the following fermentation of wheat-straw hydrolysate. Rapid and transient induction of the sensor was recorded upon exposure to furfural and wheat straw hydrolysate containing up to 3.8 g/L furfural. During the fermentation step, the induction rate of the sensor was also found to correlate to the initial ethanol production rate, highlighting the relevance of redox monitoring and the potential of the presented tool to assess the ethanol production rate in hydrolysates. Three different propagation strategies were also compared, and it was confirmed that pre-exposure to hydrolysate during propagation remains the most efficient method for high ethanol productivity in the following wheat-straw hydrolysate fermentations.</p>}},
author = {{Perruca Foncillas, Raquel and Sanchis Sebastiá, Miguel and Wallberg, Ola and Carlquist, Magnus and Gorwa-Grauslund, Marie F.}},
issn = {{2309-608X}},
keywords = {{furfural; lignocellulosic bioethanol; on-line flow cytometry; redox imbalance; TRX2p-GFP; wheat-straw hydrolysate; yeast propagation}},
language = {{eng}},
number = {{6}},
publisher = {{MDPI AG}},
series = {{Journal of Fungi}},
title = {{Assessment of the TRX2p-yEGFP Biosensor to Monitor the Redox Response of an Industrial Xylose-Fermenting Saccharomyces cerevisiae Strain during Propagation and Fermentation}},
url = {{http://dx.doi.org/10.3390/jof9060630}},
doi = {{10.3390/jof9060630}},
volume = {{9}},
year = {{2023}},
}