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Cellular aging dynamics after acute malaria infection : A 12-month longitudinal study

Asghar, Muhammad; Yman, Victor; Homann, Manijeh Vafa; Sondén, Klara; Hammar, Ulf; Hasselquist, Dennis LU and Färnert, Anna (2017) In Aging Cell
Abstract

Accelerated cellular aging and reduced lifespan have recently been shown in birds chronically infected with malaria parasites. Whether malaria infection also affects cellular aging in humans has not been reported. Here, we assessed the effect of a single acute Plasmodium falciparum malaria infection on cellular aging dynamics in travelers prospectively followed over one year in Sweden. DNA and RNA were extracted from venous blood collected at the time of admission and repeatedly up to one year. Telomere length was measured using real-time quantitative PCR, while telomerase activity and CDKN2A expression were measured by reverse transcriptase (RT)-qPCR. Our results show that acute malaria infection affects cellular aging as reflected by... (More)

Accelerated cellular aging and reduced lifespan have recently been shown in birds chronically infected with malaria parasites. Whether malaria infection also affects cellular aging in humans has not been reported. Here, we assessed the effect of a single acute Plasmodium falciparum malaria infection on cellular aging dynamics in travelers prospectively followed over one year in Sweden. DNA and RNA were extracted from venous blood collected at the time of admission and repeatedly up to one year. Telomere length was measured using real-time quantitative PCR, while telomerase activity and CDKN2A expression were measured by reverse transcriptase (RT)-qPCR. Our results show that acute malaria infection affects cellular aging as reflected by elevated levels of CDKN2A expression, lower telomerase activity, and substantial telomere shortening during the first three months postinfection. After that CDKN2A expression declined, telomerase activity increased and telomere length was gradually restored over one year, reflecting that cellular aging was reversed. These findings demonstrate that malaria infection affects cellular aging and the underlying cellular mechanism by which pathogens can affect host cellular aging and longevity need to be elucidated. Our results urge the need to investigate whether repeated malaria infections have more pronounced and long-lasting effects on cellular aging and lifespan (similarly to what was observed in birds) in populations living in malaria endemic areas.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
CDKN2A, Cellular aging, Malaria, Plasmodium falciparum, Telomerase, Telomeres
in
Aging Cell
publisher
Wiley-Blackwell
external identifiers
  • scopus:85034220529
ISSN
1474-9718
DOI
10.1111/acel.12702
language
English
LU publication?
yes
id
f25eae52-7daa-4b75-a139-547644de3b68
date added to LUP
2017-12-11 12:39:28
date last changed
2018-01-12 10:19:40
@article{f25eae52-7daa-4b75-a139-547644de3b68,
  abstract     = {<p>Accelerated cellular aging and reduced lifespan have recently been shown in birds chronically infected with malaria parasites. Whether malaria infection also affects cellular aging in humans has not been reported. Here, we assessed the effect of a single acute Plasmodium falciparum malaria infection on cellular aging dynamics in travelers prospectively followed over one year in Sweden. DNA and RNA were extracted from venous blood collected at the time of admission and repeatedly up to one year. Telomere length was measured using real-time quantitative PCR, while telomerase activity and CDKN2A expression were measured by reverse transcriptase (RT)-qPCR. Our results show that acute malaria infection affects cellular aging as reflected by elevated levels of CDKN2A expression, lower telomerase activity, and substantial telomere shortening during the first three months postinfection. After that CDKN2A expression declined, telomerase activity increased and telomere length was gradually restored over one year, reflecting that cellular aging was reversed. These findings demonstrate that malaria infection affects cellular aging and the underlying cellular mechanism by which pathogens can affect host cellular aging and longevity need to be elucidated. Our results urge the need to investigate whether repeated malaria infections have more pronounced and long-lasting effects on cellular aging and lifespan (similarly to what was observed in birds) in populations living in malaria endemic areas.</p>},
  author       = {Asghar, Muhammad and Yman, Victor and Homann, Manijeh Vafa and Sondén, Klara and Hammar, Ulf and Hasselquist, Dennis and Färnert, Anna},
  issn         = {1474-9718},
  keyword      = {CDKN2A,Cellular aging,Malaria,Plasmodium falciparum,Telomerase,Telomeres},
  language     = {eng},
  month        = {11},
  publisher    = {Wiley-Blackwell},
  series       = {Aging Cell},
  title        = {Cellular aging dynamics after acute malaria infection : A 12-month longitudinal study},
  url          = {http://dx.doi.org/10.1111/acel.12702},
  year         = {2017},
}