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Circulating Tumor Cell Kinetics and Morphology from the Liquid Biopsy Predict Disease Progression in Patients with Metastatic Colorectal Cancer Following Resection

Kolenčík, Drahomir ; Sachin, Narayan ; Thiele, Jana-Aletta ; McKinley, Dillon ; Sandström Gerdtsson, Anna LU ; Welter, Lisa ; Hošek, Petr ; Ostašov, Pavel ; Vyčítal, Ondrej and Brůha, Jan , et al. (2022) In Cancers 14(3).
Abstract
The liquid biopsy has the potential to improve current clinical practice in oncology by
providing real-time personalized information about a patient’s disease status and response to treatment. In this study, we evaluated 161 peripheral blood (PB) samples that were collected around
surgical resection from 47 metastatic colorectal cancer (mCRC) patients using the High-Definition
Single Cell Assay (HDSCA) workflow. In conjunction with the standard circulating tumor cell (CTC)
enumeration, cellular morphology and kinetics between time-points of collection were considered in
the survival analysis. CTCs, CTC-Apoptotic, and CTC clusters were found to indicate poor survival
with an increase in cell count from pre-resection... (More)
The liquid biopsy has the potential to improve current clinical practice in oncology by
providing real-time personalized information about a patient’s disease status and response to treatment. In this study, we evaluated 161 peripheral blood (PB) samples that were collected around
surgical resection from 47 metastatic colorectal cancer (mCRC) patients using the High-Definition
Single Cell Assay (HDSCA) workflow. In conjunction with the standard circulating tumor cell (CTC)
enumeration, cellular morphology and kinetics between time-points of collection were considered in
the survival analysis. CTCs, CTC-Apoptotic, and CTC clusters were found to indicate poor survival
with an increase in cell count from pre-resection to post-resection. This study demonstrates that
CTC subcategorization based on morphological differences leads to nuanced results between the
subtypes, emphasizing the heterogeneity within the CTC classification. Furthermore, we show that
factoring in the time-point of each blood collection is critical, both for its static enumeration and for
the change in cell populations between draws. By integrating morphology and time-based analysis
alongside standard CTC enumeration, liquid biopsy platforms can provide greater insight into the
pathophysiology of mCRC by highlighting the complexity of the disease across a patient’s treatment.
(Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cancers
volume
14
issue
3
publisher
MDPI AG
external identifiers
  • pmid:35158910
  • scopus:85123360950
ISSN
2072-6694
DOI
10.3390/cancers14030642
language
English
LU publication?
yes
id
f2b85e0b-c51d-4f7a-a855-551a373e5f63
date added to LUP
2022-03-25 21:43:12
date last changed
2023-05-28 04:00:38
@article{f2b85e0b-c51d-4f7a-a855-551a373e5f63,
  abstract     = {{The liquid biopsy has the potential to improve current clinical practice in oncology by<br/>providing real-time personalized information about a patient’s disease status and response to treatment. In this study, we evaluated 161 peripheral blood (PB) samples that were collected around<br/>surgical resection from 47 metastatic colorectal cancer (mCRC) patients using the High-Definition<br/>Single Cell Assay (HDSCA) workflow. In conjunction with the standard circulating tumor cell (CTC)<br/>enumeration, cellular morphology and kinetics between time-points of collection were considered in<br/>the survival analysis. CTCs, CTC-Apoptotic, and CTC clusters were found to indicate poor survival<br/>with an increase in cell count from pre-resection to post-resection. This study demonstrates that<br/>CTC subcategorization based on morphological differences leads to nuanced results between the<br/>subtypes, emphasizing the heterogeneity within the CTC classification. Furthermore, we show that<br/>factoring in the time-point of each blood collection is critical, both for its static enumeration and for<br/>the change in cell populations between draws. By integrating morphology and time-based analysis<br/>alongside standard CTC enumeration, liquid biopsy platforms can provide greater insight into the<br/>pathophysiology of mCRC by highlighting the complexity of the disease across a patient’s treatment.<br/>}},
  author       = {{Kolenčík, Drahomir and Sachin, Narayan and Thiele, Jana-Aletta and McKinley, Dillon and Sandström Gerdtsson, Anna and Welter, Lisa and Hošek, Petr and Ostašov, Pavel and Vyčítal, Ondrej and Brůha, Jan and Fiala, Ondrej and Šorejs, Ondrej and Liska, Vaclav and Pitule, Pavel and Kuhn, Peter and Shishido, Stephanie}},
  issn         = {{2072-6694}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{3}},
  publisher    = {{MDPI AG}},
  series       = {{Cancers}},
  title        = {{Circulating Tumor Cell Kinetics and Morphology from the Liquid Biopsy Predict Disease Progression in Patients with Metastatic Colorectal Cancer Following Resection}},
  url          = {{http://dx.doi.org/10.3390/cancers14030642}},
  doi          = {{10.3390/cancers14030642}},
  volume       = {{14}},
  year         = {{2022}},
}