Impact of double cryogelation process on a macroporous dye-affinity hydrogel
(2023) In Journal of Separation Science 46(8).- Abstract
Cryogels with interconnected channels allow high flow-through properties and mass transfer when dealing with complex mixtures such as non-clarified crude extracts. However, their mechanical strength can be challenged due to a large void volume inside the polymeric network. We have addressed this problem by forming a double-layer cryogel applied as a dye-affinity chromatography gel. In this study, poly(acrylamide-co-allyl glycidyl ether) cryogel was prepared at sub-zero temperature. The second layer was then prepared inside the primary cryogel under the same conditions to form a double-layer network. Cibacron Blue F3GA, a dye molecule, was immobilized on the surface of the cryogels. Bovine serum albumin was used as a model molecule to... (More)
Cryogels with interconnected channels allow high flow-through properties and mass transfer when dealing with complex mixtures such as non-clarified crude extracts. However, their mechanical strength can be challenged due to a large void volume inside the polymeric network. We have addressed this problem by forming a double-layer cryogel applied as a dye-affinity chromatography gel. In this study, poly(acrylamide-co-allyl glycidyl ether) cryogel was prepared at sub-zero temperature. The second layer was then prepared inside the primary cryogel under the same conditions to form a double-layer network. Cibacron Blue F3GA, a dye molecule, was immobilized on the surface of the cryogels. Bovine serum albumin was used as a model molecule to study the adsorption/elution procedure in batch and continuous modes. The maximum batch binding capacity and the dynamic binding capacity for the single-layer cryogel were 18 and 0.11, and for the double-layer cryogel were 7.5 and 0.9 mg/g of gel, respectively. However, the mechanical stability of the double-layer cryogel increased 7-fold (144 kPa). It was found that the kinetic and adsorption isotherms follow pseudo-second-order and Freundlich models, respectively. The regeneration of the columns after adsorption/elution cycles was evaluated, and no significant loss of capacity was observed after 10 cycles.
(Less)
- author
- Trinh, Thi Hoai Thu ; Ye, Lei LU and Hajizadeh, Solmaz LU
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- albumin, double-layer cryogel, dye-affinity chromatography, dynamic binding capacity, macroporous network
- in
- Journal of Separation Science
- volume
- 46
- issue
- 8
- article number
- 2300017
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:36780629
- scopus:85150796565
- ISSN
- 1615-9306
- DOI
- 10.1002/jssc.202300017
- language
- English
- LU publication?
- yes
- id
- f2cfde2c-c8c7-4402-9cc2-18666b5c65eb
- date added to LUP
- 2023-05-26 14:42:37
- date last changed
- 2024-09-07 11:32:35
@article{f2cfde2c-c8c7-4402-9cc2-18666b5c65eb, abstract = {{<p>Cryogels with interconnected channels allow high flow-through properties and mass transfer when dealing with complex mixtures such as non-clarified crude extracts. However, their mechanical strength can be challenged due to a large void volume inside the polymeric network. We have addressed this problem by forming a double-layer cryogel applied as a dye-affinity chromatography gel. In this study, poly(acrylamide-co-allyl glycidyl ether) cryogel was prepared at sub-zero temperature. The second layer was then prepared inside the primary cryogel under the same conditions to form a double-layer network. Cibacron Blue F3GA, a dye molecule, was immobilized on the surface of the cryogels. Bovine serum albumin was used as a model molecule to study the adsorption/elution procedure in batch and continuous modes. The maximum batch binding capacity and the dynamic binding capacity for the single-layer cryogel were 18 and 0.11, and for the double-layer cryogel were 7.5 and 0.9 mg/g of gel, respectively. However, the mechanical stability of the double-layer cryogel increased 7-fold (144 kPa). It was found that the kinetic and adsorption isotherms follow pseudo-second-order and Freundlich models, respectively. The regeneration of the columns after adsorption/elution cycles was evaluated, and no significant loss of capacity was observed after 10 cycles.</p>}}, author = {{Trinh, Thi Hoai Thu and Ye, Lei and Hajizadeh, Solmaz}}, issn = {{1615-9306}}, keywords = {{albumin; double-layer cryogel; dye-affinity chromatography; dynamic binding capacity; macroporous network}}, language = {{eng}}, number = {{8}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Separation Science}}, title = {{Impact of double cryogelation process on a macroporous dye-affinity hydrogel}}, url = {{http://dx.doi.org/10.1002/jssc.202300017}}, doi = {{10.1002/jssc.202300017}}, volume = {{46}}, year = {{2023}}, }