Impact of double cryogelation process on a macroporous dye-affinity hydrogel
Trinh, Thi Hoai Thu ; Ye, Lei LU
and Hajizadeh, Solmaz
LU
(2023)
In Journal of Separation Science
46(8).
- Abstract
Cryogels with interconnected channels allow high flow-through properties and mass transfer when dealing with complex mixtures such as non-clarified crude extracts. However, their mechanical strength can be challenged due to a large void volume inside the polymeric network. We have addressed this problem by forming a double-layer cryogel applied as a dye-affinity chromatography gel. In this study, poly(acrylamide-co-allyl glycidyl ether) cryogel was prepared at sub-zero temperature. The second layer was then prepared inside the primary cryogel under the same conditions to form a double-layer network. Cibacron Blue F3GA, a dye molecule, was immobilized on the surface of the cryogels. Bovine serum albumin was used as a model molecule to... (More)
Cryogels with interconnected channels allow high flow-through properties and mass transfer when dealing with complex mixtures such as non-clarified crude extracts. However, their mechanical strength can be challenged due to a large void volume inside the polymeric network. We have addressed this problem by forming a double-layer cryogel applied as a dye-affinity chromatography gel. In this study, poly(acrylamide-co-allyl glycidyl ether) cryogel was prepared at sub-zero temperature. The second layer was then prepared inside the primary cryogel under the same conditions to form a double-layer network. Cibacron Blue F3GA, a dye molecule, was immobilized on the surface of the cryogels. Bovine serum albumin was used as a model molecule to study the adsorption/elution procedure in batch and continuous modes. The maximum batch binding capacity and the dynamic binding capacity for the single-layer cryogel were 18 and 0.11, and for the double-layer cryogel were 7.5 and 0.9 mg/g of gel, respectively. However, the mechanical stability of the double-layer cryogel increased 7-fold (144 kPa). It was found that the kinetic and adsorption isotherms follow pseudo-second-order and Freundlich models, respectively. The regeneration of the columns after adsorption/elution cycles was evaluated, and no significant loss of capacity was observed after 10 cycles.
(Less)
- author
- Trinh, Thi Hoai Thu
; Ye, Lei
LU
and Hajizadeh, Solmaz
LU
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- albumin, double-layer cryogel, dye-affinity chromatography, dynamic binding capacity, macroporous network
- in
- Journal of Separation Science
- volume
- 46
- issue
- 8
- article number
- 2300017
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85150796565
- pmid:36780629
- ISSN
- 1615-9306
- DOI
- 10.1002/jssc.202300017
- language
- English
- LU publication?
- yes
- id
- f2cfde2c-c8c7-4402-9cc2-18666b5c65eb
- date added to LUP
- 2023-05-26 14:42:37
- date last changed
- 2024-06-15 03:28:09
@article{f2cfde2c-c8c7-4402-9cc2-18666b5c65eb,
abstract = {{<p>Cryogels with interconnected channels allow high flow-through properties and mass transfer when dealing with complex mixtures such as non-clarified crude extracts. However, their mechanical strength can be challenged due to a large void volume inside the polymeric network. We have addressed this problem by forming a double-layer cryogel applied as a dye-affinity chromatography gel. In this study, poly(acrylamide-co-allyl glycidyl ether) cryogel was prepared at sub-zero temperature. The second layer was then prepared inside the primary cryogel under the same conditions to form a double-layer network. Cibacron Blue F3GA, a dye molecule, was immobilized on the surface of the cryogels. Bovine serum albumin was used as a model molecule to study the adsorption/elution procedure in batch and continuous modes. The maximum batch binding capacity and the dynamic binding capacity for the single-layer cryogel were 18 and 0.11, and for the double-layer cryogel were 7.5 and 0.9 mg/g of gel, respectively. However, the mechanical stability of the double-layer cryogel increased 7-fold (144 kPa). It was found that the kinetic and adsorption isotherms follow pseudo-second-order and Freundlich models, respectively. The regeneration of the columns after adsorption/elution cycles was evaluated, and no significant loss of capacity was observed after 10 cycles.</p>}},
author = {{Trinh, Thi Hoai Thu and Ye, Lei and Hajizadeh, Solmaz}},
issn = {{1615-9306}},
keywords = {{albumin; double-layer cryogel; dye-affinity chromatography; dynamic binding capacity; macroporous network}},
language = {{eng}},
number = {{8}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Separation Science}},
title = {{Impact of double cryogelation process on a macroporous dye-affinity hydrogel}},
url = {{http://dx.doi.org/10.1002/jssc.202300017}},
doi = {{10.1002/jssc.202300017}},
volume = {{46}},
year = {{2023}},
}