Phenotypic map of porcine retinal ganglion cells
(2013) In Molecular Vision 19. p.16-904- Abstract
PURPOSE: Porcine retina is an excellent model for studying diverse retinal processes and diseases. The morphologies of porcine retinal ganglion cells (RGCs) have, however, not yet been described comprehensively. The aim of the present study was to créate a classification of the RGCs using the 1, 1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) tracing method.
METHODS: About 170 RGCs were retrogradely labeled by injecting DiI into the optic nerve of postmortem eyes and statistically analyzed by two different clustering methods: Ward's algorithm and the K-means clustering. Major axis length of the soma, soma area size, and dendritic field area size were selected as main parameters for cluster... (More)
PURPOSE: Porcine retina is an excellent model for studying diverse retinal processes and diseases. The morphologies of porcine retinal ganglion cells (RGCs) have, however, not yet been described comprehensively. The aim of the present study was to créate a classification of the RGCs using the 1, 1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) tracing method.
METHODS: About 170 RGCs were retrogradely labeled by injecting DiI into the optic nerve of postmortem eyes and statistically analyzed by two different clustering methods: Ward's algorithm and the K-means clustering. Major axis length of the soma, soma area size, and dendritic field area size were selected as main parameters for cluster classification.
RESULTS: RGC distribution in clusters was achieved according to their morphological parameters. It was feasible to combine both statistical methods, thereby obtaining a robust clustering distribution. Morphological analysis resulted in a classification of RGCs in three groups according to the soma size and dendritic field: A (large somas and large dendritic fields), B (medium to large somas and medium to large dendritic fields), C (medium to small somas and medium to small dendritic fields). Within groups, fine clustering defined several subgroups according to dendritic arborization and level of stratification. Additionally, cells stratifying in two different levels of the inner plexiform layer were observed within the clusters.
CONCLUSIONS: This comprehensive study of RGC morphologies in the porcine retina provides fundamental knowledge about RGC cell types and provides a basis for functional studies toward selective RGC cell degeneration in retinal disorders.
(Less)
- author
- Veiga-Crespo, Patricia LU ; del Río, Patricia ; Blindert, Marcel ; Ueffing, Marius ; Hauck, Stefanie M and Vecino, Elena
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Cell Count, Cell Size, Cluster Analysis, Dendrites, Models, Biological, Phenotype, Retinal Ganglion Cells, Sus scrofa, Journal Article, Research Support, Non-U.S. Gov't
- in
- Molecular Vision
- volume
- 19
- pages
- 13 pages
- publisher
- Molecular Vision
- external identifiers
-
- pmid:23687427
- scopus:84876572097
- ISSN
- 1090-0535
- language
- English
- LU publication?
- no
- id
- f2e231d7-d87f-4ae3-98a7-a57873bb347d
- date added to LUP
- 2016-12-13 14:52:35
- date last changed
- 2024-05-04 15:33:56
@article{f2e231d7-d87f-4ae3-98a7-a57873bb347d,
abstract = {{<p>PURPOSE: Porcine retina is an excellent model for studying diverse retinal processes and diseases. The morphologies of porcine retinal ganglion cells (RGCs) have, however, not yet been described comprehensively. The aim of the present study was to créate a classification of the RGCs using the 1, 1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) tracing method.</p><p>METHODS: About 170 RGCs were retrogradely labeled by injecting DiI into the optic nerve of postmortem eyes and statistically analyzed by two different clustering methods: Ward's algorithm and the K-means clustering. Major axis length of the soma, soma area size, and dendritic field area size were selected as main parameters for cluster classification.</p><p>RESULTS: RGC distribution in clusters was achieved according to their morphological parameters. It was feasible to combine both statistical methods, thereby obtaining a robust clustering distribution. Morphological analysis resulted in a classification of RGCs in three groups according to the soma size and dendritic field: A (large somas and large dendritic fields), B (medium to large somas and medium to large dendritic fields), C (medium to small somas and medium to small dendritic fields). Within groups, fine clustering defined several subgroups according to dendritic arborization and level of stratification. Additionally, cells stratifying in two different levels of the inner plexiform layer were observed within the clusters.</p><p>CONCLUSIONS: This comprehensive study of RGC morphologies in the porcine retina provides fundamental knowledge about RGC cell types and provides a basis for functional studies toward selective RGC cell degeneration in retinal disorders.</p>}},
author = {{Veiga-Crespo, Patricia and del Río, Patricia and Blindert, Marcel and Ueffing, Marius and Hauck, Stefanie M and Vecino, Elena}},
issn = {{1090-0535}},
keywords = {{Animals; Cell Count; Cell Size; Cluster Analysis; Dendrites; Models, Biological; Phenotype; Retinal Ganglion Cells; Sus scrofa; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
pages = {{16--904}},
publisher = {{Molecular Vision}},
series = {{Molecular Vision}},
title = {{Phenotypic map of porcine retinal ganglion cells}},
volume = {{19}},
year = {{2013}},
}