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Quantitative lymphoscintigraphy. I. Basic concepts for optimal uptake of radiocolloids in the parasternal lymph nodes of rabbits

Strand, S. E. LU and Persson, B. R.R. LU orcid (1979) In Journal of Nuclear Medicine 20(10). p.1038-1046
Abstract

The activity-size distribution of radiocolloids has been studied using gel-chromatography scanning (GCS) of columns filled with Sepharose 4B gel. Rabbits were injected subcutaneously with the colloid of interest, laid supine beneath a gamma camera, and imaged every 15 sec for 2 to 4 hr. From the stored data, the uptakes in the parasternal lymph nodes were analyzed in terms of a two-compartment model, and the rate constants measured. The substances tested were Au-198 colloid, Tc-99m antimony sulfide colloid, Tc-99m tin colloid, Tc-99m phytate, and Tc99m sulfur colloid. It was shown that the optimal particle size for the colloid is in the range 1-10 nm. The largest and most rapid uptake was found for Au-198 colloid, with a particle size... (More)

The activity-size distribution of radiocolloids has been studied using gel-chromatography scanning (GCS) of columns filled with Sepharose 4B gel. Rabbits were injected subcutaneously with the colloid of interest, laid supine beneath a gamma camera, and imaged every 15 sec for 2 to 4 hr. From the stored data, the uptakes in the parasternal lymph nodes were analyzed in terms of a two-compartment model, and the rate constants measured. The substances tested were Au-198 colloid, Tc-99m antimony sulfide colloid, Tc-99m tin colloid, Tc-99m phytate, and Tc99m sulfur colloid. It was shown that the optimal particle size for the colloid is in the range 1-10 nm. The largest and most rapid uptake was found for Au-198 colloid, with a particle size of 5 nm, which appeared as a single peak in the GCS spectrum. The percentage uptake after 2 hr for Au-198 colloid was 8%, while it was 5% for antimony sulfide colloid, which was the best of the Tc-99m-labeled colloids. The GCS spectrum for the antimony product showed a single-peaked size distribution with a somewhat broader range: 5-15 nm. The particles of the other colloids were either too large to pass into the lymphatic system, or too small to be trapped.

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published
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in
Journal of Nuclear Medicine
volume
20
issue
10
pages
1038 - 1046
publisher
Society of Nuclear Medicine
external identifiers
  • pmid:231640
  • scopus:0018688417
ISSN
0161-5505
language
English
LU publication?
yes
id
f2fb6784-9d7d-4a53-9199-d901e1422aed
alternative location
http://jnm.snmjournals.org/content/20/10/1038.full.pdf+html
date added to LUP
2020-08-15 17:29:11
date last changed
2024-04-03 12:03:08
@article{f2fb6784-9d7d-4a53-9199-d901e1422aed,
  abstract     = {{<p>The activity-size distribution of radiocolloids has been studied using gel-chromatography scanning (GCS) of columns filled with Sepharose 4B gel. Rabbits were injected subcutaneously with the colloid of interest, laid supine beneath a gamma camera, and imaged every 15 sec for 2 to 4 hr. From the stored data, the uptakes in the parasternal lymph nodes were analyzed in terms of a two-compartment model, and the rate constants measured. The substances tested were Au-198 colloid, Tc-99m antimony sulfide colloid, Tc-99m tin colloid, Tc-99m phytate, and Tc99m sulfur colloid. It was shown that the optimal particle size for the colloid is in the range 1-10 nm. The largest and most rapid uptake was found for Au-198 colloid, with a particle size of 5 nm, which appeared as a single peak in the GCS spectrum. The percentage uptake after 2 hr for Au-198 colloid was 8%, while it was 5% for antimony sulfide colloid, which was the best of the Tc-99m-labeled colloids. The GCS spectrum for the antimony product showed a single-peaked size distribution with a somewhat broader range: 5-15 nm. The particles of the other colloids were either too large to pass into the lymphatic system, or too small to be trapped.</p>}},
  author       = {{Strand, S. E. and Persson, B. R.R.}},
  issn         = {{0161-5505}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{10}},
  pages        = {{1038--1046}},
  publisher    = {{Society of Nuclear Medicine}},
  series       = {{Journal of Nuclear Medicine}},
  title        = {{Quantitative lymphoscintigraphy. I. Basic concepts for optimal uptake of radiocolloids in the parasternal lymph nodes of rabbits}},
  url          = {{http://jnm.snmjournals.org/content/20/10/1038.full.pdf+html}},
  volume       = {{20}},
  year         = {{1979}},
}