Quantitative lymphoscintigraphy. I. Basic concepts for optimal uptake of radiocolloids in the parasternal lymph nodes of rabbits
(1979) In Journal of Nuclear Medicine 20(10). p.1038-1046- Abstract
The activity-size distribution of radiocolloids has been studied using gel-chromatography scanning (GCS) of columns filled with Sepharose 4B gel. Rabbits were injected subcutaneously with the colloid of interest, laid supine beneath a gamma camera, and imaged every 15 sec for 2 to 4 hr. From the stored data, the uptakes in the parasternal lymph nodes were analyzed in terms of a two-compartment model, and the rate constants measured. The substances tested were Au-198 colloid, Tc-99m antimony sulfide colloid, Tc-99m tin colloid, Tc-99m phytate, and Tc99m sulfur colloid. It was shown that the optimal particle size for the colloid is in the range 1-10 nm. The largest and most rapid uptake was found for Au-198 colloid, with a particle size... (More)
The activity-size distribution of radiocolloids has been studied using gel-chromatography scanning (GCS) of columns filled with Sepharose 4B gel. Rabbits were injected subcutaneously with the colloid of interest, laid supine beneath a gamma camera, and imaged every 15 sec for 2 to 4 hr. From the stored data, the uptakes in the parasternal lymph nodes were analyzed in terms of a two-compartment model, and the rate constants measured. The substances tested were Au-198 colloid, Tc-99m antimony sulfide colloid, Tc-99m tin colloid, Tc-99m phytate, and Tc99m sulfur colloid. It was shown that the optimal particle size for the colloid is in the range 1-10 nm. The largest and most rapid uptake was found for Au-198 colloid, with a particle size of 5 nm, which appeared as a single peak in the GCS spectrum. The percentage uptake after 2 hr for Au-198 colloid was 8%, while it was 5% for antimony sulfide colloid, which was the best of the Tc-99m-labeled colloids. The GCS spectrum for the antimony product showed a single-peaked size distribution with a somewhat broader range: 5-15 nm. The particles of the other colloids were either too large to pass into the lymphatic system, or too small to be trapped.
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- author
- Strand, S. E. LU and Persson, B. R.R. LU
- organization
- publishing date
- 1979-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Nuclear Medicine
- volume
- 20
- issue
- 10
- pages
- 1038 - 1046
- publisher
- Society of Nuclear Medicine
- external identifiers
-
- pmid:231640
- scopus:0018688417
- ISSN
- 0161-5505
- language
- English
- LU publication?
- yes
- id
- f2fb6784-9d7d-4a53-9199-d901e1422aed
- alternative location
- http://jnm.snmjournals.org/content/20/10/1038.full.pdf+html
- date added to LUP
- 2020-08-15 17:29:11
- date last changed
- 2024-04-03 12:03:08
@article{f2fb6784-9d7d-4a53-9199-d901e1422aed, abstract = {{<p>The activity-size distribution of radiocolloids has been studied using gel-chromatography scanning (GCS) of columns filled with Sepharose 4B gel. Rabbits were injected subcutaneously with the colloid of interest, laid supine beneath a gamma camera, and imaged every 15 sec for 2 to 4 hr. From the stored data, the uptakes in the parasternal lymph nodes were analyzed in terms of a two-compartment model, and the rate constants measured. The substances tested were Au-198 colloid, Tc-99m antimony sulfide colloid, Tc-99m tin colloid, Tc-99m phytate, and Tc99m sulfur colloid. It was shown that the optimal particle size for the colloid is in the range 1-10 nm. The largest and most rapid uptake was found for Au-198 colloid, with a particle size of 5 nm, which appeared as a single peak in the GCS spectrum. The percentage uptake after 2 hr for Au-198 colloid was 8%, while it was 5% for antimony sulfide colloid, which was the best of the Tc-99m-labeled colloids. The GCS spectrum for the antimony product showed a single-peaked size distribution with a somewhat broader range: 5-15 nm. The particles of the other colloids were either too large to pass into the lymphatic system, or too small to be trapped.</p>}}, author = {{Strand, S. E. and Persson, B. R.R.}}, issn = {{0161-5505}}, language = {{eng}}, month = {{12}}, number = {{10}}, pages = {{1038--1046}}, publisher = {{Society of Nuclear Medicine}}, series = {{Journal of Nuclear Medicine}}, title = {{Quantitative lymphoscintigraphy. I. Basic concepts for optimal uptake of radiocolloids in the parasternal lymph nodes of rabbits}}, url = {{http://jnm.snmjournals.org/content/20/10/1038.full.pdf+html}}, volume = {{20}}, year = {{1979}}, }