Antiviral compounds obtained from microalgae commonly used as carotenoid sources
(2012) In Journal of Applied Phycology 24(4). p.731-741- Abstract
Pressurized liquid extraction (PLE), an environmentally friendly technique, has been used to obtain antiviral compounds from microalgae commonly used as carotenoid sources: Haematococcus pluvialis and Dunaliella salina. The antiviral properties of PLE extracts (hexane, ethanol and water) were evaluated against herpes simplex virus type 1 (HSV-1) at different stages during viral infection. Pretreatment of Vero cells with 75 μg mL -1 of H. pluvialis ethanol extract inhibited virus infection by approximately 85%, whereas the same concentration of water and hexane extracts reduced the virus infectivity 75% and 50%, respectively. D. salina extracts were less effective than H. pluvialis extracts and presented a different behaviour... (More)
Pressurized liquid extraction (PLE), an environmentally friendly technique, has been used to obtain antiviral compounds from microalgae commonly used as carotenoid sources: Haematococcus pluvialis and Dunaliella salina. The antiviral properties of PLE extracts (hexane, ethanol and water) were evaluated against herpes simplex virus type 1 (HSV-1) at different stages during viral infection. Pretreatment of Vero cells with 75 μg mL -1 of H. pluvialis ethanol extract inhibited virus infection by approximately 85%, whereas the same concentration of water and hexane extracts reduced the virus infectivity 75% and 50%, respectively. D. salina extracts were less effective than H. pluvialis extracts and presented a different behaviour since water and ethanol extracts produced a similar virus inhibition (65%). Moreover, H. pluvialis ethanol extract was also the most effective against HSV-1 intracellular replication. The antiviral activity of water PLE extracts was found to correlate with polysaccharides since the polysaccharide-rich fraction isolated from these extracts showed higher antiviral activity than the original water extracts. A gas chromatography-mass spectrometry (GC-MS) characterization of the H. pluvialis ethanol extract showed the antiviral activity of this extract could be partially related with the presence of short-chain fatty acids, although other compounds could be involved in this activity; meanwhile, in the case of D. salina ethanol extract other compounds seemed to be implied, such as: β-ionone, neophytadiene, phytol, palmitic acid and α-linolenic acid. The results demonstrate the use of PLE allows obtaining antiviral compounds from microalgae used as carotenoids sources, which gives the microalgae biomass an added value.
(Less)
- author
- Santoyo, Susana ; Jaime, Laura ; Plaza, Merichel LU ; Herrero, Miguel ; Rodriguez-Meizoso, Irene LU ; Ibañez, Elena and Reglero, Guillermo
- publishing date
- 2012-08
- type
- Contribution to journal
- publication status
- published
- keywords
- Antiviral activity, Dunaliella salina, Haematococcus pluvialis, Microalgae, Pressurized liquid extraction
- in
- Journal of Applied Phycology
- volume
- 24
- issue
- 4
- pages
- 11 pages
- publisher
- Springer
- external identifiers
-
- scopus:84864120513
- ISSN
- 0921-8971
- DOI
- 10.1007/s10811-011-9692-1
- language
- English
- LU publication?
- no
- id
- f32c1390-1d87-4854-a3f7-ad44e024b7e4
- date added to LUP
- 2017-04-10 10:22:52
- date last changed
- 2022-03-01 21:01:25
@article{f32c1390-1d87-4854-a3f7-ad44e024b7e4,
abstract = {{<p>Pressurized liquid extraction (PLE), an environmentally friendly technique, has been used to obtain antiviral compounds from microalgae commonly used as carotenoid sources: Haematococcus pluvialis and Dunaliella salina. The antiviral properties of PLE extracts (hexane, ethanol and water) were evaluated against herpes simplex virus type 1 (HSV-1) at different stages during viral infection. Pretreatment of Vero cells with 75 μg mL <sup>-1</sup> of H. pluvialis ethanol extract inhibited virus infection by approximately 85%, whereas the same concentration of water and hexane extracts reduced the virus infectivity 75% and 50%, respectively. D. salina extracts were less effective than H. pluvialis extracts and presented a different behaviour since water and ethanol extracts produced a similar virus inhibition (65%). Moreover, H. pluvialis ethanol extract was also the most effective against HSV-1 intracellular replication. The antiviral activity of water PLE extracts was found to correlate with polysaccharides since the polysaccharide-rich fraction isolated from these extracts showed higher antiviral activity than the original water extracts. A gas chromatography-mass spectrometry (GC-MS) characterization of the H. pluvialis ethanol extract showed the antiviral activity of this extract could be partially related with the presence of short-chain fatty acids, although other compounds could be involved in this activity; meanwhile, in the case of D. salina ethanol extract other compounds seemed to be implied, such as: β-ionone, neophytadiene, phytol, palmitic acid and α-linolenic acid. The results demonstrate the use of PLE allows obtaining antiviral compounds from microalgae used as carotenoids sources, which gives the microalgae biomass an added value.</p>}},
author = {{Santoyo, Susana and Jaime, Laura and Plaza, Merichel and Herrero, Miguel and Rodriguez-Meizoso, Irene and Ibañez, Elena and Reglero, Guillermo}},
issn = {{0921-8971}},
keywords = {{Antiviral activity; Dunaliella salina; Haematococcus pluvialis; Microalgae; Pressurized liquid extraction}},
language = {{eng}},
number = {{4}},
pages = {{731--741}},
publisher = {{Springer}},
series = {{Journal of Applied Phycology}},
title = {{Antiviral compounds obtained from microalgae commonly used as carotenoid sources}},
url = {{http://dx.doi.org/10.1007/s10811-011-9692-1}},
doi = {{10.1007/s10811-011-9692-1}},
volume = {{24}},
year = {{2012}},
}