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Expression and Purification of Intrinsically Disordered Aβ Peptide and Setup of Reproducible Aggregation Kinetics Experiment

Linse, Sara LU (2020) In Methods in molecular biology (Clifton, N.J.) 2141. p.731-754
Abstract

High purity and sequence homogeneity of intrinsically disordered proteins are prerequisites for reproducible studies of the kinetics and equilibrium of their self-assembly reactions. Starting from the pure state enables quantitative studies of intrinsic and extrinsic factors in the process to understand its molecular determinants. Here we outline detailed protocols for recombinant expression and purification of ultra-pure amyloid β peptide (Aβ) in sequence homogeneous form, which allows for the setup of reproducible kinetic self-assembly experiments.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aggregation mechanism, Alzheimer’s disease, Intrinsically disordered protein, Protein expression and purification, Reproducible kinetics experiment, Self-assembly process
in
Methods in molecular biology (Clifton, N.J.)
volume
2141
pages
24 pages
publisher
Springer
external identifiers
  • scopus:85088516309
  • pmid:32696387
ISSN
1940-6029
DOI
10.1007/978-1-0716-0524-0_38
language
English
LU publication?
yes
id
f35c90de-f302-4aa1-8a02-feaa56dac029
date added to LUP
2020-08-05 11:26:56
date last changed
2024-03-20 13:45:13
@article{f35c90de-f302-4aa1-8a02-feaa56dac029,
  abstract     = {{<p>High purity and sequence homogeneity of intrinsically disordered proteins are prerequisites for reproducible studies of the kinetics and equilibrium of their self-assembly reactions. Starting from the pure state enables quantitative studies of intrinsic and extrinsic factors in the process to understand its molecular determinants. Here we outline detailed protocols for recombinant expression and purification of ultra-pure amyloid β peptide (Aβ) in sequence homogeneous form, which allows for the setup of reproducible kinetic self-assembly experiments.</p>}},
  author       = {{Linse, Sara}},
  issn         = {{1940-6029}},
  keywords     = {{Aggregation mechanism; Alzheimer’s disease; Intrinsically disordered protein; Protein expression and purification; Reproducible kinetics experiment; Self-assembly process}},
  language     = {{eng}},
  pages        = {{731--754}},
  publisher    = {{Springer}},
  series       = {{Methods in molecular biology (Clifton, N.J.)}},
  title        = {{Expression and Purification of Intrinsically Disordered Aβ Peptide and Setup of Reproducible Aggregation Kinetics Experiment}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-0524-0_38}},
  doi          = {{10.1007/978-1-0716-0524-0_38}},
  volume       = {{2141}},
  year         = {{2020}},
}