Expression and Purification of Intrinsically Disordered Aβ Peptide and Setup of Reproducible Aggregation Kinetics Experiment
(2020) In Methods in molecular biology (Clifton, N.J.) 2141. p.731-754- Abstract
High purity and sequence homogeneity of intrinsically disordered proteins are prerequisites for reproducible studies of the kinetics and equilibrium of their self-assembly reactions. Starting from the pure state enables quantitative studies of intrinsic and extrinsic factors in the process to understand its molecular determinants. Here we outline detailed protocols for recombinant expression and purification of ultra-pure amyloid β peptide (Aβ) in sequence homogeneous form, which allows for the setup of reproducible kinetic self-assembly experiments.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/f35c90de-f302-4aa1-8a02-feaa56dac029
- author
- Linse, Sara LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aggregation mechanism, Alzheimer’s disease, Intrinsically disordered protein, Protein expression and purification, Reproducible kinetics experiment, Self-assembly process
- in
- Methods in molecular biology (Clifton, N.J.)
- volume
- 2141
- pages
- 24 pages
- publisher
- Springer
- external identifiers
-
- scopus:85088516309
- pmid:32696387
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-0716-0524-0_38
- language
- English
- LU publication?
- yes
- id
- f35c90de-f302-4aa1-8a02-feaa56dac029
- date added to LUP
- 2020-08-05 11:26:56
- date last changed
- 2024-03-20 13:45:13
@article{f35c90de-f302-4aa1-8a02-feaa56dac029, abstract = {{<p>High purity and sequence homogeneity of intrinsically disordered proteins are prerequisites for reproducible studies of the kinetics and equilibrium of their self-assembly reactions. Starting from the pure state enables quantitative studies of intrinsic and extrinsic factors in the process to understand its molecular determinants. Here we outline detailed protocols for recombinant expression and purification of ultra-pure amyloid β peptide (Aβ) in sequence homogeneous form, which allows for the setup of reproducible kinetic self-assembly experiments.</p>}}, author = {{Linse, Sara}}, issn = {{1940-6029}}, keywords = {{Aggregation mechanism; Alzheimer’s disease; Intrinsically disordered protein; Protein expression and purification; Reproducible kinetics experiment; Self-assembly process}}, language = {{eng}}, pages = {{731--754}}, publisher = {{Springer}}, series = {{Methods in molecular biology (Clifton, N.J.)}}, title = {{Expression and Purification of Intrinsically Disordered Aβ Peptide and Setup of Reproducible Aggregation Kinetics Experiment}}, url = {{http://dx.doi.org/10.1007/978-1-0716-0524-0_38}}, doi = {{10.1007/978-1-0716-0524-0_38}}, volume = {{2141}}, year = {{2020}}, }