Isolation and characterization of two minor fractions of α1,-antitrypsin by high-performance liquid chromatographic chromatofocusing
(1985) In Journal of Chromatography A 327. p.173-177- Abstract
α1-Antitrypsin is a glycoprotein that separates into five electrophoretic fractions, viz. M2, M4, M6, M7 and M8. Con A-Sepharose separates the protein into three fractions according to the branching degree of the three oligosaccharide chains. The Con A affinity is identical for M4 and M7 and for M6 and M8. Within each pair the proteins were isolated by rapid chromatofocusing. The M7 and M8 have the same carbohydrate structure as the major M4 and M6 respectively, but have lost the first five N-terminal amino acids (Glu-Asp-Pro-Glu-Gly) as compared to the majority of the protein.
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- author
- Jeppsson, Jan olof LU ; Lilja, Hans LU and Johansson, Maria
- organization
- publishing date
- 1985-06-26
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Chromatography A
- volume
- 327
- pages
- 173 - 177
- publisher
- Elsevier
- external identifiers
-
- scopus:0021890954
- pmid:3875624
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(01)81646-0
- language
- English
- LU publication?
- yes
- id
- f3a53862-d164-43d0-ad5a-99a46595e1e2
- date added to LUP
- 2022-12-06 16:59:52
- date last changed
- 2024-01-03 19:32:27
@article{f3a53862-d164-43d0-ad5a-99a46595e1e2, abstract = {{<p>α<sub>1</sub>-Antitrypsin is a glycoprotein that separates into five electrophoretic fractions, viz. M<sub>2</sub>, M<sub>4</sub>, M<sub>6</sub>, M<sub>7</sub> and M<sub>8</sub>. Con A-Sepharose separates the protein into three fractions according to the branching degree of the three oligosaccharide chains. The Con A affinity is identical for M<sub>4</sub> and M<sub>7</sub> and for M<sub>6</sub> and M<sub>8</sub>. Within each pair the proteins were isolated by rapid chromatofocusing. The M<sub>7</sub> and M<sub>8</sub> have the same carbohydrate structure as the major M<sub>4</sub> and M<sub>6</sub> respectively, but have lost the first five N-terminal amino acids (Glu-Asp-Pro-Glu-Gly) as compared to the majority of the protein.</p>}}, author = {{Jeppsson, Jan olof and Lilja, Hans and Johansson, Maria}}, issn = {{0021-9673}}, language = {{eng}}, month = {{06}}, pages = {{173--177}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography A}}, title = {{Isolation and characterization of two minor fractions of α<sub>1</sub>,-antitrypsin by high-performance liquid chromatographic chromatofocusing}}, url = {{http://dx.doi.org/10.1016/S0021-9673(01)81646-0}}, doi = {{10.1016/S0021-9673(01)81646-0}}, volume = {{327}}, year = {{1985}}, }