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Isolation and characterization of two minor fractions of α1,-antitrypsin by high-performance liquid chromatographic chromatofocusing

Jeppsson, Jan olof LU ; Lilja, Hans LU orcid and Johansson, Maria (1985) In Journal of Chromatography A 327. p.173-177
Abstract

α1-Antitrypsin is a glycoprotein that separates into five electrophoretic fractions, viz. M2, M4, M6, M7 and M8. Con A-Sepharose separates the protein into three fractions according to the branching degree of the three oligosaccharide chains. The Con A affinity is identical for M4 and M7 and for M6 and M8. Within each pair the proteins were isolated by rapid chromatofocusing. The M7 and M8 have the same carbohydrate structure as the major M4 and M6 respectively, but have lost the first five N-terminal amino acids (Glu-Asp-Pro-Glu-Gly) as compared to the majority of the protein.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Chromatography A
volume
327
pages
173 - 177
publisher
Elsevier
external identifiers
  • scopus:0021890954
  • pmid:3875624
ISSN
0021-9673
DOI
10.1016/S0021-9673(01)81646-0
language
English
LU publication?
yes
id
f3a53862-d164-43d0-ad5a-99a46595e1e2
date added to LUP
2022-12-06 16:59:52
date last changed
2024-01-03 19:32:27
@article{f3a53862-d164-43d0-ad5a-99a46595e1e2,
  abstract     = {{<p>α<sub>1</sub>-Antitrypsin is a glycoprotein that separates into five electrophoretic fractions, viz. M<sub>2</sub>, M<sub>4</sub>, M<sub>6</sub>, M<sub>7</sub> and M<sub>8</sub>. Con A-Sepharose separates the protein into three fractions according to the branching degree of the three oligosaccharide chains. The Con A affinity is identical for M<sub>4</sub> and M<sub>7</sub> and for M<sub>6</sub> and M<sub>8</sub>. Within each pair the proteins were isolated by rapid chromatofocusing. The M<sub>7</sub> and M<sub>8</sub> have the same carbohydrate structure as the major M<sub>4</sub> and M<sub>6</sub> respectively, but have lost the first five N-terminal amino acids (Glu-Asp-Pro-Glu-Gly) as compared to the majority of the protein.</p>}},
  author       = {{Jeppsson, Jan olof and Lilja, Hans and Johansson, Maria}},
  issn         = {{0021-9673}},
  language     = {{eng}},
  month        = {{06}},
  pages        = {{173--177}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography A}},
  title        = {{Isolation and characterization of two minor fractions of α<sub>1</sub>,-antitrypsin by high-performance liquid chromatographic chromatofocusing}},
  url          = {{http://dx.doi.org/10.1016/S0021-9673(01)81646-0}},
  doi          = {{10.1016/S0021-9673(01)81646-0}},
  volume       = {{327}},
  year         = {{1985}},
}