Antibody array generation and use.
(2014) In Methods in Molecular Biology 1131. p.563-571- Abstract
- Affinity proteomics, represented by antibody arrays, is a multiplex technology for high-throughput protein expression profiling of crude proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are individually deposited in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified using mainly fluorescence as the mode of detection. The binding pattern is then converted into a relative protein expression map, or protein atlas, delineating the composition of the sample at the molecular level. The technology provides unique opportunities for various applications, such as protein expression profiling, biomarker discovery, disease... (More)
- Affinity proteomics, represented by antibody arrays, is a multiplex technology for high-throughput protein expression profiling of crude proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are individually deposited in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified using mainly fluorescence as the mode of detection. The binding pattern is then converted into a relative protein expression map, or protein atlas, delineating the composition of the sample at the molecular level. The technology provides unique opportunities for various applications, such as protein expression profiling, biomarker discovery, disease diagnostics, prognostics, evidence-based therapy selection, and disease monitoring. Here, we describe the generation and use of planar antibody arrays for serum protein profiling. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4334933
- author
- Borrebaeck, Carl LU and Wingren, Christer LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods in Molecular Biology
- volume
- 1131
- pages
- 563 - 571
- publisher
- Springer
- external identifiers
-
- pmid:24515491
- scopus:84934438179
- pmid:24515491
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-62703-992-5_36
- language
- English
- LU publication?
- yes
- id
- f411ee2e-abaf-4a16-b8af-d94116a4ebb9 (old id 4334933)
- date added to LUP
- 2016-04-01 09:57:31
- date last changed
- 2022-03-12 00:47:24
@article{f411ee2e-abaf-4a16-b8af-d94116a4ebb9,
abstract = {{Affinity proteomics, represented by antibody arrays, is a multiplex technology for high-throughput protein expression profiling of crude proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are individually deposited in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified using mainly fluorescence as the mode of detection. The binding pattern is then converted into a relative protein expression map, or protein atlas, delineating the composition of the sample at the molecular level. The technology provides unique opportunities for various applications, such as protein expression profiling, biomarker discovery, disease diagnostics, prognostics, evidence-based therapy selection, and disease monitoring. Here, we describe the generation and use of planar antibody arrays for serum protein profiling.}},
author = {{Borrebaeck, Carl and Wingren, Christer}},
issn = {{1940-6029}},
language = {{eng}},
pages = {{563--571}},
publisher = {{Springer}},
series = {{Methods in Molecular Biology}},
title = {{Antibody array generation and use.}},
url = {{http://dx.doi.org/10.1007/978-1-62703-992-5_36}},
doi = {{10.1007/978-1-62703-992-5_36}},
volume = {{1131}},
year = {{2014}},
}