Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus
(1986) In Antiviral Research 6(2). p.103-112- Abstract
- A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis... (More)
- A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1103497
- author
- Widell, Anders LU ; Hansson, Bengt-Göran LU ; Öberg, Bo and Nordenfelt, Erik
- organization
- publishing date
- 1986
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ribavirin, amantadine, arabinosylcytosine, hepatitis A
- in
- Antiviral Research
- volume
- 6
- issue
- 2
- pages
- 103 - 112
- publisher
- Elsevier
- external identifiers
-
- pmid:3010855
- scopus:0022586905
- ISSN
- 0166-3542
- DOI
- 10.1016/0166-3542(86)90030-6
- language
- English
- LU publication?
- yes
- id
- f43ee2d9-f679-4a0e-b6f2-9a64a9da01a2 (old id 1103497)
- date added to LUP
- 2016-04-01 11:59:57
- date last changed
- 2021-10-03 04:33:19
@article{f43ee2d9-f679-4a0e-b6f2-9a64a9da01a2, abstract = {{A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV.}}, author = {{Widell, Anders and Hansson, Bengt-Göran and Öberg, Bo and Nordenfelt, Erik}}, issn = {{0166-3542}}, keywords = {{ribavirin; amantadine; arabinosylcytosine; hepatitis A}}, language = {{eng}}, number = {{2}}, pages = {{103--112}}, publisher = {{Elsevier}}, series = {{Antiviral Research}}, title = {{Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus}}, url = {{http://dx.doi.org/10.1016/0166-3542(86)90030-6}}, doi = {{10.1016/0166-3542(86)90030-6}}, volume = {{6}}, year = {{1986}}, }