Optical responses, permeability and diol-specific reactivity of thin polyacrylamide gels containing immobilized phenylboronic acid
(2008) In Polymer 49(6). p.1444-1454- Abstract
- Thin semitransparent gels were prepared by radical copolymerization of N-acryloyl-m-aminophenylboronic acid (NAAPBA) and acrylamide (AAm) taken in molar ratios from 8:92 to 16:84, respectively, in water. The gels were characterized by the content of immobilized NAAPBA and monomer conversion. Scanning electron microscopy revealed the micrometer size pores in the dried gels. The wet gels displayed a linear optical response to sugars with sensitivity decreasing in the series: D-fructose, D-galactose, D-glucose, D-mannose, N-acetyl-D-glucosamine in the sugar concentration range from 1 to 40-60 mM at pH 7.3. Cross-linking of the gels with N,N-methylene-bis-acrylamide decreased the strength of optical response. Specific binding capacity of a... (More)
- Thin semitransparent gels were prepared by radical copolymerization of N-acryloyl-m-aminophenylboronic acid (NAAPBA) and acrylamide (AAm) taken in molar ratios from 8:92 to 16:84, respectively, in water. The gels were characterized by the content of immobilized NAAPBA and monomer conversion. Scanning electron microscopy revealed the micrometer size pores in the dried gels. The wet gels displayed a linear optical response to sugars with sensitivity decreasing in the series: D-fructose, D-galactose, D-glucose, D-mannose, N-acetyl-D-glucosamine in the sugar concentration range from 1 to 40-60 mM at pH 7.3. Cross-linking of the gels with N,N-methylene-bis-acrylamide decreased the strength of optical response. Specific binding capacity of a diol-containing dye Alizarin Red S in the gels at pH 7.0 coincided with the content of immobilized NAAPBA indicating the 1: 1 stoichiometry of the reaction and, therefore, good accessibility of the boronic acid ligands for water-soluble diols. Permeability of the gels was studied with a non-interacting dye Ethyl Orange exhibiting the pore diffusion coefficient of 1.4 x 10(-7) cm(2)/s. The rate of optical response of the gels to glucose was found to be determined by diffusion of sugar into the relatively thick gels (1 = 0.35-1 mm) with effective diffusion coefficients of 2 x 10(-7) cm(2)/S. In the thinner gels (l = 0.1 mm) the input of other kinetic processes, such as affinity binding or structural rearrangements of the gel, was noticeable. (c) 2008 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1206596
- author
- Kuzimenkova, Marina LU ; Ivanov, Alexander LU ; Thammakhet, Chongdee ; Mikhalovska, Lyubov I. ; Galaev, Igor LU ; Thavarungkul, Panote ; Kanatharana, Proespichaya and Mattiasson, Bo LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- boronic acid, hydrogels, sugar sensing
- in
- Polymer
- volume
- 49
- issue
- 6
- pages
- 1444 - 1454
- publisher
- Elsevier
- external identifiers
-
- wos:000254818900004
- scopus:40249091260
- ISSN
- 0032-3861
- DOI
- 10.1016/j.polymer.2008.02.009
- language
- English
- LU publication?
- yes
- id
- f47db3d4-ac94-4ea0-9b10-2b854c6b6138 (old id 1206596)
- date added to LUP
- 2016-04-01 13:53:49
- date last changed
- 2022-01-27 21:43:44
@article{f47db3d4-ac94-4ea0-9b10-2b854c6b6138,
abstract = {{Thin semitransparent gels were prepared by radical copolymerization of N-acryloyl-m-aminophenylboronic acid (NAAPBA) and acrylamide (AAm) taken in molar ratios from 8:92 to 16:84, respectively, in water. The gels were characterized by the content of immobilized NAAPBA and monomer conversion. Scanning electron microscopy revealed the micrometer size pores in the dried gels. The wet gels displayed a linear optical response to sugars with sensitivity decreasing in the series: D-fructose, D-galactose, D-glucose, D-mannose, N-acetyl-D-glucosamine in the sugar concentration range from 1 to 40-60 mM at pH 7.3. Cross-linking of the gels with N,N-methylene-bis-acrylamide decreased the strength of optical response. Specific binding capacity of a diol-containing dye Alizarin Red S in the gels at pH 7.0 coincided with the content of immobilized NAAPBA indicating the 1: 1 stoichiometry of the reaction and, therefore, good accessibility of the boronic acid ligands for water-soluble diols. Permeability of the gels was studied with a non-interacting dye Ethyl Orange exhibiting the pore diffusion coefficient of 1.4 x 10(-7) cm(2)/s. The rate of optical response of the gels to glucose was found to be determined by diffusion of sugar into the relatively thick gels (1 = 0.35-1 mm) with effective diffusion coefficients of 2 x 10(-7) cm(2)/S. In the thinner gels (l = 0.1 mm) the input of other kinetic processes, such as affinity binding or structural rearrangements of the gel, was noticeable. (c) 2008 Elsevier Ltd. All rights reserved.}},
author = {{Kuzimenkova, Marina and Ivanov, Alexander and Thammakhet, Chongdee and Mikhalovska, Lyubov I. and Galaev, Igor and Thavarungkul, Panote and Kanatharana, Proespichaya and Mattiasson, Bo}},
issn = {{0032-3861}},
keywords = {{boronic acid; hydrogels; sugar sensing}},
language = {{eng}},
number = {{6}},
pages = {{1444--1454}},
publisher = {{Elsevier}},
series = {{Polymer}},
title = {{Optical responses, permeability and diol-specific reactivity of thin polyacrylamide gels containing immobilized phenylboronic acid}},
url = {{http://dx.doi.org/10.1016/j.polymer.2008.02.009}},
doi = {{10.1016/j.polymer.2008.02.009}},
volume = {{49}},
year = {{2008}},
}