Investigation of sera from various species by using lectin affinity arrays and scanning ellipsometry
(2005) In Analytica Chimica Acta 530(2). p.167-171- Abstract
- Serum proteins of different species and of different human blood groups exhibit various protein glycosylation patterns. Sera from human, pig, sheep and guinea pig have been applied to a panel of eight different lectins immobilized on a gold wafer. The biorecognition has been evaluated with scanning ellipsometry and the two-dimensional matrices obtained have been treated with image analysis and MVDA for evaluation. The results showed a clear difference in protein binding pattern between the different species and thereby separation of the different sera could be made. Dendograms indicate that human and pig sera are the most related of the four different sera investigated.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/158724
- author
- Carlsson, J ; Mecklenburg, M ; Lundström, I ; Danielsson, Bengt LU and Winquist, F
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytica Chimica Acta
- volume
- 530
- issue
- 2
- pages
- 167 - 171
- publisher
- Elsevier
- external identifiers
-
- wos:000227192800001
- scopus:13444261098
- ISSN
- 1873-4324
- DOI
- 10.1016/j.aca.2004.09.022
- language
- English
- LU publication?
- yes
- id
- f488d5ec-9c05-4382-b6c9-101cc4fb3838 (old id 158724)
- date added to LUP
- 2016-04-01 15:28:32
- date last changed
- 2022-01-28 05:32:36
@article{f488d5ec-9c05-4382-b6c9-101cc4fb3838, abstract = {{Serum proteins of different species and of different human blood groups exhibit various protein glycosylation patterns. Sera from human, pig, sheep and guinea pig have been applied to a panel of eight different lectins immobilized on a gold wafer. The biorecognition has been evaluated with scanning ellipsometry and the two-dimensional matrices obtained have been treated with image analysis and MVDA for evaluation. The results showed a clear difference in protein binding pattern between the different species and thereby separation of the different sera could be made. Dendograms indicate that human and pig sera are the most related of the four different sera investigated.}}, author = {{Carlsson, J and Mecklenburg, M and Lundström, I and Danielsson, Bengt and Winquist, F}}, issn = {{1873-4324}}, language = {{eng}}, number = {{2}}, pages = {{167--171}}, publisher = {{Elsevier}}, series = {{Analytica Chimica Acta}}, title = {{Investigation of sera from various species by using lectin affinity arrays and scanning ellipsometry}}, url = {{http://dx.doi.org/10.1016/j.aca.2004.09.022}}, doi = {{10.1016/j.aca.2004.09.022}}, volume = {{530}}, year = {{2005}}, }