Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Proteomic Profiling of Diffuse Large B-Cell Lymphomas

Kwiecińska, Anna LU ; Porwit, Anna LU ; Souchelnytskyi, Nazariy ; Kaufeldt, Ann ; Larsson, Catharina ; Bajalica-Lagercrantz, Svetlana and Souchelnytskyi, Serhiy (2018) In Pathobiology 85(4). p.211-219
Abstract

Objective: The aim of this study was to identify differences in proteome profiles of diffuse large B-cell lymphoma (DLBCL) of nongerminal center (non-GC) versus GC type in the search for new markers and drug targets. Methods: Six DLBCL, with 3 repeats for each, were used for the initial study by proteomics: 3 non-GC and 3 GC DLBCL cases. For immunohistochemistry, tissue microarrays were made from 31 DLBCL samples: 16 non-GC de novo lymphomas and 15 GC cases (11 transformed from follicular lymphomas and 4 de novo GC lymphomas). Proteome profiling was performed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. Results: Ninety-one proteins were found differentially expressed in non-GC compared to GC type. The... (More)

Objective: The aim of this study was to identify differences in proteome profiles of diffuse large B-cell lymphoma (DLBCL) of nongerminal center (non-GC) versus GC type in the search for new markers and drug targets. Methods: Six DLBCL, with 3 repeats for each, were used for the initial study by proteomics: 3 non-GC and 3 GC DLBCL cases. For immunohistochemistry, tissue microarrays were made from 31 DLBCL samples: 16 non-GC de novo lymphomas and 15 GC cases (11 transformed from follicular lymphomas and 4 de novo GC lymphomas). Proteome profiling was performed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. Results: Ninety-one proteins were found differentially expressed in non-GC compared to GC type. The Cytoscape tool was used for systemic analysis of proteomics data, revealing 19 subnetworks representing functions affected in non-GC versus GC types of DLBCL. Conclusion: A validation study of 3 selected proteins (BiP/Grp78, Hsp90, and cyclin B2) showed the enhanced expression in non-GC DLBCL, supporting the proteomics data.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
BiP/Grp78, Cyclin B2, De novo diffuse large B-cell lymphomas, Diffuse large B-cell lymphoma, Germinal center type, Hsp90, Non-germinal center type, Proteomics, Transformed lymphoma
in
Pathobiology
volume
85
issue
4
pages
211 - 219
publisher
Karger
external identifiers
  • scopus:85045049771
  • pmid:29617697
ISSN
1015-2008
DOI
10.1159/000486285
language
English
LU publication?
yes
id
f51f95bc-618d-4309-8e55-388b30c2440d
date added to LUP
2018-04-19 12:45:37
date last changed
2021-10-06 02:01:34
@article{f51f95bc-618d-4309-8e55-388b30c2440d,
  abstract     = {<p>Objective: The aim of this study was to identify differences in proteome profiles of diffuse large B-cell lymphoma (DLBCL) of nongerminal center (non-GC) versus GC type in the search for new markers and drug targets. Methods: Six DLBCL, with 3 repeats for each, were used for the initial study by proteomics: 3 non-GC and 3 GC DLBCL cases. For immunohistochemistry, tissue microarrays were made from 31 DLBCL samples: 16 non-GC de novo lymphomas and 15 GC cases (11 transformed from follicular lymphomas and 4 de novo GC lymphomas). Proteome profiling was performed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. Results: Ninety-one proteins were found differentially expressed in non-GC compared to GC type. The Cytoscape tool was used for systemic analysis of proteomics data, revealing 19 subnetworks representing functions affected in non-GC versus GC types of DLBCL. Conclusion: A validation study of 3 selected proteins (BiP/Grp78, Hsp90, and cyclin B2) showed the enhanced expression in non-GC DLBCL, supporting the proteomics data.</p>},
  author       = {Kwiecińska, Anna and Porwit, Anna and Souchelnytskyi, Nazariy and Kaufeldt, Ann and Larsson, Catharina and Bajalica-Lagercrantz, Svetlana and Souchelnytskyi, Serhiy},
  issn         = {1015-2008},
  language     = {eng},
  month        = {04},
  number       = {4},
  pages        = {211--219},
  publisher    = {Karger},
  series       = {Pathobiology},
  title        = {Proteomic Profiling of Diffuse Large B-Cell Lymphomas},
  url          = {http://dx.doi.org/10.1159/000486285},
  doi          = {10.1159/000486285},
  volume       = {85},
  year         = {2018},
}