Proteomic Profiling of Diffuse Large B-Cell Lymphomas
(2018) In Pathobiology 85(4). p.211-219- Abstract
Objective: The aim of this study was to identify differences in proteome profiles of diffuse large B-cell lymphoma (DLBCL) of nongerminal center (non-GC) versus GC type in the search for new markers and drug targets. Methods: Six DLBCL, with 3 repeats for each, were used for the initial study by proteomics: 3 non-GC and 3 GC DLBCL cases. For immunohistochemistry, tissue microarrays were made from 31 DLBCL samples: 16 non-GC de novo lymphomas and 15 GC cases (11 transformed from follicular lymphomas and 4 de novo GC lymphomas). Proteome profiling was performed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. Results: Ninety-one proteins were found differentially expressed in non-GC compared to GC type. The... (More)
Objective: The aim of this study was to identify differences in proteome profiles of diffuse large B-cell lymphoma (DLBCL) of nongerminal center (non-GC) versus GC type in the search for new markers and drug targets. Methods: Six DLBCL, with 3 repeats for each, were used for the initial study by proteomics: 3 non-GC and 3 GC DLBCL cases. For immunohistochemistry, tissue microarrays were made from 31 DLBCL samples: 16 non-GC de novo lymphomas and 15 GC cases (11 transformed from follicular lymphomas and 4 de novo GC lymphomas). Proteome profiling was performed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. Results: Ninety-one proteins were found differentially expressed in non-GC compared to GC type. The Cytoscape tool was used for systemic analysis of proteomics data, revealing 19 subnetworks representing functions affected in non-GC versus GC types of DLBCL. Conclusion: A validation study of 3 selected proteins (BiP/Grp78, Hsp90, and cyclin B2) showed the enhanced expression in non-GC DLBCL, supporting the proteomics data.
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- author
- Kwiecińska, Anna LU ; Porwit, Anna LU ; Souchelnytskyi, Nazariy ; Kaufeldt, Ann ; Larsson, Catharina ; Bajalica-Lagercrantz, Svetlana and Souchelnytskyi, Serhiy
- organization
- publishing date
- 2018-04-04
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- BiP/Grp78, Cyclin B2, De novo diffuse large B-cell lymphomas, Diffuse large B-cell lymphoma, Germinal center type, Hsp90, Non-germinal center type, Proteomics, Transformed lymphoma
- in
- Pathobiology
- volume
- 85
- issue
- 4
- pages
- 211 - 219
- publisher
- Karger
- external identifiers
-
- pmid:29617697
- scopus:85045049771
- ISSN
- 1015-2008
- DOI
- 10.1159/000486285
- language
- English
- LU publication?
- yes
- id
- f51f95bc-618d-4309-8e55-388b30c2440d
- date added to LUP
- 2018-04-19 12:45:37
- date last changed
- 2024-08-19 16:55:04
@article{f51f95bc-618d-4309-8e55-388b30c2440d, abstract = {{<p>Objective: The aim of this study was to identify differences in proteome profiles of diffuse large B-cell lymphoma (DLBCL) of nongerminal center (non-GC) versus GC type in the search for new markers and drug targets. Methods: Six DLBCL, with 3 repeats for each, were used for the initial study by proteomics: 3 non-GC and 3 GC DLBCL cases. For immunohistochemistry, tissue microarrays were made from 31 DLBCL samples: 16 non-GC de novo lymphomas and 15 GC cases (11 transformed from follicular lymphomas and 4 de novo GC lymphomas). Proteome profiling was performed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. Results: Ninety-one proteins were found differentially expressed in non-GC compared to GC type. The Cytoscape tool was used for systemic analysis of proteomics data, revealing 19 subnetworks representing functions affected in non-GC versus GC types of DLBCL. Conclusion: A validation study of 3 selected proteins (BiP/Grp78, Hsp90, and cyclin B2) showed the enhanced expression in non-GC DLBCL, supporting the proteomics data.</p>}}, author = {{Kwiecińska, Anna and Porwit, Anna and Souchelnytskyi, Nazariy and Kaufeldt, Ann and Larsson, Catharina and Bajalica-Lagercrantz, Svetlana and Souchelnytskyi, Serhiy}}, issn = {{1015-2008}}, keywords = {{BiP/Grp78; Cyclin B2; De novo diffuse large B-cell lymphomas; Diffuse large B-cell lymphoma; Germinal center type; Hsp90; Non-germinal center type; Proteomics; Transformed lymphoma}}, language = {{eng}}, month = {{04}}, number = {{4}}, pages = {{211--219}}, publisher = {{Karger}}, series = {{Pathobiology}}, title = {{Proteomic Profiling of Diffuse Large B-Cell Lymphomas}}, url = {{http://dx.doi.org/10.1159/000486285}}, doi = {{10.1159/000486285}}, volume = {{85}}, year = {{2018}}, }