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Quantification of Human Kallikrein-2 in Clinical Samples by Selected Reaction Monitoring

Végvári, Ákos LU ; Sjödin, Karin LU ; Rezeli, Melinda LU orcid and Marko-Varga, György LU (2013) In Journal of Proteome Research 12(10). p.4612-4616
Abstract
Recently, the number of mass spectrometry-based quantification assays has been increased, partly due to the global efforts of chromosome-centric human proteome project (C-HPP). Our goal at the Chromosome 19 Consortium is to provide novel selected reaction monitoring (SRM) assays of proteins coded on chromosome 19. We have selected the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR) of human kallikrein-2 (hK2 – NX_P20151) and developed an SRM assay. Details about the analytical parameters, including multiple transitions by peptides are presented. The endogenous levels of hK2 were determined in clinical samples (n=35). The limit of quantification was also estimated by spiking heavy isotope labeled peptides into seminal plasma... (More)
Recently, the number of mass spectrometry-based quantification assays has been increased, partly due to the global efforts of chromosome-centric human proteome project (C-HPP). Our goal at the Chromosome 19 Consortium is to provide novel selected reaction monitoring (SRM) assays of proteins coded on chromosome 19. We have selected the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR) of human kallikrein-2 (hK2 – NX_P20151) and developed an SRM assay. Details about the analytical parameters, including multiple transitions by peptides are presented. The endogenous levels of hK2 were determined in clinical samples (n=35). The limit of quantification was also estimated by spiking heavy isotope labeled peptides into seminal plasma samples at various concentrations (LOQ ≈ 29 ng/mL). (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Chromosome 19, Human kallikrein-2, Selected reaction monitoring, Mass spectrometry
in
Journal of Proteome Research
volume
12
issue
10
pages
4612 - 4616
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000326320300031
  • scopus:84885192152
  • pmid:23978317
ISSN
1535-3893
DOI
10.1021/pr400423k
language
English
LU publication?
yes
id
f51fddcd-78cb-44d6-be96-87b8fc9fb1c5 (old id 4002349)
date added to LUP
2016-04-01 10:47:20
date last changed
2023-08-31 11:28:34
@article{f51fddcd-78cb-44d6-be96-87b8fc9fb1c5,
  abstract     = {{Recently, the number of mass spectrometry-based quantification assays has been increased, partly due to the global efforts of chromosome-centric human proteome project (C-HPP). Our goal at the Chromosome 19 Consortium is to provide novel selected reaction monitoring (SRM) assays of proteins coded on chromosome 19. We have selected the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR) of human kallikrein-2 (hK2 – NX_P20151) and developed an SRM assay. Details about the analytical parameters, including multiple transitions by peptides are presented. The endogenous levels of hK2 were determined in clinical samples (n=35). The limit of quantification was also estimated by spiking heavy isotope labeled peptides into seminal plasma samples at various concentrations (LOQ ≈ 29 ng/mL).}},
  author       = {{Végvári, Ákos and Sjödin, Karin and Rezeli, Melinda and Marko-Varga, György}},
  issn         = {{1535-3893}},
  keywords     = {{Chromosome 19; Human kallikrein-2; Selected reaction monitoring; Mass spectrometry}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{4612--4616}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Journal of Proteome Research}},
  title        = {{Quantification of Human Kallikrein-2 in Clinical Samples by Selected Reaction Monitoring}},
  url          = {{http://dx.doi.org/10.1021/pr400423k}},
  doi          = {{10.1021/pr400423k}},
  volume       = {{12}},
  year         = {{2013}},
}