Quantification of Human Kallikrein-2 in Clinical Samples by Selected Reaction Monitoring
(2013) In Journal of Proteome Research 12(10). p.4612-4616- Abstract
- Recently, the number of mass spectrometry-based quantification assays has been increased, partly due to the global efforts of chromosome-centric human proteome project (C-HPP). Our goal at the Chromosome 19 Consortium is to provide novel selected reaction monitoring (SRM) assays of proteins coded on chromosome 19. We have selected the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR) of human kallikrein-2 (hK2 – NX_P20151) and developed an SRM assay. Details about the analytical parameters, including multiple transitions by peptides are presented. The endogenous levels of hK2 were determined in clinical samples (n=35). The limit of quantification was also estimated by spiking heavy isotope labeled peptides into seminal plasma... (More)
- Recently, the number of mass spectrometry-based quantification assays has been increased, partly due to the global efforts of chromosome-centric human proteome project (C-HPP). Our goal at the Chromosome 19 Consortium is to provide novel selected reaction monitoring (SRM) assays of proteins coded on chromosome 19. We have selected the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR) of human kallikrein-2 (hK2 – NX_P20151) and developed an SRM assay. Details about the analytical parameters, including multiple transitions by peptides are presented. The endogenous levels of hK2 were determined in clinical samples (n=35). The limit of quantification was also estimated by spiking heavy isotope labeled peptides into seminal plasma samples at various concentrations (LOQ ≈ 29 ng/mL). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4002349
- author
- Végvári, Ákos LU ; Sjödin, Karin LU ; Rezeli, Melinda LU and Marko-Varga, György LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Chromosome 19, Human kallikrein-2, Selected reaction monitoring, Mass spectrometry
- in
- Journal of Proteome Research
- volume
- 12
- issue
- 10
- pages
- 4612 - 4616
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000326320300031
- scopus:84885192152
- pmid:23978317
- ISSN
- 1535-3893
- DOI
- 10.1021/pr400423k
- language
- English
- LU publication?
- yes
- id
- f51fddcd-78cb-44d6-be96-87b8fc9fb1c5 (old id 4002349)
- date added to LUP
- 2016-04-01 10:47:20
- date last changed
- 2023-08-31 11:28:34
@article{f51fddcd-78cb-44d6-be96-87b8fc9fb1c5, abstract = {{Recently, the number of mass spectrometry-based quantification assays has been increased, partly due to the global efforts of chromosome-centric human proteome project (C-HPP). Our goal at the Chromosome 19 Consortium is to provide novel selected reaction monitoring (SRM) assays of proteins coded on chromosome 19. We have selected the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR) of human kallikrein-2 (hK2 – NX_P20151) and developed an SRM assay. Details about the analytical parameters, including multiple transitions by peptides are presented. The endogenous levels of hK2 were determined in clinical samples (n=35). The limit of quantification was also estimated by spiking heavy isotope labeled peptides into seminal plasma samples at various concentrations (LOQ ≈ 29 ng/mL).}}, author = {{Végvári, Ákos and Sjödin, Karin and Rezeli, Melinda and Marko-Varga, György}}, issn = {{1535-3893}}, keywords = {{Chromosome 19; Human kallikrein-2; Selected reaction monitoring; Mass spectrometry}}, language = {{eng}}, number = {{10}}, pages = {{4612--4616}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Journal of Proteome Research}}, title = {{Quantification of Human Kallikrein-2 in Clinical Samples by Selected Reaction Monitoring}}, url = {{http://dx.doi.org/10.1021/pr400423k}}, doi = {{10.1021/pr400423k}}, volume = {{12}}, year = {{2013}}, }