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An UPLC-MS/MS assay to measure glutathione as marker for oxidative stress in cultured cells

Herzog, Katharina LU ; Ijlst, Lodewijk ; van Cruchten, Arno G. ; van Roermund, Carlo W.T. ; Kulik, Wim ; Wanders, Ronald J.A. and Waterham, Hans R. (2019) In Metabolites 9(3).
Abstract

Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cultured cells, Glutathione, Mass spectrometry, Oxidative stress, Yeast
in
Metabolites
volume
9
issue
3
article number
45
publisher
MDPI AG
external identifiers
  • scopus:85064521253
  • pmid:30841653
ISSN
2218-1989
DOI
10.3390/metabo9030045
language
English
LU publication?
yes
id
f6122b19-8ed6-4862-b2b0-e511fddebf73
date added to LUP
2019-05-06 10:20:12
date last changed
2024-05-29 07:55:57
@article{f6122b19-8ed6-4862-b2b0-e511fddebf73,
  abstract     = {{<p>Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).</p>}},
  author       = {{Herzog, Katharina and Ijlst, Lodewijk and van Cruchten, Arno G. and van Roermund, Carlo W.T. and Kulik, Wim and Wanders, Ronald J.A. and Waterham, Hans R.}},
  issn         = {{2218-1989}},
  keywords     = {{Cultured cells; Glutathione; Mass spectrometry; Oxidative stress; Yeast}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{3}},
  publisher    = {{MDPI AG}},
  series       = {{Metabolites}},
  title        = {{An UPLC-MS/MS assay to measure glutathione as marker for oxidative stress in cultured cells}},
  url          = {{http://dx.doi.org/10.3390/metabo9030045}},
  doi          = {{10.3390/metabo9030045}},
  volume       = {{9}},
  year         = {{2019}},
}