An UPLC-MS/MS assay to measure glutathione as marker for oxidative stress in cultured cells
(2019) In Metabolites 9(3).- Abstract
Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/f6122b19-8ed6-4862-b2b0-e511fddebf73
- author
- Herzog, Katharina LU ; Ijlst, Lodewijk ; van Cruchten, Arno G. ; van Roermund, Carlo W.T. ; Kulik, Wim ; Wanders, Ronald J.A. and Waterham, Hans R.
- organization
- publishing date
- 2019-03-05
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cultured cells, Glutathione, Mass spectrometry, Oxidative stress, Yeast
- in
- Metabolites
- volume
- 9
- issue
- 3
- article number
- 45
- publisher
- MDPI AG
- external identifiers
-
- pmid:30841653
- scopus:85064521253
- ISSN
- 2218-1989
- DOI
- 10.3390/metabo9030045
- language
- English
- LU publication?
- yes
- id
- f6122b19-8ed6-4862-b2b0-e511fddebf73
- date added to LUP
- 2019-05-06 10:20:12
- date last changed
- 2024-09-17 19:12:20
@article{f6122b19-8ed6-4862-b2b0-e511fddebf73, abstract = {{<p>Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).</p>}}, author = {{Herzog, Katharina and Ijlst, Lodewijk and van Cruchten, Arno G. and van Roermund, Carlo W.T. and Kulik, Wim and Wanders, Ronald J.A. and Waterham, Hans R.}}, issn = {{2218-1989}}, keywords = {{Cultured cells; Glutathione; Mass spectrometry; Oxidative stress; Yeast}}, language = {{eng}}, month = {{03}}, number = {{3}}, publisher = {{MDPI AG}}, series = {{Metabolites}}, title = {{An UPLC-MS/MS assay to measure glutathione as marker for oxidative stress in cultured cells}}, url = {{http://dx.doi.org/10.3390/metabo9030045}}, doi = {{10.3390/metabo9030045}}, volume = {{9}}, year = {{2019}}, }