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Molecular engineering of biotin-glutamic acid decarboxylase 65 fusion protein (Biotin-GAD65) for non-radioactive GAD65 antibody assay

Luo, Dong ; Rogers, Chad N ; Hampe, Christiane S and Lernmark, Åke LU orcid (2003) In Journal of Biotechnology 103(3). p.249-255
Abstract
We constructed biotinylated fusion proteins that linked to three detection tags to GAD65 at the N-terminus, and expressed them in an E. coli expression system. The Biotin14-GAD65 protein exhibited the strongest binding to both the GAD65 antibody and the streptavidin among the three constructs. We describe the optimal conditions using a Biotin14-GAD65-based immunoassay for the detection of GAD65 antibody.
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author
; ; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biotechnology
volume
103
issue
3
pages
249 - 255
publisher
Elsevier
external identifiers
  • scopus:0142059622
ISSN
1873-4863
DOI
10.1016/S0168-1656(03)00125-1
language
English
LU publication?
no
id
f635666b-9de3-47d5-9709-55f44b726f5d (old id 1127400)
date added to LUP
2016-04-01 11:56:33
date last changed
2025-10-14 12:56:42
@article{f635666b-9de3-47d5-9709-55f44b726f5d,
  abstract     = {{We constructed biotinylated fusion proteins that linked to three detection tags to GAD65 at the N-terminus, and expressed them in an E. coli expression system. The Biotin14-GAD65 protein exhibited the strongest binding to both the GAD65 antibody and the streptavidin among the three constructs. We describe the optimal conditions using a Biotin14-GAD65-based immunoassay for the detection of GAD65 antibody.}},
  author       = {{Luo, Dong and Rogers, Chad N and Hampe, Christiane S and Lernmark, Åke}},
  issn         = {{1873-4863}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{249--255}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Biotechnology}},
  title        = {{Molecular engineering of biotin-glutamic acid decarboxylase 65 fusion protein (Biotin-GAD65) for non-radioactive GAD65 antibody assay}},
  url          = {{http://dx.doi.org/10.1016/S0168-1656(03)00125-1}},
  doi          = {{10.1016/S0168-1656(03)00125-1}},
  volume       = {{103}},
  year         = {{2003}},
}