Molecular engineering of biotin-glutamic acid decarboxylase 65 fusion protein (Biotin-GAD65) for non-radioactive GAD65 antibody assay
(2003) In Journal of Biotechnology 103(3). p.249-255- Abstract
- We constructed biotinylated fusion proteins that linked to three detection tags to GAD65 at the N-terminus, and expressed them in an E. coli expression system. The Biotin14-GAD65 protein exhibited the strongest binding to both the GAD65 antibody and the streptavidin among the three constructs. We describe the optimal conditions using a Biotin14-GAD65-based immunoassay for the detection of GAD65 antibody.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1127400
- author
- Luo, Dong ; Rogers, Chad N ; Hampe, Christiane S and Lernmark, Åke LU
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biotechnology
- volume
- 103
- issue
- 3
- pages
- 249 - 255
- publisher
- Elsevier
- external identifiers
-
- scopus:0142059622
- ISSN
- 1873-4863
- DOI
- 10.1016/S0168-1656(03)00125-1
- language
- English
- LU publication?
- no
- id
- f635666b-9de3-47d5-9709-55f44b726f5d (old id 1127400)
- date added to LUP
- 2016-04-01 11:56:33
- date last changed
- 2022-01-26 20:32:46
@article{f635666b-9de3-47d5-9709-55f44b726f5d, abstract = {{We constructed biotinylated fusion proteins that linked to three detection tags to GAD65 at the N-terminus, and expressed them in an E. coli expression system. The Biotin14-GAD65 protein exhibited the strongest binding to both the GAD65 antibody and the streptavidin among the three constructs. We describe the optimal conditions using a Biotin14-GAD65-based immunoassay for the detection of GAD65 antibody.}}, author = {{Luo, Dong and Rogers, Chad N and Hampe, Christiane S and Lernmark, Åke}}, issn = {{1873-4863}}, language = {{eng}}, number = {{3}}, pages = {{249--255}}, publisher = {{Elsevier}}, series = {{Journal of Biotechnology}}, title = {{Molecular engineering of biotin-glutamic acid decarboxylase 65 fusion protein (Biotin-GAD65) for non-radioactive GAD65 antibody assay}}, url = {{http://dx.doi.org/10.1016/S0168-1656(03)00125-1}}, doi = {{10.1016/S0168-1656(03)00125-1}}, volume = {{103}}, year = {{2003}}, }