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Development of a carbon nanotube paste electrode osmium polymer-mediated biosensor for determination of glucose in alcoholic beverages

Antiochia, Riccarda and Gorton, Lo LU (2007) In Biosensors & Bioelectronics 22(11). p.2611-2617
Abstract
This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2 V versus Ag vertical bar AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 mu mol L-1, linearity up to 8 x 10(-4) mol L-1, a sensitivity of 13.4 mu A cm(-2) mmol(-1) L-1, a good... (More)
This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2 V versus Ag vertical bar AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 mu mol L-1, linearity up to 8 x 10(-4) mol L-1, a sensitivity of 13.4 mu A cm(-2) mmol(-1) L-1, a good reproducibility (R.S.D. 2.1%, n = 6) and a stability of about 1 week when stored dry at 4 degrees C. Finally, the proposed biosensor was applied for the determination of glucose in different samples of sweet wine and validated with a commercial spectrophotometric enzymatic kit. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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Contribution to journal
publication status
published
subject
keywords
osmium redox, diaphorase, glucose dehydrogenase, biosensor, glucose, polymer, carbon nanotube paste electrode
in
Biosensors & Bioelectronics
volume
22
issue
11
pages
2611 - 2617
publisher
Elsevier
external identifiers
  • wos:000246382500031
  • scopus:33947584425
ISSN
1873-4235
DOI
10.1016/j.bios.2006.10.023
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
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f635f056-535c-4538-87cd-f9b939bab7c7 (old id 660590)
date added to LUP
2016-04-01 16:24:55
date last changed
2022-03-15 00:23:43
@article{f635f056-535c-4538-87cd-f9b939bab7c7,
  abstract     = {{This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2 V versus Ag vertical bar AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 mu mol L-1, linearity up to 8 x 10(-4) mol L-1, a sensitivity of 13.4 mu A cm(-2) mmol(-1) L-1, a good reproducibility (R.S.D. 2.1%, n = 6) and a stability of about 1 week when stored dry at 4 degrees C. Finally, the proposed biosensor was applied for the determination of glucose in different samples of sweet wine and validated with a commercial spectrophotometric enzymatic kit. (c) 2006 Elsevier B.V. All rights reserved.}},
  author       = {{Antiochia, Riccarda and Gorton, Lo}},
  issn         = {{1873-4235}},
  keywords     = {{osmium redox; diaphorase; glucose dehydrogenase; biosensor; glucose; polymer; carbon nanotube paste electrode}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{2611--2617}},
  publisher    = {{Elsevier}},
  series       = {{Biosensors & Bioelectronics}},
  title        = {{Development of a carbon nanotube paste electrode osmium polymer-mediated biosensor for determination of glucose in alcoholic beverages}},
  url          = {{http://dx.doi.org/10.1016/j.bios.2006.10.023}},
  doi          = {{10.1016/j.bios.2006.10.023}},
  volume       = {{22}},
  year         = {{2007}},
}