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Molecular characterisation of three avian haemoproteids (Haemosporida, Haemoproteidae), with the description of Haemoproteus (Parahaemoproteus) palloris n. sp.

Dimitrov, Dimitar; Iezhova, Tatjana A.; Zehtindjiev, Pavel; Bobeva, Aneliya; Ilieva, Mihaela LU ; Kirilova, Miroslava; Bedev, Kiril; Sjöholm, Christoffer and Valkiūnas, Gediminas (2016) In Systematic Parasitology 93(5). p.431-449
Abstract

DNA barcoding (molecular characterisation) is a useful tool for describing the taxonomy and systematics of organisms. Over 250 species of avian haemosporidian parasites have been described using morphological characters, yet molecular techniques based on polymerase chain reaction (PCR) suggest this diversity is underestimated. Moreover, molecular techniques are particularly useful for the detection of chronic infections and tissue stages of these parasites. Species delimitation is problematic among haemosporidians, and many questions about the mechanisms and patterns of speciation, host specificity and pathogenicity are still unresolved. Accumulation of additional genetic and morphological information is needed to approach these... (More)

DNA barcoding (molecular characterisation) is a useful tool for describing the taxonomy and systematics of organisms. Over 250 species of avian haemosporidian parasites have been described using morphological characters, yet molecular techniques based on polymerase chain reaction (PCR) suggest this diversity is underestimated. Moreover, molecular techniques are particularly useful for the detection of chronic infections and tissue stages of these parasites. Species delimitation is problematic among haemosporidians, and many questions about the mechanisms and patterns of speciation, host specificity and pathogenicity are still unresolved. Accumulation of additional genetic and morphological information is needed to approach these questions. Here, we combine microscopic examination with PCR-based methods to develop molecular characterisation of Haemoproteus (Parahaemoproteus) manwelli Bennett, 1978 and Haemoproteus (Parahaemoproteus) gavrilovi Valkiūnas & Iezhova, 1990, both of which parasitise the bee-eater Merops apiaster L. We also describe a new species, Haemoproteus (Parahaemoproteus) palloris n. sp., from the blood of the willow warbler Phylloscopus trochilus (L.). We performed phylogenetic analyses with a set of mitochondrial cytochrome b (cyt b) gene lineages, which have been linked to parasite morphospecies and are available in the MalAvi database. Our findings show that morphological characters, which have been traditionally used in the description of haemosporidians, exhibit phylogenetic congruence. This study contributes to a better understanding of avian haemosporidian diversity and provides new molecular markers (cyt b and apicoplast gene sequences) for the diagnostics of inadequately investigated haemosporidian infections.

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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Systematic Parasitology
volume
93
issue
5
pages
19 pages
publisher
Springer Netherlands
external identifiers
  • scopus:84971659921
ISSN
0165-5752
DOI
10.1007/s11230-016-9638-8
language
English
LU publication?
no
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f6b74eec-0bf8-476e-ac2d-f568ab0ea824
date added to LUP
2017-01-25 14:42:37
date last changed
2017-03-19 04:39:28
@article{f6b74eec-0bf8-476e-ac2d-f568ab0ea824,
  abstract     = {<p>DNA barcoding (molecular characterisation) is a useful tool for describing the taxonomy and systematics of organisms. Over 250 species of avian haemosporidian parasites have been described using morphological characters, yet molecular techniques based on polymerase chain reaction (PCR) suggest this diversity is underestimated. Moreover, molecular techniques are particularly useful for the detection of chronic infections and tissue stages of these parasites. Species delimitation is problematic among haemosporidians, and many questions about the mechanisms and patterns of speciation, host specificity and pathogenicity are still unresolved. Accumulation of additional genetic and morphological information is needed to approach these questions. Here, we combine microscopic examination with PCR-based methods to develop molecular characterisation of Haemoproteus (Parahaemoproteus) manwelli Bennett, 1978 and Haemoproteus (Parahaemoproteus) gavrilovi Valkiūnas &amp; Iezhova, 1990, both of which parasitise the bee-eater Merops apiaster L. We also describe a new species, Haemoproteus (Parahaemoproteus) palloris n. sp., from the blood of the willow warbler Phylloscopus trochilus (L.). We performed phylogenetic analyses with a set of mitochondrial cytochrome b (cyt b) gene lineages, which have been linked to parasite morphospecies and are available in the MalAvi database. Our findings show that morphological characters, which have been traditionally used in the description of haemosporidians, exhibit phylogenetic congruence. This study contributes to a better understanding of avian haemosporidian diversity and provides new molecular markers (cyt b and apicoplast gene sequences) for the diagnostics of inadequately investigated haemosporidian infections.</p>},
  author       = {Dimitrov, Dimitar and Iezhova, Tatjana A. and Zehtindjiev, Pavel and Bobeva, Aneliya and Ilieva, Mihaela and Kirilova, Miroslava and Bedev, Kiril and Sjöholm, Christoffer and Valkiūnas, Gediminas},
  issn         = {0165-5752},
  language     = {eng},
  month        = {06},
  number       = {5},
  pages        = {431--449},
  publisher    = {Springer Netherlands},
  series       = {Systematic Parasitology},
  title        = {Molecular characterisation of three avian haemoproteids (Haemosporida, Haemoproteidae), with the description of Haemoproteus (Parahaemoproteus) palloris n. sp.},
  url          = {http://dx.doi.org/10.1007/s11230-016-9638-8},
  volume       = {93},
  year         = {2016},
}