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Rapid separation of CD4+ and CD19+ lymphocyte populations from human peripheral blood by a magnetic activated cell sorter (MACS)

Semple, John W. LU ; Allen, David ; Chang, Wilda ; Castaldi, Patrick and Freedman, John (1993) In Cytometry 14(8). p.955-960
Abstract

Rapid purification of human lymphocyte subpopulations is an essential step in order to elucidate their interactions and/or contributions in various disease states. Cell purification using a Magnetic Activated Cell Sorter (MACS) is a relatively new technology which has been shown to be rapid and yield highly purified populations of cells. This report describes both a simple one‐step positive selection method using the MACS to purify either human CD4+ or CD19+ lymphocytes from PBMC and a sequential separation of both CD4+ and CD19+ cell populations. These methods can separate the cell populations in approximately 4 h with yields >90% and purity of 97 ± 3% for CD4+ T cells and 92 ± 5% for CD19+ B cells. In functional studies, purified... (More)

Rapid purification of human lymphocyte subpopulations is an essential step in order to elucidate their interactions and/or contributions in various disease states. Cell purification using a Magnetic Activated Cell Sorter (MACS) is a relatively new technology which has been shown to be rapid and yield highly purified populations of cells. This report describes both a simple one‐step positive selection method using the MACS to purify either human CD4+ or CD19+ lymphocytes from PBMC and a sequential separation of both CD4+ and CD19+ cell populations. These methods can separate the cell populations in approximately 4 h with yields >90% and purity of 97 ± 3% for CD4+ T cells and 92 ± 5% for CD19+ B cells. In functional studies, purified CD19+ B cells secreted 13‐ and 24‐fold more IgM and IgG, respectively, than the CD19− cell fraction in 10 day B cell stimulation assays. Purification of the two cell types did not cause any significant activation as shown by proliferation. Both cell types, however, were able to proliferate upon stimulation with interleukin‐2. © 1993 Wiley‐Liss, Inc.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
B lymphocytes, Magnetic activated cell sorter, positive selection, T lymphocytes
in
Cytometry
volume
14
issue
8
pages
955 - 960
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:0027358497
  • pmid:7507026
ISSN
0196-4763
DOI
10.1002/cyto.990140816
language
English
LU publication?
no
id
f6cdd3a0-20a4-42f9-bd1a-da802559ec26
date added to LUP
2019-12-03 10:34:16
date last changed
2024-01-02 01:34:09
@article{f6cdd3a0-20a4-42f9-bd1a-da802559ec26,
  abstract     = {{<p>Rapid purification of human lymphocyte subpopulations is an essential step in order to elucidate their interactions and/or contributions in various disease states. Cell purification using a Magnetic Activated Cell Sorter (MACS) is a relatively new technology which has been shown to be rapid and yield highly purified populations of cells. This report describes both a simple one‐step positive selection method using the MACS to purify either human CD4+ or CD19+ lymphocytes from PBMC and a sequential separation of both CD4+ and CD19+ cell populations. These methods can separate the cell populations in approximately 4 h with yields &gt;90% and purity of 97 ± 3% for CD4+ T cells and 92 ± 5% for CD19+ B cells. In functional studies, purified CD19+ B cells secreted 13‐ and 24‐fold more IgM and IgG, respectively, than the CD19− cell fraction in 10 day B cell stimulation assays. Purification of the two cell types did not cause any significant activation as shown by proliferation. Both cell types, however, were able to proliferate upon stimulation with interleukin‐2. © 1993 Wiley‐Liss, Inc.</p>}},
  author       = {{Semple, John W. and Allen, David and Chang, Wilda and Castaldi, Patrick and Freedman, John}},
  issn         = {{0196-4763}},
  keywords     = {{B lymphocytes; Magnetic activated cell sorter; positive selection; T lymphocytes}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{8}},
  pages        = {{955--960}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Cytometry}},
  title        = {{Rapid separation of CD4+ and CD19+ lymphocyte populations from human peripheral blood by a magnetic activated cell sorter (MACS)}},
  url          = {{http://dx.doi.org/10.1002/cyto.990140816}},
  doi          = {{10.1002/cyto.990140816}},
  volume       = {{14}},
  year         = {{1993}},
}