In vitro maintenance of multipotent neural crest stem cells as crestospheres
Mohlin, Sofie LU
and Kerosuo, Laura
(2019)
In Methods in Molecular Biology
2002.
p.1-11
- Abstract
Neural crest cells are a critical source of many cell types of the vertebrate body. However, as a stem cell population they are peculiar because of the transient nature of their stem cell niche; soon after the multipotent neural crest cells are specified in the neuroepithelium, they become mesenchymal cells that migrate into various destinations in early embryos. These rapid in vivo changes during neural crest development complicate the studies on their stem cell properties. Crestospheres are in vitro maintained primary cultures of premigratory neural crest cells that maintain a mixture of neural crest stem and progenitor cells for weeks without spontaneous differentiation, including the multipotent neural crest stem cells. Here, we... (More)
Neural crest cells are a critical source of many cell types of the vertebrate body. However, as a stem cell population they are peculiar because of the transient nature of their stem cell niche; soon after the multipotent neural crest cells are specified in the neuroepithelium, they become mesenchymal cells that migrate into various destinations in early embryos. These rapid in vivo changes during neural crest development complicate the studies on their stem cell properties. Crestospheres are in vitro maintained primary cultures of premigratory neural crest cells that maintain a mixture of neural crest stem and progenitor cells for weeks without spontaneous differentiation, including the multipotent neural crest stem cells. Here, we describe how crestosphere cultures are initiated from either cranial or trunk levels of chick embryos. Alternatively, the same culture conditions can be used to maintain human embryonic stem cell-derived neural crest cells as crestospheres. Thus, crestospheres provide a useful tool for studies on neural crest stemness.
(Less)
- author
- Mohlin, Sofie
LU
and Kerosuo, Laura
- organization
- publishing date
- 2019
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Chick embryo, Cranial neural crest, Crestospheres, Human ES cell-derived neural crest, Multipotency, Neural crest, Self-renewal, Trunk neural crest
- host publication
- Methods in Molecular Biology
- series title
- Methods in Molecular Biology
- volume
- 2002
- pages
- 11 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:30159826
- scopus:85067038648
- ISSN
- 1064-3745
- ISBN
- 978-1-4939-9507-3
- DOI
- 10.1007/7651_2018_180
- language
- English
- LU publication?
- yes
- id
- f6d9ceeb-0ae1-448d-abdf-417b1e3bfc58
- date added to LUP
- 2019-07-03 10:08:53
- date last changed
- 2024-05-28 20:09:02
@inbook{f6d9ceeb-0ae1-448d-abdf-417b1e3bfc58,
abstract = {{<p>Neural crest cells are a critical source of many cell types of the vertebrate body. However, as a stem cell population they are peculiar because of the transient nature of their stem cell niche; soon after the multipotent neural crest cells are specified in the neuroepithelium, they become mesenchymal cells that migrate into various destinations in early embryos. These rapid in vivo changes during neural crest development complicate the studies on their stem cell properties. Crestospheres are in vitro maintained primary cultures of premigratory neural crest cells that maintain a mixture of neural crest stem and progenitor cells for weeks without spontaneous differentiation, including the multipotent neural crest stem cells. Here, we describe how crestosphere cultures are initiated from either cranial or trunk levels of chick embryos. Alternatively, the same culture conditions can be used to maintain human embryonic stem cell-derived neural crest cells as crestospheres. Thus, crestospheres provide a useful tool for studies on neural crest stemness.</p>}},
author = {{Mohlin, Sofie and Kerosuo, Laura}},
booktitle = {{Methods in Molecular Biology}},
isbn = {{978-1-4939-9507-3}},
issn = {{1064-3745}},
keywords = {{Chick embryo; Cranial neural crest; Crestospheres; Human ES cell-derived neural crest; Multipotency; Neural crest; Self-renewal; Trunk neural crest}},
language = {{eng}},
pages = {{1--11}},
publisher = {{Humana Press}},
series = {{Methods in Molecular Biology}},
title = {{In vitro maintenance of multipotent neural crest stem cells as crestospheres}},
url = {{http://dx.doi.org/10.1007/7651_2018_180}},
doi = {{10.1007/7651_2018_180}},
volume = {{2002}},
year = {{2019}},
}