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In vitro maintenance of multipotent neural crest stem cells as crestospheres

Mohlin, Sofie LU and Kerosuo, Laura (2019) In Methods in Molecular Biology 2002. p.1-11
Abstract

Neural crest cells are a critical source of many cell types of the vertebrate body. However, as a stem cell population they are peculiar because of the transient nature of their stem cell niche; soon after the multipotent neural crest cells are specified in the neuroepithelium, they become mesenchymal cells that migrate into various destinations in early embryos. These rapid in vivo changes during neural crest development complicate the studies on their stem cell properties. Crestospheres are in vitro maintained primary cultures of premigratory neural crest cells that maintain a mixture of neural crest stem and progenitor cells for weeks without spontaneous differentiation, including the multipotent neural crest stem cells. Here, we... (More)

Neural crest cells are a critical source of many cell types of the vertebrate body. However, as a stem cell population they are peculiar because of the transient nature of their stem cell niche; soon after the multipotent neural crest cells are specified in the neuroepithelium, they become mesenchymal cells that migrate into various destinations in early embryos. These rapid in vivo changes during neural crest development complicate the studies on their stem cell properties. Crestospheres are in vitro maintained primary cultures of premigratory neural crest cells that maintain a mixture of neural crest stem and progenitor cells for weeks without spontaneous differentiation, including the multipotent neural crest stem cells. Here, we describe how crestosphere cultures are initiated from either cranial or trunk levels of chick embryos. Alternatively, the same culture conditions can be used to maintain human embryonic stem cell-derived neural crest cells as crestospheres. Thus, crestospheres provide a useful tool for studies on neural crest stemness.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Chick embryo, Cranial neural crest, Crestospheres, Human ES cell-derived neural crest, Multipotency, Neural crest, Self-renewal, Trunk neural crest
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
2002
pages
11 pages
publisher
Humana Press
external identifiers
  • scopus:85067038648
ISSN
1064-3745
ISBN
978-1-4939-9507-3
DOI
10.1007/7651_2018_180
language
English
LU publication?
yes
id
f6d9ceeb-0ae1-448d-abdf-417b1e3bfc58
date added to LUP
2019-07-03 10:08:53
date last changed
2019-11-13 05:38:28
@inbook{f6d9ceeb-0ae1-448d-abdf-417b1e3bfc58,
  abstract     = {<p>Neural crest cells are a critical source of many cell types of the vertebrate body. However, as a stem cell population they are peculiar because of the transient nature of their stem cell niche; soon after the multipotent neural crest cells are specified in the neuroepithelium, they become mesenchymal cells that migrate into various destinations in early embryos. These rapid in vivo changes during neural crest development complicate the studies on their stem cell properties. Crestospheres are in vitro maintained primary cultures of premigratory neural crest cells that maintain a mixture of neural crest stem and progenitor cells for weeks without spontaneous differentiation, including the multipotent neural crest stem cells. Here, we describe how crestosphere cultures are initiated from either cranial or trunk levels of chick embryos. Alternatively, the same culture conditions can be used to maintain human embryonic stem cell-derived neural crest cells as crestospheres. Thus, crestospheres provide a useful tool for studies on neural crest stemness.</p>},
  author       = {Mohlin, Sofie and Kerosuo, Laura},
  isbn         = {978-1-4939-9507-3},
  issn         = {1064-3745},
  keyword      = {Chick embryo,Cranial neural crest,Crestospheres,Human ES cell-derived neural crest,Multipotency,Neural crest,Self-renewal,Trunk neural crest},
  language     = {eng},
  pages        = {1--11},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {In vitro maintenance of multipotent neural crest stem cells as crestospheres},
  url          = {http://dx.doi.org/10.1007/7651_2018_180},
  volume       = {2002},
  year         = {2019},
}