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Multiplex protein detection on circulating tumor cells from liquid biopsies using imaging mass cytometry

Gerdtsson, Erik ; Pore, Milind ; Thiele, Jana-Aletta ; Gerdtsson, Anna Sandström LU ; Malihi, Paymaneh D ; Nevarez, Rafael ; Kolatkar, Anand ; Velasco, Carmen Ruiz ; Wix, Sophia and Singh, Mohan , et al. (2018) In Convergent science physical oncology 4(1).
Abstract

Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation of prognosis and treatment efficacy in near-real time through minimally invasive liquid biopsies. To realize this potential, however, methods for molecular analysis of these rare cells must be developed and validated. Here, we describe the integration of imaging mass cytometry (IMC) using metal-labeled antibodies as implemented on the Fluidigm Hyperion Imaging System into the workflow of the previously established High Definition Single Cell Analysis (HD-SCA) assay for liquid biopsies, along with methods for image analysis and signal normalization. Using liquid biopsies from a metastatic prostate cancer... (More)

Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation of prognosis and treatment efficacy in near-real time through minimally invasive liquid biopsies. To realize this potential, however, methods for molecular analysis of these rare cells must be developed and validated. Here, we describe the integration of imaging mass cytometry (IMC) using metal-labeled antibodies as implemented on the Fluidigm Hyperion Imaging System into the workflow of the previously established High Definition Single Cell Analysis (HD-SCA) assay for liquid biopsies, along with methods for image analysis and signal normalization. Using liquid biopsies from a metastatic prostate cancer case, we demonstrate that IMC can extend the reach of CTC characterization to include dozens of protein biomarkers, with the potential to understand a range of biological properties that could affect therapeutic response, metastasis and immune surveillance when coupled with simultaneous phenotyping of thousands of leukocytes.

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publishing date
type
Contribution to journal
publication status
published
subject
in
Convergent science physical oncology
volume
4
issue
1
external identifiers
  • pmid:30906572
ISSN
2057-1739
DOI
10.1088/2057-1739/aaa013
language
English
LU publication?
no
id
f6eec3c9-12d2-41e5-95b1-f3af6e9ea11d
date added to LUP
2020-03-09 12:43:22
date last changed
2020-03-10 14:18:29
@article{f6eec3c9-12d2-41e5-95b1-f3af6e9ea11d,
  abstract     = {{<p>Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation of prognosis and treatment efficacy in near-real time through minimally invasive liquid biopsies. To realize this potential, however, methods for molecular analysis of these rare cells must be developed and validated. Here, we describe the integration of imaging mass cytometry (IMC) using metal-labeled antibodies as implemented on the Fluidigm Hyperion Imaging System into the workflow of the previously established High Definition Single Cell Analysis (HD-SCA) assay for liquid biopsies, along with methods for image analysis and signal normalization. Using liquid biopsies from a metastatic prostate cancer case, we demonstrate that IMC can extend the reach of CTC characterization to include dozens of protein biomarkers, with the potential to understand a range of biological properties that could affect therapeutic response, metastasis and immune surveillance when coupled with simultaneous phenotyping of thousands of leukocytes.</p>}},
  author       = {{Gerdtsson, Erik and Pore, Milind and Thiele, Jana-Aletta and Gerdtsson, Anna Sandström and Malihi, Paymaneh D and Nevarez, Rafael and Kolatkar, Anand and Velasco, Carmen Ruiz and Wix, Sophia and Singh, Mohan and Carlsson, Anders and Zurita, Amado J and Logothetis, Christopher and Merchant, Akil A and Hicks, James and Kuhn, Peter}},
  issn         = {{2057-1739}},
  language     = {{eng}},
  number       = {{1}},
  series       = {{Convergent science physical oncology}},
  title        = {{Multiplex protein detection on circulating tumor cells from liquid biopsies using imaging mass cytometry}},
  url          = {{http://dx.doi.org/10.1088/2057-1739/aaa013}},
  doi          = {{10.1088/2057-1739/aaa013}},
  volume       = {{4}},
  year         = {{2018}},
}