Multiplex protein detection on circulating tumor cells from liquid biopsies using imaging mass cytometry
(2018) In Convergent science physical oncology 4(1).- Abstract
Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation of prognosis and treatment efficacy in near-real time through minimally invasive liquid biopsies. To realize this potential, however, methods for molecular analysis of these rare cells must be developed and validated. Here, we describe the integration of imaging mass cytometry (IMC) using metal-labeled antibodies as implemented on the Fluidigm Hyperion Imaging System into the workflow of the previously established High Definition Single Cell Analysis (HD-SCA) assay for liquid biopsies, along with methods for image analysis and signal normalization. Using liquid biopsies from a metastatic prostate cancer... (More)
Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation of prognosis and treatment efficacy in near-real time through minimally invasive liquid biopsies. To realize this potential, however, methods for molecular analysis of these rare cells must be developed and validated. Here, we describe the integration of imaging mass cytometry (IMC) using metal-labeled antibodies as implemented on the Fluidigm Hyperion Imaging System into the workflow of the previously established High Definition Single Cell Analysis (HD-SCA) assay for liquid biopsies, along with methods for image analysis and signal normalization. Using liquid biopsies from a metastatic prostate cancer case, we demonstrate that IMC can extend the reach of CTC characterization to include dozens of protein biomarkers, with the potential to understand a range of biological properties that could affect therapeutic response, metastasis and immune surveillance when coupled with simultaneous phenotyping of thousands of leukocytes.
(Less)
- author
- publishing date
- 2018-03
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Convergent science physical oncology
- volume
- 4
- issue
- 1
- external identifiers
-
- pmid:30906572
- ISSN
- 2057-1739
- DOI
- 10.1088/2057-1739/aaa013
- language
- English
- LU publication?
- no
- id
- f6eec3c9-12d2-41e5-95b1-f3af6e9ea11d
- date added to LUP
- 2020-03-09 12:43:22
- date last changed
- 2020-03-10 14:18:29
@article{f6eec3c9-12d2-41e5-95b1-f3af6e9ea11d, abstract = {{<p>Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation of prognosis and treatment efficacy in near-real time through minimally invasive liquid biopsies. To realize this potential, however, methods for molecular analysis of these rare cells must be developed and validated. Here, we describe the integration of imaging mass cytometry (IMC) using metal-labeled antibodies as implemented on the Fluidigm Hyperion Imaging System into the workflow of the previously established High Definition Single Cell Analysis (HD-SCA) assay for liquid biopsies, along with methods for image analysis and signal normalization. Using liquid biopsies from a metastatic prostate cancer case, we demonstrate that IMC can extend the reach of CTC characterization to include dozens of protein biomarkers, with the potential to understand a range of biological properties that could affect therapeutic response, metastasis and immune surveillance when coupled with simultaneous phenotyping of thousands of leukocytes.</p>}}, author = {{Gerdtsson, Erik and Pore, Milind and Thiele, Jana-Aletta and Gerdtsson, Anna Sandström and Malihi, Paymaneh D and Nevarez, Rafael and Kolatkar, Anand and Velasco, Carmen Ruiz and Wix, Sophia and Singh, Mohan and Carlsson, Anders and Zurita, Amado J and Logothetis, Christopher and Merchant, Akil A and Hicks, James and Kuhn, Peter}}, issn = {{2057-1739}}, language = {{eng}}, number = {{1}}, series = {{Convergent science physical oncology}}, title = {{Multiplex protein detection on circulating tumor cells from liquid biopsies using imaging mass cytometry}}, url = {{http://dx.doi.org/10.1088/2057-1739/aaa013}}, doi = {{10.1088/2057-1739/aaa013}}, volume = {{4}}, year = {{2018}}, }